Detailed view for TGME49_216260

Basic information

TDR Targets ID: 263631
Toxoplasma gondii, eukaryotic initiation factor-2B, gamma subunit, putative
Source Database / ID:  ToxoDB 

pI: 6.7034 | Length (AA): 525 | MW (Da): 58016 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12804   MobA-like NTP transferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016779   nucleotidyltransferase activity  
GO:0016740   transferase activity  
GO:0009058   biosynthetic process  

Metabolic Pathways

RNA transport (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
28 350 1lvw (A) 1 289 16.00 0 1 0.75 -0.15
56 147 1vh1 (A) 19 116 17.00 0.000041 0.48 0.43 -0.59
26 518 5b04 (E) 37 447 33.00 0 1 0.932948 0.97
422 515 3twd (A) 292 386 16.00 0.22 1 0.530548 -1.24
481 525 1ssq (A) 189 234 20.00 0.2 0.31 0.283214 -0.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128844)

Species Accession Gene Product
Arabidopsis thaliana AT5G19485   transferases/nucleotidyltransferases
Babesia bovis BBOV_II000340   hypothetical protein
Brugia malayi Bm1_42560   Nucleotidyl transferase family protein
Candida albicans CaO19.481   similar to S. cerevisiae GCD1 (YOR260W) gamma subunit of eIF-2B, the guanine nucleotide exchange factor for translation initiati
Candida albicans CaO19.8111   similar to S. cerevisiae GCD1 (YOR260W) gamma subunit of eIF-2B, the guanine nucleotide exchange factor for translation initiati
Caenorhabditis elegans CELE_C15F1.4   Protein PPP-1
Caenorhabditis elegans CELE_C15F1.3   Protein TRA-2, isoform C
Cryptosporidium hominis Chro.80452   hypothetical protein
Cryptosporidium parvum cgd8_3940   eIF-2B gamma, eukaryotic translation initiation factor 2B subunit 3 that has a nucleotide diphospho sugar transferase at the N-t
Dictyostelium discoideum DDB_G0290693   bacterial transferase hexapeptide repeat-containing protein
Drosophila melanogaster Dmel_CG8190   CG8190 gene product from transcript CG8190-RA
Echinococcus granulosus EgrG_000671300   DNA polymerase epsilon catalytic subunit
Echinococcus multilocularis EmuJ_000671300   DNA polymerase epsilon catalytic subunit
Homo sapiens ENSG00000070785   eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
Loa Loa (eye worm) LOAG_03140   hypothetical protein
Mus musculus ENSMUSG00000028683   eukaryotic translation initiation factor 2B, subunit 3
Neospora caninum NCLIV_059920   eukaryotic initiation factor-2B gamma subunit, putative
Oryza sativa 4340947   Os06g0338900
Onchocerca volvulus OVOC4928  
Plasmodium berghei PBANKA_1341600   translation initiation factor eIF-2B subunit gamma, putative
Plasmodium falciparum PF3D7_1326400   translation initiation factor eIF-2B subunit gamma, putative
Plasmodium knowlesi PKNH_1202200   translation initiation factor eIF-2B subunit gamma, putative
Plasmodium vivax PVX_116557   translation initiation factor EIF-2B gamma subunit, putative
Plasmodium yoelii PY05721   hypothetical protein
Plasmodium yoelii PY05722   hypothetical protein
Saccharomyces cerevisiae YOR260W   Gcd1p
Schistosoma japonicum Sjp_0213650   ko:K03241 translation initiation factor eIF-2B gamma subunit, putative
Toxoplasma gondii TGME49_216260   eukaryotic initiation factor-2B, gamma subunit, putative
Theileria parva TP01_0593   hypothetical protein, conserved

Essentiality

TGME49_216260 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_C15F1.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C15F1.4 Caenorhabditis elegans larval arrest wormbase
CELE_C15F1.4 Caenorhabditis elegans larval lethal wormbase
CELE_C15F1.4 Caenorhabditis elegans slow growth wormbase
CELE_C15F1.4 Caenorhabditis elegans sterile wormbase
YOR260W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1341600 Plasmodium berghei Essential plasmo
TGME49_216260 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_216260 (Toxoplasma gondii), eukaryotic initiation factor-2B, gamma subunit, putative
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