Detailed view for TGME49_223480

Basic information

TDR Targets ID: 263368
Toxoplasma gondii, sushi domain (scr repeat) domain-containing protein
Source Database / ID:  ToxoDB 

pI: 5.4694 | Length (AA): 4752 | MW (Da): 507031 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00066   LNR domain
PF00090   Thrombospondin type 1 domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0030154   cell differentiation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
64 96 2v70 (A) 674 714 30.00 0.00051 0.05 -0.0989556 1.42
451 485 2oo4 (A) 1438 1467 60.00 0.013 0.28 0.322065 1.45
529 583 1szl (A) 441 495 38.00 0.00076 0.96 0.500274 -0.51
529 637 1lsl (A) 416 528 38.00 0 0.99 0.291638 0.24
1942 1999 2oo4 (A) 1436 1492 37.00 0.017 0.28 0.215305 0.76
3173 3392 5cwi (A) 6 228 16.00 0.24 0.06 0.152396 -0.39
4066 4226 1rid (A) 51 244 30.00 0.52 0.3 -0.12342 1.89
4613 4750 5cwq (A) 5 148 23.00 0.44 0.04 0.28714 -1.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_131474)

Species Accession Gene Product
Cryptosporidium hominis Chro.50029   TSP1 domain-containing protein TSP2 precursor
Cryptosporidium parvum cgd5_3420   TRAP-C2 extracellular protein
Homo sapiens ENSG00000005108   thrombospondin, type I, domain containing 7A
Homo sapiens ENSG00000144229   thrombospondin, type I, domain containing 7B
Mus musculus ENSMUSG00000042581   thrombospondin, type I, domain containing 7B
Mus musculus ENSMUSG00000032625   thrombospondin, type I, domain containing 7A
Neospora caninum NCLIV_048990   thrombospondin type 1 domain-containing protein, putative
Schmidtea mediterranea mk4.023720.00  
Schmidtea mediterranea mk4.005969.04  
Schmidtea mediterranea mk4.000213.01  
Schmidtea mediterranea mk4.002273.02  
Toxoplasma gondii TGME49_223480   sushi domain (scr repeat) domain-containing protein

Essentiality

TGME49_223480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_223480 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_223480 (Toxoplasma gondii), sushi domain (scr repeat) domain-containing protein
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