Detailed view for TGME49_205440

Basic information

TDR Targets ID: 263315
Toxoplasma gondii, CCT chaperonin gamma subunit
Source Database / ID:  ToxoDB 

pI: 5.8561 | Length (AA): 556 | MW (Da): 60613 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00118   TCP-1/cpn60 chaperonin family

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0044267   cellular protein metabolic process  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 530 4b2t (G) 7 525 61.00 0 1 1.56055 0
17 528 1a6d (A) 17 519 42.00 0 1 1.46976 -0.49
218 370 1e0r (B) 214 365 43.00 0 1 0.86908 -0.74

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127483)

Species Accession Gene Product
Arabidopsis thaliana AT5G26360   TCP-1/cpn60 chaperonin family protein
Babesia bovis BBOV_III002780   TCP-1/cpn60 chaperonin family protein
Brugia malayi Bm1_09595   T-complex protein 1, gamma subunit
Candida albicans CaO19.11487   similar to S. cerevisiae CCT3 (YJL014W) subunit of chaperonin Cct ring complex involved in cytoskeleton assembly and protein fol
Candida albicans CaO19.4004   similar to S. cerevisiae CCT3 (YJL014W) subunit of chaperonin Cct ring complex involved in cytoskeleton assembly and protein fol
Caenorhabditis elegans CELE_F54A3.3   Protein CCT-3
Cryptosporidium hominis Chro.40439   CCT chaperonin gamma subunit
Cryptosporidium parvum cgd4_3850   putative t-complex protein 1, gamma subunit
Dictyostelium discoideum DDB_G0281741   chaperonin containing TCP1 gamma subunit
Drosophila melanogaster Dmel_CG8977   CG8977 gene product from transcript CG8977-RB
Echinococcus granulosus EgrG_000872100   t complex protein 1 subunit gamma
Entamoeba histolytica EHI_158250   T-complex protein 1 gamma subunit, putative
Echinococcus multilocularis EmuJ_000872100   t complex protein 1 subunit gamma
Giardia lamblia GL50803_17411   TCP-1 chaperonin subunit gamma
Homo sapiens ENSG00000163468   chaperonin containing TCP1, subunit 3 (gamma)
Leishmania braziliensis LbrM.23.1320   T-complex protein 1, gamma subunit, putative
Leishmania donovani LdBPK_231460.1   T-complex protein 1, gamma subunit, putative
Leishmania infantum LinJ.23.1460   T-complex protein 1, gamma subunit, putative
Leishmania major LmjF.23.1220   T-complex protein 1, gamma subunit, putative
Leishmania mexicana LmxM.23.1220   T-complex protein 1, gamma subunit, putative
Loa Loa (eye worm) LOAG_11478   T-complex protein 1
Loa Loa (eye worm) LOAG_01621   hypothetical protein
Mus musculus ENSMUSG00000001416   chaperonin containing Tcp1, subunit 3 (gamma)
Neospora caninum NCLIV_020250   hypothetical protein
Oryza sativa 4341219   Os06g0538000
Oryza sativa 4328872   Os02g0247200
Plasmodium berghei PBANKA_1444100   T-complex protein 1 subunit gamma, putative
Plasmodium falciparum PF3D7_1229500   T-complex protein 1 subunit gamma
Plasmodium knowlesi PKNH_1448800   T-complex protein 1 subunit gamma, putative
Plasmodium vivax PVX_124100   T-complex protein 1 subunit gamma, putative
Plasmodium yoelii PY07337   CCT chaperonin gamma subunit
Saccharomyces cerevisiae YJL014W   Cct3p
Schistosoma japonicum Sjp_0000740   ko:K09495 T-complex protein 1 subunit gamma, putative
Schistosoma japonicum Sjp_0000730   T-complex protein 1 subunit gamma, putative
Schistosoma mansoni Smp_041740   chaperonin containing t-complex protein 1 gamma subunit tcpg
Schmidtea mediterranea mk4.023824.00   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.011090.02   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.003001.00   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.005078.00   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.023888.02   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.021158.02   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.023824.01   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.017274.01   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.005700.00   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.006034.01   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.014865.00   T-complex protein 1 subunit gamma
Schmidtea mediterranea mk4.000478.07   T-complex protein 1 subunit gamma
Trypanosoma brucei gambiense Tbg972.8.2880   T-complex protein 1, gamma subunit, putative
Trypanosoma brucei Tb927.8.3150   T-complex protein 1, gamma subunit, putative
Trypanosoma congolense TcIL3000_8_3150   T-complex protein 1, gamma subunit, putative
Trypanosoma cruzi TcCLB.504003.60   chaperonin/T-complex protein 1 gamma subunit, putative
Toxoplasma gondii TGME49_205440   CCT chaperonin gamma subunit
Theileria parva TP04_0089   T-complex protein 1, gamma subunit, putative
Trichomonas vaginalis TVAG_029280   chaperonin, putative

Essentiality

TGME49_205440 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3150 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.3150 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.3150 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.3150 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F54A3.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_F54A3.3 Caenorhabditis elegans larval arrest wormbase
CELE_F54A3.3 Caenorhabditis elegans sterile wormbase
YJL014W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1444100 Plasmodium berghei Essential plasmo
TGME49_205440 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Macaaca fascicularis T-complex protein 1 subunit beta 535 aa 29.2% 531 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_205440 (Toxoplasma gondii), CCT chaperonin gamma subunit
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