Detailed view for TGME49_203110

Basic information

TDR Targets ID: 263489
Toxoplasma gondii, citrate synthase, putative
Source Database / ID:  ToxoDB 

pI: 8.9017 | Length (AA): 509 | MW (Da): 56604 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00285   Citrate synthase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0046912   transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer  
GO:0044262   cellular carbohydrate metabolic process  
GO:0006099   tricarboxylic acid cycle  

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 297 1a59 () 94 378 32.00 0 1 1.27 -1.55
1 310 1csc () 138 430 26.00 0 1 1.22 -0.93
1 295 1k3p (A) 131 420 42.00 0 1 1.48 -1.14
37 469 4xgh (A) 7 433 40.00 0 1 1.16519 0.21
41 469 2h12 (A) 12 436 41.00 0 1 1.36343 -0.6
50 469 4e6y (A) 15 428 40.00 0 1 1.31465 -0.43
79 464 3hwk (A) 28 390 34.00 0 1 1.17985 -0.69

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite, ME49 Oocyst. Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128590)

Species Accession Gene Product
Arabidopsis thaliana AT3G58740   citrate synthase 1
Arabidopsis thaliana AT2G42790   citrate synthase 3
Arabidopsis thaliana AT3G58750   citrate synthase 2
Dictyostelium discoideum DDB_G0276965   citrate synthase
Dictyostelium discoideum DDB_G0287281   citrate synthase
Dictyostelium discoideum DDB_G0267426   citrate synthase
Drosophila melanogaster Dmel_CG14740   CG14740 gene product from transcript CG14740-RA
Escherichia coli b0720   citrate synthase
Escherichia coli b0333   2-methylcitrate synthase
Mycobacterium leprae ML2130c   PROBABLE CITRATE SYNTHASE I GLTA2
Mycobacterium tuberculosis Rv1131   Probable methylcitrate synthase PrpC
Mycobacterium tuberculosis Rv0896   Probable citrate synthase I GltA2
Mycobacterium ulcerans MUL_0255   type II citrate synthase
Mycobacterium ulcerans MUL_2494   citrate synthase
Neospora caninum NCLIV_024780   citrate synthase, putative
Neospora caninum NCLIV_021820   Citrate synthase (EC 2.3.3.1), related
Oryza sativa 4328812   Os02g0232400
Plasmodium vivax PVX_211290   unspecified product
Plasmodium vivax PVX_226290   unspecified product
Schmidtea mediterranea mk4.054382.01  
Schmidtea mediterranea mk4.046581.00  
Toxoplasma gondii TGME49_203110   citrate synthase, putative
Toxoplasma gondii TGME49_263130   citrate synthase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0735   citrate synthase

Essentiality

TGME49_203110 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1151 Mycobacterium tuberculosis non-essential nmpdr
b0333 Escherichia coli non-essential goodall
b0720 Escherichia coli non-essential goodall
TGME49_203110 this record Toxoplasma gondii Probably non-essential sidik
TGME49_263130 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_203110 (Toxoplasma gondii), citrate synthase, putative
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