Detailed view for TGME49_264760

Basic information

TDR Targets ID: 263538
Toxoplasma gondii, Oxysterol-binding protein
Source Database / ID:  ToxoDB 

pI: 8.5381 | Length (AA): 1302 | MW (Da): 138226 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00169   PH domain
PF01237   Oxysterol-binding protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0008202   steroid metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
126 219 1zm0 (A) 243 347 24.00 0 1 0.4 -1.34
128 219 1u29 (A) 260 376 23.00 0 0.89 0.17 -1.53
716 1092 1zhx (A) 0 373 22.00 0 1 0.38 0.12
288 385 1unq (A) 4 112 22.00 0 0.98 0.253769 -0.23
293 381 3pp2 (A) 500 612 17.00 0 0.55 0.109856 -1.09
294 378 4hhv (A) 28 114 28.00 0.012 0.92 0.467484 -0.88
718 754 4dmu (A) 10 208 54.00 0.28 0.04 0.494618 -0.2
872 1302 4ic4 (A) 628 996 31.00 0 1 0.442529 0.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_126646)

Species Accession Gene Product
Arabidopsis thaliana AT2G31030   oxysterol binding protein-related protein 1B
Arabidopsis thaliana AT4G22540   oxysterol binding protein-related protein 2A
Arabidopsis thaliana AT2G31020   oxysterol binding protein-related protein 1A
Arabidopsis thaliana AT4G12460   OSBP(oxysterol binding protein)-related protein 2B
Arabidopsis thaliana AT4G08180   OSBP(oxysterol binding protein)-related protein 1C
Arabidopsis thaliana AT1G13170   oxysterol binding protein-related protein 1D
Babesia bovis BBOV_III005450   oxysterol-binding family protein
Brugia malayi Bm1_34105   Oxysterol-binding protein
Brugia malayi Bm1_17010   Oxysterol-binding protein
Candida albicans CaO19.8897   likely oxysterol-binding protein similar to S. cerevisiae OSH3 (YHR073W) involved in steroid biosynthesis
Candida albicans CaO19.12561   likely oxysterol-binding protein similar to S. cerevisiae OSH2 (YDL019C) involved in ergosterol biosynthesis
Candida albicans CaO19.1317   likely oxysterol-binding protein similar to S. cerevisiae OSH3 (YHR073W) involved in steroid biosynthesis
Candida albicans CaO19.5095   likely oxysterol-binding protein similar to S. cerevisiae OSH2 (YDL019C) involved in ergosterol biosynthesis
Caenorhabditis elegans CELE_F14H8.1   Protein OBR-2, isoform A
Caenorhabditis elegans CELE_Y47D3A.17   Protein OBR-1, isoform D
Cryptosporidium hominis Chro.40290   RIKEN cDNA C130070J12 gene
Cryptosporidium hominis Chro.10042   similar to oxysterol-binding protein-like protein 3 isoform a; oxysterol-binding protein-related protein 3; oxysterol-binding pr
Cryptosporidium parvum cgd4_2580   oxysterol binding protein 1A-like pleckstrin homology (PH) domain containing protein
Cryptosporidium parvum cgd1_320   oxysterol binding protein
Dictyostelium discoideum DDB_G0288817   oxysterol binding family protein, member 11
Dictyostelium discoideum DDB_G0283709   oxysterol binding family protein, member 8
Drosophila melanogaster Dmel_CG6708   Oxysterol binding protein
Drosophila melanogaster Dmel_CG3860   CG3860 gene product from transcript CG3860-RA
Echinococcus granulosus EgrG_000366100   oxysterol binding protein 3
Echinococcus granulosus EgrG_000869500   oxysterol binding protein 1
Echinococcus granulosus EgrG_000850900   oxysterol binding protein 1
Echinococcus multilocularis EmuJ_000366100   oxysterol binding protein 3
Echinococcus multilocularis EmuJ_000869500   oxysterol binding protein 1
Echinococcus multilocularis EmuJ_000850900   oxysterol binding protein 1
Homo sapiens ENSG00000079156   oxysterol binding protein-like 6
Homo sapiens ENSG00000110048   oxysterol binding protein
Homo sapiens ENSG00000141447   oxysterol binding protein-like 1A
Homo sapiens ENSG00000184792   oxysterol binding protein 2
Homo sapiens ENSG00000070882   oxysterol binding protein-like 3
