Detailed view for TGME49_297110

Basic information

TDR Targets ID: 263206
Toxoplasma gondii, kinesin motor domain-containing protein
Source Database / ID:  ToxoDB 

pI: 6.8394 | Length (AA): 1660 | MW (Da): 177016 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00225   Kinesin motor domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008017   microtubule binding  
GO:0005524   ATP binding  
GO:0003777   microtubule motor activity  
GO:0007018   microtubule-based movement  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
28 615 4ozq (A) 0 734 35.00 0 1 0.252817 1.04
154 621 3wpn (A) 18 363 51.00 0 1 0.275528 0.49
154 646 2y65 (B) 12 362 36.00 0 1 0.070888 1.34
226 627 3zfd (A) 45 347 46.00 0 1 0.393769 0.21
665 851 5cwg (A) 8 192 14.00 0 0.07 0.265551 -0.89
665 921 5cwm (A) 8 226 27.00 0.26 0.98 0.295419 0.3
699 938 5cwi (A) 9 231 27.00 0.045 0.95 0.361178 0.31
702 910 5cwp (A) 6 215 28.00 0.17 0.41 0.280504 0.09
1493 1660 5cwp (A) 2 171 16.00 0.19 0.06 0.312705 -0.94

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Bradyzoite. Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128460)

Species Accession Gene Product
Arabidopsis thaliana AT2G36200   kinesin family protein
Arabidopsis thaliana AT2G28620   kinesin motor protein-related protein
Arabidopsis thaliana AT2G37420   ATP binding microtubule motor family protein
Arabidopsis thaliana AT3G45850   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Brugia malayi Bm1_27915   Kinesin motor domain containing protein
Candida albicans CaO19.712   kinesin motor domain protein similar to S. cerevisiae KIP1 (YBL063W) microtubule motor complex protein
Candida albicans CaO19.8331   kinesin motor domain protein similar to S. cerevisiae KIP1 (YBL063W) microtubule motor complex protein
Caenorhabditis elegans CELE_F23B12.8   Protein BMK-1, isoform B
Cryptosporidium hominis Chro.60483   kinesin-like boursin
Cryptosporidium parvum cgd6_4210   kinesin-like boursin, putative
Dictyostelium discoideum DDB_G0288361   kinesin family member 13
Drosophila melanogaster Dmel_CG9191   Kinesin-like protein at 61F
Echinococcus granulosus EgrG_001180200   kinesin family 1
Entamoeba histolytica EHI_124890   kinesin, putative
Echinococcus multilocularis EmuJ_001180200   kinesin family 1
Giardia lamblia GL50803_16425   Kinesin-5
Homo sapiens ENSG00000138160   kinesin family member 11
Loa Loa (eye worm) LOAG_04424   kinesin-like protein KLP2
Mus musculus ENSMUSG00000012443   kinesin family member 11
Neospora caninum NCLIV_006210   hypothetical protein
Oryza sativa 9268593   Os05g0117798
Oryza sativa 9271005   Os08g0558400
Plasmodium berghei PBANKA_0807700   kinesin-5, putative
Plasmodium falciparum PF3D7_0317500   kinesin-5
Plasmodium knowlesi PKNH_0824300   kinesin-5, putative
Plasmodium vivax PVX_095355   kinesin-5
Plasmodium yoelii PY02733   Kinesin motor domain, putative
Saccharomyces cerevisiae YBL063W   Kip1p
Schistosoma japonicum Sjp_0035130   ko:K10398 kinesin family member 11, putative
Schistosoma mansoni Smp_149260   kinesin eg-5
Schmidtea mediterranea mk4.002094.08  
Toxoplasma gondii TGME49_297110   kinesin motor domain-containing protein

Essentiality

TGME49_297110 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_297110 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens kinesin family member 11 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_297110 (Toxoplasma gondii), kinesin motor domain-containing protein
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