Detailed view for TGME49_215130

Basic information

TDR Targets ID: 263789
Toxoplasma gondii, adaptor-related protein complex 3, sigma 2 subunit
Source Database / ID:  ToxoDB 

pI: 4.6881 | Length (AA): 158 | MW (Da): 18004 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01217   Clathrin adaptor complex small chain

Gene Ontology

Mouse over links to read term descriptions.
GO:0030123   AP-3 adaptor complex  
GO:0030117   membrane coat  
GO:0008565   protein transporter activity  
GO:0005515   protein binding  
GO:0016192   vesicle-mediated transport  
GO:0015031   protein transport  
GO:0006886   intracellular protein transport  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 149 1gw5 (S) 7 142 31.00 0 1 1.35 -1.04
1 154 1w63 (Q) 7 147 35.00 0 1 1.3 -0.61
1 153 4p6z (S) 1 147 37.00 0 1 1.53045 -1.38
1 148 2vgl (S) 1 142 32.00 0 1 1.43021 -1.27
2 142 5mc7 (A) 13 147 25.00 0.00000091 1 1.29751 -1.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128281)

Species Accession Gene Product
Arabidopsis thaliana AT3G50860   Clathrin adaptor complex small chain family protein
Brugia malayi Bm1_07400   adaptin or adaptin-related protein protein 8
Candida albicans CaO19.8023   similar to S. cerevisiae APS3 (YJL024C) sigma3-like subunit of the clathrin-associated protein AP-3 complex
Candida albicans CaO19.393   similar to S. cerevisiae APS3 (YJL024C) sigma3-like subunit of the clathrin-associated protein AP-3 complex
Caenorhabditis elegans CELE_Y48G8AL.14   Protein APS-3
Dictyostelium discoideum DDB_G0275405   sigma adaptin
Drosophila melanogaster Dmel_CG3029   orange
Echinococcus granulosus EgrG_001173750   AP 3 complex subunit sigma 2
Echinococcus granulosus EgrG_001113700   AP 3 complex subunit sigma 2
Entamoeba histolytica EHI_103550   clathrin adaptor complex, small chain, putative
Echinococcus multilocularis EmuJ_001173750   AP 3 complex subunit sigma 2
Echinococcus multilocularis EmuJ_001113700   AP 3 complex subunit sigma 2
Homo sapiens 1176   adaptor-related protein complex 3, sigma 1 subunit
Homo sapiens ENSG00000157823   adaptor-related protein complex 3, sigma 2 subunit
Leishmania braziliensis LbrM.24.1320   adaptor complex AP-3 small subunit, putative
Leishmania donovani LdBPK_241610.1   adaptor complex AP-3 small subunit, putative
Leishmania infantum LinJ.24.1610   adaptor complex AP-3 small subunit, putative
Leishmania major LmjF.24.1540   adaptor complex AP-3 small subunit, putative
Leishmania mexicana LmxM.24.1540   adaptor complex AP-3 small subunit, putative
Loa Loa (eye worm) LOAG_08409   adaptin
Mus musculus ENSMUSG00000024480   adaptor-related protein complex 3, sigma 1 subunit
Mus musculus ENSMUSG00000063801   adaptor-related protein complex 3, sigma 2 subunit
Neospora caninum NCLIV_052120   adaptor-related protein complex 3, sigma 2 subunit, putative
Oryza sativa 4332139   Os03g0228400
Oryza sativa 9270690   Os01g0353600
Plasmodium berghei PBANKA_1463400   AP-3 complex subunit sigma, putative
Plasmodium falciparum PF3D7_1250500   AP-3 complex subunit sigma, putative
Plasmodium knowlesi PKNH_1470500   AP-3 complex subunit sigma, putative
Plasmodium vivax PVX_101420   AP-3 complex subunit sigma, putative
Saccharomyces cerevisiae YJL024C   Aps3p
Schistosoma japonicum Sjp_0005740   AP-3 complex subunit sigma-1, putative
Schistosoma mansoni Smp_034400   adaptor-related protein complex
Schmidtea mediterranea mk4.001766.00  
Schmidtea mediterranea mk4.004366.00  
Trypanosoma brucei gambiense Tbg972.8.6860   clathrin assembly sigma-adaptin protein 3, putative
Trypanosoma brucei Tb927.8.6680   clathrin assembly sigma-adaptin protein 3, putative
Trypanosoma congolense TcIL3000_8_6560   clathrin assembly sigma-adaptin protein 3, putative
Trypanosoma cruzi TcCLB.506399.10   sigma-adaptin 3, putative
Trypanosoma cruzi TcCLB.507989.20   sigma-adaptin 3, putative
Trypanosoma cruzi TcCLB.509243.50   sigma-adaptin 3, putative
Trypanosoma cruzi TcCLB.510063.10   sigma-adaptin 3, putative
Toxoplasma gondii TGME49_215130   adaptor-related protein complex 3, sigma 2 subunit

Essentiality

TGME49_215130 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6680 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.6680 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.6680 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.6680 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y48G8AL.14 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y48G8AL.14 Caenorhabditis elegans larval arrest wormbase
CELE_Y48G8AL.14 Caenorhabditis elegans sterile wormbase
PBANKA_1463400 Plasmodium berghei Slow plasmo
TGME49_215130 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus AP-2 complex subunit sigma 142 aa 32.0% 147 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_215130 (Toxoplasma gondii), adaptor-related protein complex 3, sigma 2 subunit
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