Detailed view for TGME49_248960

Basic information

TDR Targets ID: 263878
Toxoplasma gondii, methyltransferase domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.1057 | Length (AA): 453 | MW (Da): 49367 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08241   Methyltransferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016300   tRNA (uracil) methyltransferase activity  
GO:0008168   methyltransferase activity  
GO:0008152   metabolic process  
GO:0002098   tRNA wobble uridine modification  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 84 3tht (A) 282 358 18.00 0.37 0.01 0.301178 -0.11
110 336 5cm2 (Z) 39 219 33.00 0 1 0.528304 0.43
133 204 2nbi (A) 421 495 35.00 0.097 0.05 0.35274 0.47
148 196 5e24 (E) 352 399 12.00 0 0.01 0.305368 -1.17
185 318 3l8d (A) 57 192 16.00 0.0000035 0.6 0.451006 -0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127630)

Species Accession Gene Product
Arabidopsis thaliana AT1G36310   S-adenosylmethionine-dependent methyltransferase domain-containing protein
Babesia bovis BBOV_I002790   conserved hypothetical protein
Brugia malayi Bm1_17050   hypothetical protein
Candida albicans CaO19.13735   similar to Diptheria toxin resistance protein
Candida albicans CaO19.6378   similar to Diptheria toxin resistance protein
Caenorhabditis elegans CELE_C14B1.10   Protein ALKB-8
Cryptosporidium hominis Chro.70078   hypothetical protein
Cryptosporidium parvum cgd7_630   Ym1014wp-like, Ymb4 methylase
Dictyostelium discoideum DDB_G0292448   hypothetical protein
Drosophila melanogaster Dmel_CG17807   CG17807 gene product from transcript CG17807-RA
Echinococcus granulosus EgrG_001113800   alkylated dna repair protein alkb 8
Entamoeba histolytica EHI_031630   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_001113800   alkylated dna repair protein alkb 8
Giardia lamblia GL50803_7416   Methyltransferase, putative
Homo sapiens ENSG00000137760   alkB, alkylation repair homolog 8 (E. coli)
Leishmania braziliensis LbrM.35.2860   hypothetical protein, conserved
Leishmania donovani LdBPK_362780.1   methyltransferase domain containing protein, putative
Leishmania infantum LinJ.36.2780   hypothetical protein, conserved
Leishmania major LmjF.36.2650   hypothetical protein, conserved
Leishmania mexicana LmxM.36.2650   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_09472   hypothetical protein
Mus musculus ENSMUSG00000025899   alkB, alkylation repair homolog 8 (E. coli)
Neospora caninum NCLIV_065060   hypothetical protein, conserved
Oryza sativa 4330741   Os02g0750500
Plasmodium berghei PBANKA_0512100   methyltransferase, putative
Plasmodium falciparum PF3D7_1028000   methyltransferase, putative
Plasmodium knowlesi PKNH_0612400   methyltransferase, putative
Plasmodium vivax PVX_111320   hypothetical protein, conserved
Plasmodium yoelii PY02953   hypothetical protein
Saccharomyces cerevisiae YML014W   Trm9p
Schistosoma japonicum Sjp_0000170   ko:K10770 alkylated DNA repair protein alkB homolog 8, putative
Schistosoma mansoni Smp_124160.1   hypothetical protein
Schistosoma mansoni Smp_124160.2   hypothetical protein
Schmidtea mediterranea mk4.007834.04   Alkylated DNA repair protein alkB homolog 8
Schmidtea mediterranea mk4.011803.00   Alkylated DNA repair protein alkB homolog 8
Trypanosoma brucei gambiense Tbg972.10.9260   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0775   methyltransferase domain containing protein, putative
Trypanosoma congolense TcIL3000_10_6520   methyltransferase domain containing protein, putative
Trypanosoma cruzi TcCLB.510105.150   methyltransferase domain containing protein, putative
Trypanosoma cruzi TcCLB.509715.140   methyltransferase domain containing protein, putative
Toxoplasma gondii TGME49_248960   methyltransferase domain-containing protein
Theileria parva TP01_0568   hypothetical protein, conserved
Trichomonas vaginalis TVAG_031080   conserved hypothetical protein
Trichomonas vaginalis TVAG_123080   conserved hypothetical protein

Essentiality

TGME49_248960 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.7560 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.7560 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0512100 Plasmodium berghei Slow plasmo
TGME49_248960 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_248960 (Toxoplasma gondii), methyltransferase domain-containing protein
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