Detailed view for TGME49_225990

Basic information

TDR Targets ID: 263248
Toxoplasma gondii, acyl transferase domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.3144 | Length (AA): 502 | MW (Da): 53257 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00698   Acyl transferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016740   transferase activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

Fatty acid biosynthesis (KEGG)
Global map (KEGG)
Fatty acid metabolism (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 310 2cuy (A) 16 291 39.00 0 1 1.34 -0.59
1 310 1mla () 18 298 35.00 0 1 1.33 -0.74
1 495 4mz0 (A) 382 875 18.00 0 1 1.05176 1.07
177 502 3h0p (A) 2 298 36.00 0 1 1.0461 -0.54
179 502 3qat (A) 3 300 36.00 0 1 1.06992 -0.77
182 501 3hjv (A) 12 305 43.00 0 1 1.08455 -0.37

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_127857)

Species Accession Gene Product
Arabidopsis thaliana AT2G30200   [acyl-carrier-protein] S-malonyltransferase
Candida albicans CaO19.6643   similar to bacterial/mitochondrial malonyl CoA:acyl carrier transacylase
Candida albicans CaO19.13964   similar to bacterial/mitochondrial malonyl CoA:acyl carrier transacylase
Caenorhabditis elegans CELE_C50D2.9   Protein C50D2.9
Chlamydia trachomatis CT_238   malonyl CoA-ACP transacylase
Dictyostelium discoideum DDB_G0270558   hypothetical protein
Drosophila melanogaster Dmel_CG7842   bad egg
Escherichia coli b1092   malonyl-CoA-[acyl-carrier-protein] transacylase
Echinococcus granulosus EgrG_000839400   Malonyl coenzyme A acyl carrier protein transacylase
Echinococcus multilocularis EmuJ_000839400   Malonyl coenzyme A acyl carrier protein transacylase
Homo sapiens ENSG00000100294   malonyl CoA:ACP acyltransferase (mitochondrial)
Mycobacterium leprae ML1653   MALONYL CoA-ACYL CARRIER PROTEIN TRANSACYLASE FABD (Malonyl CoA:AcpM acyltransferase) (MCT)
Mus musculus ENSMUSG00000048755   malonyl CoA:ACP acyltransferase (mitochondrial)
Mycobacterium tuberculosis Rv2243   Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT)
Mycobacterium ulcerans MUL_1306   malonyl CoA-acyl carrier protein transacylase FabD
Neospora caninum NCLIV_046840   Acyl-carrier-protein S-malonyltransferase (EC 2.3.1.39), related
Oryza sativa 4332547   Os03g0297400
Plasmodium berghei PBANKA_1410500   malonyl CoA-acyl carrier protein transacylase, putative
Plasmodium falciparum PF3D7_1312000   malonyl CoA-acyl carrier protein transacylase
Plasmodium knowlesi PKNH_1412700   malonyl CoA-acyl carrier protein transacylase, putative
Plasmodium vivax PVX_122435   malonyl CoA-acyl carrier protein transacylase precursor, putative
Plasmodium yoelii PY05492   malonyl CoA-acyl carrier protein transacylase precursor
Schistosoma japonicum Sjp_0302820   ko:K00645 [acyl-carrier-protein] S-malonyltransferase [EC2.3.1.39], putative
Schistosoma mansoni Smp_072090.2   [ACP] S-malonyltransferase
Schistosoma mansoni Smp_072090.3   [ACP] S-malonyltransferase
Schistosoma mansoni Smp_072090.4   [ACP] S-malonyltransferase
Schistosoma mansoni Smp_072090.1   [ACP] S-malonyltransferase
Schmidtea mediterranea mk4.013551.00   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Schmidtea mediterranea mk4.052433.00  
Schmidtea mediterranea mk4.005521.05   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Schmidtea mediterranea mk4.004448.02   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Schmidtea mediterranea mk4.010055.00   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Toxoplasma gondii TGME49_225990   acyl transferase domain-containing protein
Wolbachia endosymbiont of Brugia malayi Wbm0402   ACP S-malonyltransferase

Essentiality

TGME49_225990 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b1092 Escherichia coli essential goodall
PBANKA_1410500 Plasmodium berghei Dispensable plasmo
TGME49_225990 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_225990 (Toxoplasma gondii), acyl transferase domain-containing protein
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