Detailed view for TGME49_227070

Basic information

TDR Targets ID: 263537
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 4.7387 | Length (AA): 300 | MW (Da): 33770 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 119 5j1g (A) 1112 1228 11.00 0 0.02 0.583433 -1.62
19 156 4ilo (A) 30 152 31.00 0.2 0.38 0.6603 -0.33
19 169 4qn1 (A) 1141 1346 15.00 0 0.3 0.635433 -1.01
50 269 5cwm (A) 4 211 30.00 0.12 0.84 0.980633 -0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 merozoite, ME49 Bradyzoite. Hehl AB Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_131306)

Species Accession Gene Product
Arabidopsis thaliana AT3G02950   Tho complex subunit 7
Arabidopsis thaliana AT5G16790   protein THO7
Brugia malayi Bm1_19120   IL5 receptor binding protein
Caenorhabditis elegans CELE_B0513.2   Protein B0513.2, isoform B
Dictyostelium discoideum DDB_G0275979   hypothetical protein
Drosophila melanogaster Dmel_CG17143   CG17143 gene product from transcript CG17143-RB
Echinococcus granulosus EgrG_000252900   tho complex subunit 7
Echinococcus multilocularis EmuJ_000252900   tho complex subunit 7
Homo sapiens ENSG00000163634   THO complex 7 homolog (Drosophila)
Loa Loa (eye worm) LOAG_10082   hypothetical protein
Mus musculus ENSMUSG00000053453   THO complex 7 homolog (Drosophila)
Oryza sativa 4339657   Os05g0569800
Oryza sativa 4344717   Os08g0160000
Onchocerca volvulus OVOC3589   THO complex subunit 7 homolog
Schistosoma japonicum Sjp_0083200   THO complex subunit 7 homolog, putative
Schistosoma mansoni Smp_059810   hypothetical protein
Toxoplasma gondii TGME49_227070   hypothetical protein

Essentiality

TGME49_227070 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_B0513.2 Caenorhabditis elegans embryonic lethal wormbase
TGME49_227070 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_227070 (Toxoplasma gondii), hypothetical protein
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