Detailed view for TGME49_213550

Basic information

TDR Targets ID: 263817
Toxoplasma gondii, DHHC zinc finger domain-containing protein
Source Database / ID:  ToxoDB 

pI: 6.4776 | Length (AA): 1362 | MW (Da): 146326 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 4

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01529   DHHC palmitoyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
48 146 1xl4 (A) 43 148 10.00 0 0 0.47 -0.2
322 478 2l7n (A) 1044 1202 15.00 0 0.07 0.298572 -0.83
539 1072 1lp3 (A) 95 571 9.00 0 0.02 0.17037 1.66
998 1223 5cwp (A) 1 226 12.00 0.025 0.04 0.359532 -0.91
1038 1103 2dm1 (A) 1 72 39.00 0.13 0.08 0.249358 0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_127158)

Species Accession Gene Product
Arabidopsis thaliana AT3G48760   putative S-acyltransferase
Arabidopsis thaliana AT5G41060   DHHC-type zinc finger family protein
Arabidopsis thaliana AT3G26935   putative S-acyltransferase
Arabidopsis thaliana AT4G24630   DHHC-type zinc finger family protein
Arabidopsis thaliana AT5G50020   putative S-acyltransferase
Babesia bovis BBOV_II002930   DHHC zinc finger domain containing protein
Candida albicans CaO19.4466   protein involved in palmitoylation and localization of RAS2
Candida albicans CaO19.11947   similar to ERF2: RAS2p palmitoylation and localization
Candida albicans CaO19.11946   protein involved in palmitoylation and localization of RAS2
Caenorhabditis elegans CELE_Y47H9C.2   Protein DHHC-2
Cryptosporidium hominis Chro.70184   hypothetical protein
Cryptosporidium parvum cgd7_1560   DHHC family palmitoyl transferase with a signal peptide and 4 transmembrane domains
Dictyostelium discoideum DDB_G0276997   DHHC-type zinc finger-containing protein
Dictyostelium discoideum DDB_G0284603   hypothetical protein
Drosophila melanogaster Dmel_CG42318   approximated
Echinococcus granulosus EgrG_000160400   palmitoyltransferase zdhhc9
Echinococcus granulosus EgrG_002010500   palmitoyltransferase ZDHHC14
Entamoeba histolytica EHI_198560   hypothetical protein, conserved domain containing
Echinococcus multilocularis EmuJ_000160000   palmitoyltransferase ZDHHC14
Echinococcus multilocularis EmuJ_000160400   palmitoyltransferase zdhhc9
Echinococcus multilocularis EmuJ_000161000   palmitoyltransferase ZDHHC14
Giardia lamblia GL50803_6733   Zinc finger domain
Giardia lamblia GL50803_8619   Zinc finger domain
Homo sapiens ENSG00000175048   zinc finger, DHHC-type containing 14
Homo sapiens ENSG00000188706   zinc finger, DHHC-type containing 9
Leishmania braziliensis LbrM.25.1870   hypothetical protein, conserved
Leishmania donovani LdBPK_252390.1   hypothetical protein, conserved
Leishmania infantum LinJ.25.2390   hypothetical protein, conserved
Leishmania major LmjF.25.2280   hypothetical protein, conserved
Leishmania mexicana LmxM.25.2280   hypothetical protein, conserved
Mus musculus ENSMUSG00000034265   zinc finger, DHHC domain containing 14
Mus musculus ENSMUSG00000036985   zinc finger, DHHC domain containing 9
Neospora caninum NCLIV_069490   hypothetical protein
Oryza sativa 4326900   Os01g0279000
Oryza sativa 4346154   Os08g0535400
Oryza sativa 4325051   Os01g0868200
Oryza sativa 4347526   Os09g0508300
Oryza sativa 4338902   Os05g0436900
Oryza sativa 4327233   Os01g0925300
Oryza sativa 4346178   Os08g0539100
Plasmodium berghei PBANKA_1420900   palmitoyltransferase DHHC4, putative
Plasmodium falciparum PF3D7_0714300   palmitoyltransferase DHHC4, putative
Plasmodium knowlesi PKNH_1423200   palmitoyltransferase DHHC4, putative
Plasmodium vivax PVX_122945   palmitoyltransferase, putative
Plasmodium yoelii PY02619   unknown protein
Saccharomyces cerevisiae YLR246W   Erf2p
Schistosoma japonicum Sjp_0061890   Probable palmitoyltransferase ZDHHC8, putative
Schistosoma japonicum Sjp_0023850   Probable palmitoyltransferase ZDHHC5, putative
Schistosoma mansoni Smp_073070   hypothetical protein
Schistosoma mansoni Smp_146700   zinc finger protein
Schmidtea mediterranea mk4.005341.00   Palmitoyltransferase
Schmidtea mediterranea mk4.002530.02  
Schmidtea mediterranea mk4.003021.01   Palmitoyltransferase
Trypanosoma brucei gambiense Tbg972.3.2380   hypothetical protein, conserved
Trypanosoma brucei Tb927.3.2400   palmitoyl acyltransferase 10, putative
Trypanosoma congolense TcIL3000_3_1430   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508479.200   palmitoyl acyltransferase 10, putative
Toxoplasma gondii TGME49_213550   DHHC zinc finger domain-containing protein
Theileria parva TP01_0341   hypothetical protein, conserved
Theileria parva TP03_0623   hypothetical protein
Theileria parva TP04_0300   hypothetical protein

Essentiality

TGME49_213550 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.2400 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.2400 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.2400 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.2400 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1420900 Plasmodium berghei Essential plasmo
TGME49_213550 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_213550 (Toxoplasma gondii), DHHC zinc finger domain-containing protein
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