Detailed view for TGME49_313180

Basic information

TDR Targets ID: 263840
Toxoplasma gondii, cell-cycle-associated protein kinase PRP4, putative
Source Database / ID:  ToxoDB 

pI: 8.9025 | Length (AA): 928 | MW (Da): 106637 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
489 899 2etr (A) 8 405 16.00 0 1 0.39 0.3
524 918 1j1b (A) 35 375 27.00 0 1 0.33 0.27
540 884 2eu9 (A) 137 473 30.00 0 1 0.66 -0.64
56 180 4wik (A) 489 597 30.00 0.03 0.03 0.534098 -2.62
56 330 5lm2 (B) 70 331 14.00 0 0.01 0.440136 -1.33
92 209 4wij (A) 461 588 25.00 0.62 0.02 0.469555 -2.66
102 331 4bne (A) 63 293 13.00 0 0.01 0.472345 -1.78
128 195 5hzp (C) 58 125 13.00 0 0 0.637076 -5.14
535 928 4o2z (A) 22 362 26.00 0 1 0.412569 0.77
547 892 4iir (A) 668 1005 54.00 0 1 0.982245 -0.65
553 921 3mwu (A) 0 352 30.00 0 1 0.258629 1.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128795)

Species Accession Gene Product
Arabidopsis thaliana AT1G13350   Protein kinase superfamily protein
Arabidopsis thaliana AT3G53640   protein kinase family protein
Arabidopsis thaliana AT3G25840   serine/threonine-protein kinase PRP4
Babesia bovis BBOV_IV004470   serine/threonine-protein kinase, putative
Brugia malayi Bm1_12230   Protein kinase domain containing protein
Caenorhabditis elegans CELE_F22D6.5   Protein PRPF-4, isoform B
Cryptosporidium hominis Chro.80590   hypothetical protein
Cryptosporidium parvum cgd8_5180   hypothetical protein
Dictyostelium discoideum DDB_G0279703   DYRK family protein kinase
Drosophila melanogaster Dmel_CG7028   CG7028 gene product from transcript CG7028-RE
Echinococcus granulosus EgrG_000670000   Serine:threonine protein kinase PRP4
Entamoeba histolytica EHI_087600   protein kinase, putative
Echinococcus multilocularis EmuJ_000670000   Serine:threonine protein kinase PRP4
Homo sapiens ENSG00000112739   pre-mRNA processing factor 4B
Loa Loa (eye worm) LOAG_10875   CMGC/DYRK/PRP4 protein kinase
Mus musculus ENSMUSG00000021413   PRP4 pre-mRNA processing factor 4 homolog B (yeast)
Neospora caninum NCLIV_056510   Homeodomain interacting protein kinase 3, related
Oryza sativa 9269410   Os03g0710100
Oryza sativa 4352925   Os12g0640800
Plasmodium berghei PBANKA_0933000   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_1114700   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_0912200   protein kinase, putative
Plasmodium vivax PVX_091375   serine/threonine-protein kinase PRP4K, putative
Plasmodium yoelii PY02926   serine/threonine-protein kinase prp4 homolog
Schistosoma japonicum Sjp_0066800   ko:K08827 pre-mRNA-processing factor 4, putative
Schistosoma mansoni Smp_068960.2   serine/threonine protein kinase
Schistosoma mansoni Smp_068960.1   serine/threonine protein kinase
Schmidtea mediterranea mk4.000114.17   Serine/threonine kinase
Schmidtea mediterranea mk4.000114.16   Serine/threonine kinase
Toxoplasma gondii TGME49_313180   cell-cycle-associated protein kinase PRP4, putative
Theileria parva TP01_0303   hypothetical protein
Trichomonas vaginalis TVAG_075270   CMGC family protein kinase

Essentiality

TGME49_313180 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_F22D6.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F22D6.5 Caenorhabditis elegans larval arrest wormbase
CELE_F22D6.5 Caenorhabditis elegans slow growth wormbase
CELE_F22D6.5 Caenorhabditis elegans sterile wormbase
PBANKA_0933000 Plasmodium berghei Essential plasmo
TGME49_313180 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens pre-mRNA processing factor 4B Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 31.1% 190 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 28.7% 331 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 8 411 aa 28.9% 336 aa Compounds References
Patiria pectinifera Cdc2 300 aa 28.1% 327 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 26.8% 328 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 27.3% 344 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 28.0% 328 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0037 1 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 1
0.0064 0.3377 0
0.0063 0.7244 0.7244
0.0059 1 1
0.0022 0.5 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0088 0.4477 1
0.0081 1 0.5
0.0056 1 0.5
0.0026 0.5 0.5
0.0036 0.5 0.5
0.0061 0.6883 0.6656
0.0039 0.5 0.5
0.0027 1 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0066 0.3101 0
0.0062 0.6935 0
0.0059 1 1
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0098 0.3242 0.2614
0.0039 0.9485 0.5
0.0008 0.5 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0069 0.3067 1
0.0059 1 1
0.0042 0.5 0.5
0.0018 0.5 0.5

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

52 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_313180 (Toxoplasma gondii), cell-cycle-associated protein kinase PRP4, putative
Title for this comment
Comment