Detailed view for LmjF.31.2740

Basic information

TDR Targets ID: 26349
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.3336 | Length (AA): 467 | MW (Da): 51436 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07970   Endoplasmic reticulum vesicle transporter
PF13850   Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
116 419 3zlc (B) 51 294 25.00 0 0.81 0.364064 1.55
180 303 4aw8 (A) 17 164 31.00 0.67 0.29 0.249525 1.39

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127803)

Species Accession Gene Product
Arabidopsis thaliana AT1G36050   Endoplasmic reticulum vesicle transporter protein
Arabidopsis thaliana AT1G22200   Endoplasmic reticulum vesicle transporter protein
Brugia malayi Bm1_33800   Serologically defined breast cancer antigen NY-BR-84 homolog
Candida albicans CaO19.8218   ER-Golgi transport vesicle protein
Candida albicans CaO19.586   ER-Golgi transport vesicle protein
Caenorhabditis elegans CELE_K09E9.2   Protein ERV-46
Cryptosporidium hominis Chro.70352   serologically defined breast cancer antigen 84
Cryptosporidium parvum cgd7_3130   ER vesicle protein; Erv41p, transmembrane region near C terminus and possible N region transmembrane
Dictyostelium discoideum DDB_G0292002   endoplasmic reticulum-golgi intermediate compartment protein 3
Drosophila melanogaster Dmel_CG7011   CG7011 gene product from transcript CG7011-RA
Echinococcus granulosus EgrG_001055100   endoplasmic reticulum Golgi intermediate
Entamoeba histolytica EHI_079180   hypothetical protein, conserved
Entamoeba histolytica EHI_182970   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_001055100   endoplasmic reticulum Golgi intermediate
Homo sapiens ENSG00000125991   ERGIC and golgi 3
Leishmania braziliensis LbrM.31.3090   hypothetical protein, conserved
Leishmania donovani LdBPK_312830.1   Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)/Endoplasmic reticulum vesicle transporter, putative
Leishmania infantum LinJ.31.2830   hypothetical protein, conserved
Leishmania major LmjF.31.2740   hypothetical protein, conserved
Leishmania mexicana LmxM.30.2740   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05019   hypothetical protein
Mus musculus ENSMUSG00000005881   ERGIC and golgi 3
Oryza sativa 4336024   Os04g0455900
Oryza sativa 4339163   Os05g0490200
Saccharomyces cerevisiae YAL042W   Erv46p
Schistosoma japonicum Sjp_0019110   Endoplasmic reticulum-Golgi intermediate compartment protein 3, putative
Schistosoma mansoni Smp_040360   serologically defined breast cancer antigen ny-br-84-related
Schmidtea mediterranea mk4.012488.00   Endoplasmic reticulum-Golgi intermediate compartment protein 3
Schmidtea mediterranea mk4.007664.00  
Trypanosoma brucei gambiense Tbg972.4.5290   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.8.7570   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.7270   Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)/Endoplasmic reticulum vesicle transporter, putative
Trypanosoma brucei Tb927.4.5100   Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)/Endoplasmic reticulum vesicle transporter, putative
Trypanosoma congolense TcIL3000_0_00550   Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)/Endoplasmic reticulum vesicle transporter, putative
Trypanosoma congolense TcIL3000_8_7410   Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)/Endoplasmic reticulum vesicle transporter, putative
Trypanosoma cruzi TcCLB.510593.30   Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)/Endoplasmic reticulum vesicle transporter, putative
Trichomonas vaginalis TVAG_172950   Endoplasmic reticulum-golgi intermediate compartment protein, putative
Trichomonas vaginalis TVAG_396530   Endoplasmic reticulum-golgi intermediate compartment protein, putative
Trichomonas vaginalis TVAG_442240   er-golgi intermediate compartment 32 kD protein, putative
Trichomonas vaginalis TVAG_317730   Endoplasmic reticulum-golgi intermediate compartment protein, putative
Trichomonas vaginalis TVAG_100310   ptx1 protein, putative
Trichomonas vaginalis TVAG_177510   er-golgi intermediate compartment 32 kD protein, putative
Trichomonas vaginalis TVAG_470170   Endoplasmic reticulum-golgi intermediate compartment protein, putative
Trichomonas vaginalis TVAG_394520   Endoplasmic reticulum-golgi intermediate compartment protein, putative
Trichomonas vaginalis TVAG_036780   Endoplasmic reticulum-golgi intermediate compartment protein, putative
Trichomonas vaginalis TVAG_342940   Endoplasmic reticulum-golgi intermediate compartment protein, putative
Trichomonas vaginalis TVAG_469210   ptx1 protein, putative

Essentiality

LmjF.31.2740 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.7270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7270 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.8.7270 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.4.5100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.5100 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.5100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.5100 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.31.2740 (Leishmania major), hypothetical protein, conserved
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