Detailed view for TGME49_204480

Basic information

TDR Targets ID: 263317
Toxoplasma gondii, thioredoxin domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.7451 | Length (AA): 659 | MW (Da): 72203 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00085   Thioredoxin
PF00226   DnaJ domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0031072   heat shock protein binding  
GO:0045454   cell redox homeostasis  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
121 187 3ucs (C) 2 70 43.00 0 1 0.856169 -2.8
123 386 3apq (A) 34 240 36.00 0 1 0.543907 0.1
123 219 4j80 (A) 5 104 46.00 0 1 0.863493 -1.93
213 255 3bog (C) 3 76 61.00 0.0013 0.26 0.34555 1.59
263 629 1a8y (A) 5 347 13.00 0 0.88 0.511404 0.58
296 658 3f8u (A) 51 385 19.00 0.0000018 0.99 0.640635 0.77
519 657 5crw (A) 208 344 16.00 0.00016 0.35 0.402726 -0.49
554 631 3bj5 (A) 252 332 18.00 0 0.52 0.321861 -0.62

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_135569)

Species Accession Gene Product
Arabidopsis thaliana AT3G08970   J domain protein ATERDJ3A
Babesia bovis BBOV_III007840   dnaJ domain containing protein
Cryptosporidium hominis Chro.20339   heat shock protein DnaJ Pfj2
Cryptosporidium parvum cgd2_3230   heat shock protein DnaJ Pfj2, putative
Neospora caninum NCLIV_020770   hypothetical protein
Oryza sativa 4332515   Os03g0293000
Plasmodium berghei PBANKA_0938300   heat shock protein, putative
Plasmodium falciparum PF3D7_1108700   heat shock protein DnaJ homologue Pfj2
Plasmodium knowlesi PKNH_0906300   heat shock protein DnaJ homologue Pfj2, putative
Plasmodium vivax PVX_091110   heat shock protein DnaJ homologue Pfj2, putative
Plasmodium yoelii PY07174   heat shock protein DnaJ homologue Pfj2
Toxoplasma gondii TGME49_204480   thioredoxin domain-containing protein
Theileria parva TP04_0688   heat shock protein DnaJ, putative

Essentiality

TGME49_204480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0938300 Plasmodium berghei Essential plasmo
TGME49_204480 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Protein Disulfide-Isomerase (5.3.4.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

4 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_204480 (Toxoplasma gondii), thioredoxin domain-containing protein
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