Homo sapiens ENSG00000130703   oxysterol binding protein-like 2
Homo sapiens ENSG00000006025   oxysterol binding protein-like 7
Loa Loa (eye worm) LOAG_09503   hypothetical protein
Loa Loa (eye worm) LOAG_09166   hypothetical protein
Loa Loa (eye worm) LOAG_13256   oxysterol-binding protein
Loa Loa (eye worm) LOAG_03323   hypothetical protein
Loa Loa (eye worm) LOAG_09167   hypothetical protein
Mus musculus ENSMUSG00000020435   oxysterol binding protein 2
Mus musculus ENSMUSG00000039050   oxysterol binding protein-like 2
Mus musculus ENSMUSG00000029822   oxysterol binding protein-like 3
Mus musculus ENSMUSG00000044252   oxysterol binding protein-like 1A
Mus musculus ENSMUSG00000038534   oxysterol binding protein-like 7
Mus musculus ENSMUSG00000042359   oxysterol binding protein-like 6
Mus musculus ENSMUSG00000024687   oxysterol binding protein
Neospora caninum NCLIV_040420   Oxysterol-binding family protein, related
Oryza sativa 4351995   Os12g0285600
Oryza sativa 4344687   Os08g0154900
Oryza sativa 4349338   Os10g0554100
Oryza sativa 9270471   Os03g0704700
Plasmodium berghei PBANKA_0916600   oxysterol-binding protein, putative
Plasmodium falciparum PF3D7_1131800   oxysterol-binding protein, putative
Plasmodium knowlesi PKNH_0930000   oxysterol-binding protein, putative
Plasmodium vivax PVX_092195   oxysterol-binding protein/PH domain containing protein
Plasmodium yoelii PY04188   oxysterol-binding protein-related protein 2
Saccharomyces cerevisiae YHR073W   oxysterol-binding protein related protein OSH3
Saccharomyces cerevisiae YDL019C   oxysterol-binding protein related protein OSH2
Saccharomyces cerevisiae YAR042W   oxysterol-binding protein related protein SWH1
Schistosoma japonicum Sjp_0040080   Oxysterol-binding protein 1, putative
Schistosoma japonicum Sjp_0300920   Oxysterol-binding protein 1, putative
Schistosoma japonicum Sjp_0013110   Oxysterol-binding protein-related protein 1, putative
Schistosoma japonicum Sjp_0040090   Oxysterol-binding protein 2, putative
Schistosoma japonicum Sjp_0129700   Oxysterol-binding protein 2, putative
Schistosoma japonicum Sjp_0009860   IPR000648,Oxysterol-binding protein,domain-containing
Schistosoma japonicum Sjp_0013120   expressed protein
Schistosoma japonicum Sjp_0202210   IPR000648,Oxysterol-binding protein,domain-containing
Schistosoma mansoni Smp_058980.1   oxysterol-binding protein 1
Schistosoma mansoni Smp_144850   oxysterol-binding protein 1a
Schistosoma mansoni Smp_128150   oxysterol-binding protein 1
Schistosoma mansoni Smp_058980.2   oxysterol-binding protein 1
Schmidtea mediterranea mk4.005840.00  
Schmidtea mediterranea mk4.025281.00  
Schmidtea mediterranea mk4.029407.00   Oxysterol-binding protein
Schmidtea mediterranea mk4.002482.02   Putative oxysterol-binding protein 1
Schmidtea mediterranea mk4.004011.00  
Schmidtea mediterranea mk4.020915.00   Oxysterol-binding protein
Schmidtea mediterranea mk4.003889.00   Oxysterol-binding protein
Schmidtea mediterranea mk4.001591.00   Putative oxysterol-binding protein 1
Schmidtea mediterranea mk4.028542.00  
Schmidtea mediterranea mk4.003103.02   Oxysterol-binding protein
Schmidtea mediterranea mk4.012311.00  
Schmidtea mediterranea mk4.014439.00  
Toxoplasma gondii TGME49_264760   Oxysterol-binding protein
Theileria parva TP02_0401   oxysterol-binding protein, putative

Essentiality

TGME49_264760 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_F14H8.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y47D3A.17 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0916600 Plasmodium berghei Slow plasmo
TGME49_264760 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_264760 (Toxoplasma gondii), Oxysterol-binding protein
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