Detailed view for TGME49_223710

Basic information

TDR Targets ID: 263758
Toxoplasma gondii, tetratricopeptide repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 8.7699 | Length (AA): 964 | MW (Da): 109194 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07719   Tetratricopeptide repeat
PF12569   NMDA receptor-regulated protein 1

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 783 4kvm (A) 6 682 33.00 0 1 0.955555 0.56
15 143 2xcb (A) 33 159 10.00 0 0.85 0.395817 -1.17
22 854 4xnh (A) 24 784 24.00 0 1 0.812108 0.88
50 139 3ma5 (A) 59 148 17.00 0 0.92 0.428361 -1.42
793 955 5cwf (A) 4 173 9.00 0.77 0.13 0.265387 -0.73

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127529)

Species Accession Gene Product
Arabidopsis thaliana AT1G80410   protein EMBRYO DEFECTIVE 2753
Babesia bovis BBOV_IV011250   tetratricopeptide repeat containing domain protein
Brugia malayi Bm1_44550   TPR Domain containing protein
Candida albicans CaO19.10695   protein N-acetyltransferase subunit
Candida albicans CaO19.3185   protein N-acetyltransferase subunit
Caenorhabditis elegans CELE_Y50D7A.4   Protein HPO-29
Cryptosporidium hominis Chro.70115   hypothetical protein
Cryptosporidium hominis Chro.70114   hypothetical protein
Cryptosporidium parvum cgd7_920   protein with 2 possible TPR domains, possible n-terminal acetyltransferase
Dictyostelium discoideum DDB_G0275075   tetratricopeptide-like helical domain-containing protein
Drosophila melanogaster Dmel_CG12202   NAT1 ortholog (S. cerevisiae)
Echinococcus granulosus EgrG_000817600   n alpha acetyltransferase 15 NatA auxiliary
Entamoeba histolytica EHI_000930   hypothetical protein, conserved protein
Entamoeba histolytica EHI_098390   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000817600   n alpha acetyltransferase 15, NatA auxiliary
Homo sapiens ENSG00000164134   N(alpha)-acetyltransferase 15, NatA auxiliary subunit
Homo sapiens ENSG00000172766   N(alpha)-acetyltransferase 16, NatA auxiliary subunit
Leishmania braziliensis LbrM.35.1450   N-acetyltransferase subunit Nat1, putative
Leishmania donovani LdBPK_361390.1   N-acetyltransferase subunit Nat1, putative
Leishmania infantum LinJ.36.1390   N-acetyltransferase subunit Nat1, putative
Leishmania major LmjF.36.1340   N-acetyltransferase subunit Nat1, putative
Leishmania mexicana LmxM.36.1340   N-acetyltransferase subunit Nat1, putative
Loa Loa (eye worm) LOAG_07419   hypothetical protein
Loa Loa (eye worm) LOAG_06579   hypothetical protein
Mus musculus ENSMUSG00000022020   N(alpha)-acetyltransferase 16, NatA auxiliary subunit
Mus musculus ENSMUSG00000063273   N(alpha)-acetyltransferase 15, NatA auxiliary subunit
Neospora caninum NCLIV_048770   N-terminal acetyltransferase complex subunit NARG1, putative
Oryza sativa 4326606   Os01g0617500
Plasmodium berghei PBANKA_1457400   N-alpha-acetyltransferase 15, NatA auxiliary subunit, putative
Plasmodium falciparum PF3D7_1244100   N-alpha-acetyltransferase 15, NatA auxiliary subunit, putative
Plasmodium knowlesi PKNH_1463400   N-alpha-acetyltransferase 15, NatA auxiliary subunit, putative
Plasmodium vivax PVX_101105   hypothetical protein, conserved
Plasmodium yoelii PY01215   unnamed protein product
Plasmodium yoelii PY06007   acetyltransferase Tubedown-1
Saccharomyces cerevisiae YDL040C   Nat1p
Schistosoma japonicum Sjp_0123290   ko:K00670 peptide alpha-N-acetyltransferase [EC2.3.1.88], putative
Schistosoma japonicum Sjp_0310150   NMDA receptor-regulated protein 1, putative
Schistosoma japonicum Sjp_0305930   NMDA receptor-regulated 1-like protein, putative
Schistosoma mansoni Smp_062520   n-terminal acetlytransferase-related
Schmidtea mediterranea mk4.036643.00   N-terminal acetlytransferase-related
Schmidtea mediterranea mk4.006548.00   N-terminal acetlytransferase-related
Trypanosoma brucei gambiense Tbg972.10.6890   N-acetyltransferase subunit Nat1, putative
Trypanosoma brucei Tb927.10.5670   N-acetyltransferase subunit Nat1, putative
Trypanosoma congolense TcIL3000_10_4810   N-acetyltransferase subunit Nat1, putative
Trypanosoma cruzi TcCLB.510301.80   N-acetyltransferase subunit Nat1, putative
Trypanosoma cruzi TcCLB.504163.110   N-acetyltransferase subunit Nat1, putative
Toxoplasma gondii TGME49_223710   tetratricopeptide repeat-containing protein
Theileria parva TP01_1184   hypothetical protein
Trichomonas vaginalis TVAG_473860   conserved hypothetical protein
Trichomonas vaginalis TVAG_211340   O-linked N-acetylglucosamine transferase, ogt, putative
Trichomonas vaginalis TVAG_437210   conserved hypothetical protein

Essentiality

TGME49_223710 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5670 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5670 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5670 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5670 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y50D7A.4 Caenorhabditis elegans embryonic lethal wormbase
TGME49_223710 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_223710 (Toxoplasma gondii), tetratricopeptide repeat-containing protein
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