Detailed view for TGME49_289800

Basic information

TDR Targets ID: 263457
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 10.2315 | Length (AA): 234 | MW (Da): 25883 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01105   emp24/gp25L/p24 family/GOLD

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
48 147 5azx (A) 31 128 17.00 0.000025 0.11 0.55165 0.28
112 165 2qp2 (A) 314 366 32.00 0.53 0.02 0.389969 1.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127900)

Species Accession Gene Product
Arabidopsis thaliana AT3G10780   emp24/gp25L/p24 family/GOLD domain-containing protein
Arabidopsis thaliana AT1G57620   emp24/gp25L/p24 family/GOLD domain-containing protein
Arabidopsis thaliana AT1G21900   p24 subfamily delta 5
Arabidopsis thaliana AT1G09580   transmembrane emp24 domain-containing protein p24delta3
Babesia bovis BBOV_III006340   membrane protein, putative
Brugia malayi Bm1_55295   Transmembrane protein
Candida albicans CaO19.7409   Component of COPII coat of ER-derived vesicles
Caenorhabditis elegans CELE_F47G9.1   Protein F47G9.1
Cryptosporidium parvum cgd1_430   possible emp24/gp25L/p24 family protein, transmembrane domain
Dictyostelium discoideum DDB_G0277923   hypothetical protein
Drosophila melanogaster Dmel_CG11785   baiser
Echinococcus granulosus EgrG_000544600   transmembrane emp24 domain containing protein
Entamoeba histolytica EHI_058320   transmembrane protein tmp21, putative
Echinococcus multilocularis EmuJ_000544600   transmembrane emp24 domain containing protein
Homo sapiens ENSG00000170348   transmembrane emp24-like trafficking protein 10 (yeast)
Leishmania braziliensis LbrM.34.1760   COP-coated vesicle membrane protein gp25L precursor, putative,ER--golgi transport protein gp25L, putative
Leishmania donovani LdBPK_351840.1   COP-coated vesicle membrane protein gp25L precursor, putative
Leishmania infantum LinJ.35.1840   COP-coated vesicle membrane protein gp25L precursor, putative,ER--golgi transport protein gp25L, putative
Leishmania major LmjF.35.1850   COP-coated vesicle membrane protein gp25L precursor, putative,ER--golgi transport protein gp25L, putative
Leishmania mexicana LmxM.34.1850   COP-coated vesicle membrane protein gp25L precursor, putative,ER--golgi transport protein gp25L, putative
Loa Loa (eye worm) LOAG_00194   transmembrane protein
Mus musculus ENSMUSG00000021248   transmembrane emp24-like trafficking protein 10 (yeast)
Neospora caninum NCLIV_042050   hypothetical protein
Oryza sativa 4330850   Os02g0768200
Oryza sativa 4332955   Os03g0376000
Oryza sativa 4340456   Os06g0210100
Oryza sativa 4344005   Os07g0632700
Plasmodium berghei PBANKA_1348000   transmembrane protein Tmp21 homologue, putative
Plasmodium falciparum PF3D7_1333300   transmembrane protein Tmp21 homologue, putative
Plasmodium knowlesi PKNH_1267300   transmembrane protein Tmp21 homologue, putative
Plasmodium vivax PVX_082595   hypothetical protein, conserved
Plasmodium yoelii PY06414   transmembrane protein tmp21 precursor
Saccharomyces cerevisiae YML012W   Erv25p
Schistosoma japonicum Sjp_0025150   Transmembrane emp24 domain-containing protein 10 precursor, putative
Schistosoma mansoni Smp_079310   transmembrane protein tmp21-related
Schmidtea mediterranea mk4.010203.00   Transmembrane protein tmp21-related
Trypanosoma brucei gambiense Tbg972.9.9350   COP-coated vesicle membrane protein gp25L precursor, putative,ER--golgi transport protein gp25L, putative
Trypanosoma brucei Tb927.9.15090   cytosolic coat protein, putative
Trypanosoma congolense TcIL3000_9_6310   ER--golgi transport protein gp25L, putative
Trypanosoma cruzi TcCLB.510765.30   COP-coated vesicle membrane protein gp25L precursor, putative
Trypanosoma cruzi TcCLB.509671.90   ER--golgi transport protein gp25L, putative
Toxoplasma gondii TGME49_289800   hypothetical protein
Theileria parva TP02_0330   hypothetical protein

Essentiality

TGME49_289800 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.244.2760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.244.2760 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.244.2760 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.244.2760 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F47G9.1 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1348000 Plasmodium berghei Dispensable plasmo
TGME49_289800 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_289800 (Toxoplasma gondii), hypothetical protein
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