Detailed view for TGME49_249950

Basic information

TDR Targets ID: 263374
Toxoplasma gondii, Mak16 protein
Source Database / ID:  ToxoDB 

pI: 5.1027 | Length (AA): 333 | MW (Da): 38805 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01778   Ribosomal L28e protein family
PF04874   Mak16 protein C-terminal region

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
64 255 2d4c (D) 21 232 13.00 0 0.01 0.601177 0.08
85 149 4wij (A) 511 577 31.00 0.066 0.16 0.610195 -1.29
212 256 5chl (A) 24 68 42.00 0.029 0.07 0.497135 0.55

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite, ME49 Bradyzoite. Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128004)

Species Accession Gene Product
Arabidopsis thaliana AT1G23280   MAK16 protein-like protein
Babesia bovis BBOV_III001370   Mak16 family protein
Brugia malayi Bm1_17915   Mak16 protein
Candida albicans CaO19.12955   Nuclear protein with HMG-like acidic region
Candida albicans CaO19.5500   Nuclear protein with HMG-like acidic region
Caenorhabditis elegans CELE_C16A3.6   Protein C16A3.6
Cryptosporidium hominis Chro.60219   hypothetical protein
Cryptosporidium parvum cgd6_1820   conserved hypothetical protein
Dictyostelium discoideum DDB_G0269642   MAK16-like protein
Drosophila melanogaster Dmel_CG10648   RNA-binding motif protein 13
Echinococcus granulosus EgrG_000830400   maintenance of killer 16 mak16 protein
Entamoeba histolytica EHI_198690   MAK16 protein, putative
Echinococcus multilocularis EmuJ_000830400   maintenance of killer 16 (mak16) protein
Giardia lamblia GL50803_5661   RNA binding protein
Homo sapiens ENSG00000198042   MAK16 homolog (S. cerevisiae)
Leishmania braziliensis LbrM.22.1260   hypothetical protein, conserved,mak-16-like RNA binding protein, putative
Leishmania donovani LdBPK_221230.1   hypothetical protein, conserved
Leishmania infantum LinJ.22.1230   hypothetical protein, conserved,mak-16-like RNA binding protein, putative
Leishmania major LmjF.22.1380   hypothetical protein, conserved,mak-16-like RNA binding protein, putative
Leishmania mexicana LmxM.22.1380   hypothetical protein, conserved,mak-16-like RNA binding protein, putative
Loa Loa (eye worm) LOAG_05336   MAK16 protein
Mus musculus ENSMUSG00000031578   MAK16 homolog (S. cerevisiae)
Neospora caninum NCLIV_066060   MAK16 protein, putative
Oryza sativa 4342894   Os07g0270900
Onchocerca volvulus OVOC8890   Protein MAK16 homolog
Plasmodium berghei PBANKA_0301500   protein MAK16, putative
Plasmodium falciparum PF3D7_0203700   protein MAK16, putative
Plasmodium knowlesi PKNH_0417400   protein MAK16, putative
Plasmodium vivax PVX_003615   hypothetical protein, conserved
Plasmodium yoelii PY01531   hypothetical protein
Saccharomyces cerevisiae YAL025C   Mak16p
Schistosoma japonicum Sjp_0203340   MAK16 protein homolog, putative
Schistosoma mansoni Smp_050360.1   MAK16 protein homolog (SmMAK16)
Schmidtea mediterranea mk4.013054.01   Protein MAK16 homolog
Trypanosoma brucei gambiense Tbg972.7.3710   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.3380   Ribosomal L28e protein family/Mak16 protein C-terminal region, putative
Trypanosoma congolense TcIL3000_7_2590   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508323.30   mak-16-like RNA binding protein, putative
Trypanosoma cruzi TcCLB.511825.10   mak-16-like RNA binding protein, putative
Toxoplasma gondii TGME49_249950   Mak16 protein
Theileria parva TP03_0704   hypothetical protein, conserved
Trichomonas vaginalis TVAG_355000   maintenance of killer 16 (mak16) protein, putative

Essentiality

TGME49_249950 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3380 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.3380 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.3380 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.3380 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C16A3.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_C16A3.6 Caenorhabditis elegans larval arrest wormbase
CELE_C16A3.6 Caenorhabditis elegans slow growth wormbase
YAL025C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0301500 Plasmodium berghei Essential plasmo
TGME49_249950 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for GST 1-Chloro-2,4-Dinitrobenzene as Substrate (2.5.1.18 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

11 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_249950 (Toxoplasma gondii), Mak16 protein
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