Detailed view for TGME49_234490

Basic information

TDR Targets ID: 263476
Toxoplasma gondii, kelch repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 7.7042 | Length (AA): 1503 | MW (Da): 164472 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02475   Met-10+ like-protein
PF02676   Methyltransferase TYW3

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
320 617 2frn (A) 20 275 23.00 0 1 0.23 0.45
404 549 1dus (A) 16 152 17.00 0.0000000012 0.78 0.43 -0.56
74 332 2it2 (A) 21 197 34.00 0 1 -0.031978 0.41
136 217 3fkc (A) 11 86 5.00 0.00096 0 0.0880576 -0.7
137 217 3ga1 (A) 10 86 12.00 0.00074 0.03 0.191392 -0.81
449 746 2dyh (A) 328 615 15.00 0 0.85 0.31197 -0.12
1102 1375 3k6r (A) 15 234 34.00 0 1 0.192002 1.01
1212 1312 3dr5 (A) 34 132 12.00 0 0.43 0.216899 -0.3
1234 1310 1g55 (A) 2 76 17.00 0 0.13 0.165931 0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_129346)

Species Accession Gene Product
Arabidopsis thaliana AT4G04670   Met-10+ like family protein / kelch repeat-containing protein
Brugia malayi Bm1_51625   Met-10+ like-protein
Candida albicans CaO19.7369   similar to S. cerevisiae YML005W
Caenorhabditis elegans CELE_F40F9.10   Protein F40F9.10
Homo sapiens ENSG00000183665   tRNA methyltransferase 12 homolog (S. cerevisiae)
Leishmania braziliensis LbrM.03.0070   hypothetical protein, conserved
Leishmania donovani LdBPK_030050.1   Met-10+ like-protein, putative
Leishmania infantum LinJ.03.0050   hypothetical protein, conserved
Leishmania major LmjF.03.0060   hypothetical protein, conserved
Leishmania mexicana LmxM.03.0060   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02567   hypothetical protein
Loa Loa (eye worm) LOAG_14175   hypothetical protein
Mus musculus ENSMUSG00000037085   tRNA methyltranferase 12
Oryza sativa 4343372   Os07g0515000
Plasmodium berghei PBANKA_0502800   tRNA wybutosine-synthesizing protein, putative
Plasmodium falciparum PF3D7_1018600   tRNA wybutosine-synthesizing protein, putative
Plasmodium knowlesi PKNH_0602900   tRNA wybutosine-synthesizing protein, putative
Plasmodium yoelii PY05008   Unknown protein
Saccharomyces cerevisiae YML005W   Trm12p
Trypanosoma brucei gambiense Tbg972.10.3900   hypothetical protein, conserved,predicted to be involved in methionine biosynthesis
Trypanosoma brucei Tb927.10.3080   methionine biosynthetic protein, putative
Trypanosoma congolense TcIL3000_10_2530   predicted to be involved in methionine biosynthesis
Trypanosoma cruzi TcCLB.510943.180   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504867.110   hypothetical protein, conserved
Toxoplasma gondii TGME49_234490   kelch repeat-containing protein
Theileria parva TP02_0179   hypothetical protein

Essentiality

TGME49_234490 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.3080 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.3080 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.3080 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.3080 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0502800 Plasmodium berghei Essential plasmo
TGME49_234490 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_234490 (Toxoplasma gondii), kelch repeat-containing protein
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