Detailed view for TGME49_288520

Basic information

TDR Targets ID: 263831
Toxoplasma gondii, ATPase, AAA family protein
Source Database / ID:  ToxoDB 

pI: 5.0873 | Length (AA): 502 | MW (Da): 56101 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)

Gene Ontology

Mouse over links to read term descriptions.
GO:0017111   nucleoside-triphosphatase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
171 406 1xwi (A) 124 347 24.00 0 1 0.63 -0.26
174 407 1ixz (A) 149 372 23.00 0 0.99 0.79 -0.71
3 63 3m65 (A) 149 209 10.00 0.28 0.01 0.251414 -0.45
145 445 4xgu (A) 88 394 39.00 0 1 0.936602 0.36
216 311 3nwj (A) 98 193 18.00 0 1 0.442235 -0.32
217 246 1zp6 (A) 10 39 40.00 0 0.3 0.348761 1.23

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128995)

Species Accession Gene Product
Arabidopsis thaliana AT4G24710   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Brugia malayi Bm1_18485   ATPase, AAA family protein
Caenorhabditis elegans CELE_F10B5.5   Protein PCH-2
Cryptosporidium hominis Chro.60073   thyroid receptor interacting protein 13 (TRIP-13)
Cryptosporidium hominis Chro.60074   protein-related binding protein
Cryptosporidium parvum cgd6_550   Pch2p like AAA ATpase
Dictyostelium discoideum DDB_G0279111   AAA ATPase domain-containing protein
Drosophila melanogaster Dmel_CG31453   CG31453 gene product from transcript CG31453-RB
Echinococcus granulosus EgrG_000823600   pachytene checkpoint protein 2
Echinococcus multilocularis EmuJ_000823600   pachytene checkpoint protein 2
Giardia lamblia GL50803_8524   Transitional endoplasmic reticulum ATPase
Homo sapiens ENSG00000071539   thyroid hormone receptor interactor 13
Leishmania braziliensis LbrM.28.1960   ATPase-like protein
Leishmania donovani LdBPK_281910.1   ATPase-like protein
Leishmania infantum LinJ.28.1910   ATPase-like protein
Leishmania major LmjF.28.1790   ATPase-like protein
Leishmania mexicana LmxM.28.1790   ATPase-like protein
Loa Loa (eye worm) LOAG_11130   hypothetical protein
Mus musculus ENSMUSG00000021569   thyroid hormone receptor interactor 13
Neospora caninum NCLIV_040990   Cell division cycle protein, related
Oryza sativa 4336167   Os04g0479000
Plasmodium berghei PBANKA_0909500   AAA family ATPase, putative
Plasmodium falciparum PF3D7_1139500   AAA family ATPase, putative, unspecified product
Plasmodium knowlesi PKNH_0937400   AAA family ATPase, putative
Plasmodium vivax PVX_092575   AAA family ATPase, putative
Plasmodium yoelii PY02067   ATPase, AAA family, putative
Saccharomyces cerevisiae YBR186W   Pch2p
Schistosoma japonicum Sjp_0028260   ko:K01516 nucleoside-triphosphatase [EC3.6.1.15], putative
Schistosoma mansoni Smp_171260   thyroid hormone receptor interactor
Schmidtea mediterranea mk4.000527.02   Pachytene checkpoint protein 2 homolog
Trypanosoma brucei gambiense Tbg972.11.10240   ATPase-like protein, putative
Trypanosoma brucei Tb927.11.9160   ATPase-like protein, putative
Trypanosoma congolense TcIL3000.11.9520   ATPase-like protein, putative
Trypanosoma cruzi TcCLB.510901.90   ATPase protein, putative
Toxoplasma gondii TGME49_288520   ATPase, AAA family protein
Trichomonas vaginalis TVAG_077100   spermatogenesis associated factor, putative

Essentiality

TGME49_288520 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.0900 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.0900 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.0900 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.0900 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F10B5.5 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0909500 Plasmodium berghei Dispensable plasmo
TGME49_288520 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_288520 (Toxoplasma gondii), ATPase, AAA family protein
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