Detailed view for TGME49_211230

Basic information

TDR Targets ID: 263983
Toxoplasma gondii, eukaryotic initiation factor-2B, alpha subunit, putative
Source Database / ID:  ToxoDB 

pI: 5.4864 | Length (AA): 389 | MW (Da): 43457 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01008   Initiation factor 2 subunit family

Gene Ontology

Mouse over links to read term descriptions.
GO:0044237   cellular metabolic process  

Metabolic Pathways

RNA transport (KEGG)

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
200 580 1fnn (A) 3 371 16.00 0 0.84 0.27 0.22
200 417 1jbk (A) 157 351 10.00 0.000000018 0.14 0.11 -0.04
942 1251 1t5o (A) 9 339 19.00 0 1 0.04 -0.15
971 1241 1vb5 (A) 4 272 22.00 0 1 0.13 -0.43
1 165 5cwp (A) 1 163 17.00 0.0017 0.3 0.673965 -0.82
30 387 2yvk (A) 8 348 18.00 0 1 1.02111 0.17
57 387 3a11 (A) 6 311 23.00 0 1 1.0957 -0.17
61 389 3ecs (C) 6 307 34.00 0 1 1.08156 0.26
222 386 1w2w (B) 223 411 21.00 0 0.56 0.303965 0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_127805)

Species Accession Gene Product
Arabidopsis thaliana AT1G53900   eukaryotic translation initiation factor 2B family protein
Arabidopsis thaliana AT1G53880   eukaryotic translation initiation factor 2B-like protein
Arabidopsis thaliana AT1G72340   eukaryotic translation initiation factor 2B domain-containing protein
Brugia malayi Bm1_21890   Initiation factor 2 subunit family protein
Candida albicans CaO19_6904   hypothetical protein
Candida albicans CaO19.6904   similar to S. cerevisiae GCN3 (YKR026C) delta subunit of eIF-2B, the guanine nucleotide exchange factor for translation initiati
Caenorhabditis elegans CELE_ZK1098.4   Protein ZK1098.4
Cryptosporidium hominis Chro.10211   translation initiation factor EIF-2b alpha subunit
Cryptosporidium parvum cgd1_1860   GCN3p like EIF2B,translation initiation factor eIF-2B alpha subunit
Dictyostelium discoideum DDB_G0288961   translation initiation factor eIF-2B alpha subunit
Drosophila melanogaster Dmel_CG7883   CG7883 gene product from transcript CG7883-RA
Echinococcus granulosus EgrG_001069100   translation initiation factor eIF 2B subunit
Entamoeba histolytica EHI_096380   translation initiation factor eIF-2B alpha subunit, putative
Echinococcus multilocularis EmuJ_001069100   translation initiation factor eIF 2B subunit
Giardia lamblia GL50803_91911   Translation initiation factor eIF-2B alpha subunit
Homo sapiens 1967   eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Leishmania braziliensis LbrM.12.0020   translation initiation factor EIF-2b alpha subunit, putative
Leishmania donovani LdBPK_120010.1   translation initiation factor EIF-2b alpha subunit, putative
Leishmania infantum LinJ.12.0001   translation initiation factor EIF-2b alpha subunit, putative
Leishmania major LmjF.12.0010   translation initiation factor EIF-2b alpha subunit, putative
Leishmania mexicana LmxM.12.0010   translation initiation factor EIF-2b alpha subunit, putative
Loa Loa (eye worm) LOAG_08671   initiation factor 2 subunit family protein
Mus musculus ENSMUSG00000029388   eukaryotic translation initiation factor 2B, subunit 1 (alpha)
Neospora caninum NCLIV_011760   hypothetical protein
Oryza sativa 4352258   Os12g0497400
Plasmodium berghei PBANKA_0702200   translation initiation factor eIF-2B subunit alpha, putative
Plasmodium falciparum PF3D7_0828500   translation initiation factor eIF-2B subunit alpha, putative
Plasmodium knowlesi PKNH_1322600   translation initiation factor eIF-2B subunit alpha, putative
Plasmodium vivax PVX_088930   translation initiation factor EIF-2b alpha subunit, putative
Plasmodium yoelii PY07384   Initiation factor 2 subunit family, putative
Saccharomyces cerevisiae YKR026C   translation initiation factor eIF2B subunit alpha
Schistosoma japonicum Sjp_0027200   ko:K03239 translation initiation factor eIF-2B alpha subunit, putative
Schistosoma mansoni Smp_084900   translation initiation factor EIF-2b alpha subunit
Schmidtea mediterranea mk4.007895.01   Probable translation initiation factor eIF-2B subunit alpha
Schmidtea mediterranea mk4.005423.00   Probable translation initiation factor eIF-2B subunit alpha
Trypanosoma brucei gambiense Tbg972.6.990   translation initiation factor EIF-2b alpha subunit, putative,eIF-2B GDP-GTP exchange factor
Trypanosoma brucei Tb927.6.1280   translation initiation factor EIF-2b alpha subunit, putative
Trypanosoma congolense TcIL3000_0_44430   translation initiation factor EIF-2b alpha subunit, putative
Trypanosoma cruzi TcCLB.507603.20   translation initiation factor EIF-2b alpha subunit, putative
Trypanosoma cruzi TcCLB.509429.90   translation initiation factor EIF-2b alpha subunit, putative
Toxoplasma gondii TGME49_211230   eukaryotic initiation factor-2B, alpha subunit, putative
Trichomonas vaginalis TVAG_118970   translation initiation factor eif-2B beta subunit, putative

Essentiality

TGME49_211230 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1280 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1280 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.1280 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1280 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0702200 Plasmodium berghei Slow plasmo
TGME49_211230 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_211230 (Toxoplasma gondii), eukaryotic initiation factor-2B, alpha subunit, putative
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