Detailed view for TGME49_313190

Basic information

TDR Targets ID: 264135
Toxoplasma gondii, Rab18/RabC-family small GTPase
Source Database / ID:  ToxoDB 

pI: 7.3214 | Length (AA): 212 | MW (Da): 23481 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00071   Ras family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0005515   protein binding  
GO:0003924   GTPase activity  
GO:0015031   protein transport  
GO:0007264   small GTPase mediated signal transduction  
GO:0007165   signal transduction  
GO:0006913   nucleocytoplasmic transport  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 202 2bcg (Y) 3 196 44.00 0 1 1.54 -1.71
10 178 3rab (A) 18 186 46.00 0 1 1.51 -2.3
15 184 1x3s (A) 9 178 60.00 0 1 1.71 -2.18
9 202 2bcg (Y) 3 196 43.00 0.00000023 1 1.54369 -1.17
10 177 3rwo (B) 9 176 44.00 0.000012 1 1.47805 -1.64
10 181 1x3s (A) 4 175 59.00 0 1 1.63522 -1.63
17 117 4fmc (F) 14 114 70.00 0 0.76 0.668315 1.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Bradyzoite. Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129466)

Species Accession Gene Product
Arabidopsis thaliana AT5G03530   ras-related protein RABC2a
Arabidopsis thaliana AT1G43890   Ras-related protein Rab-18
Brugia malayi Bm1_16805   Ras-related protein Rab-18A
Caenorhabditis elegans CELE_Y92C3B.3   Protein RAB-18, isoform A
Dictyostelium discoideum DDB_G0289827   Rab GTPase
Echinococcus granulosus EgrG_002011000   Rab GTPase
Echinococcus multilocularis EmuJ_001194700   Rab GTPase
Homo sapiens ENSG00000099246   RAB18, member RAS oncogene family
Loa Loa (eye worm) LOAG_08876   rab family protein 18
Mus musculus 19330   RAB18, member RAS oncogene family
Neospora caninum NCLIV_056520   Rab1-prov protein, related
Oryza sativa 4331607   Os03g0146000
Oryza sativa 4348795   Os10g0456600
Plasmodium berghei PBANKA_1223300   ras-related protein Rab-18, putative
Plasmodium falciparum PF3D7_0807300   ras-related protein Rab-18
Plasmodium knowlesi PKNH_0111500   ras-related protein Rab-18, putative
Plasmodium vivax PVX_088180   ras-related protein Rab-18, putative
Plasmodium yoelii PY01075   Rab18 GTPase-related
Schistosoma japonicum Sjp_0098680   Ras-related protein Rab-18, putative
Schistosoma japonicum Sjp_0054760   Ras-related protein Rab-18, putative
Schistosoma mansoni Smp_008230   rab-18
Schmidtea mediterranea mk4.001996.02   Ras-related protein Rab-18
Schmidtea mediterranea mk4.001897.00   Ras-related protein Rab-18
Schmidtea mediterranea mk4.001769.02   Ras-related protein Rab-18
Toxoplasma gondii TGME49_313190   Rab18/RabC-family small GTPase
Trichomonas vaginalis TVAG_192440   RAC, putative

Essentiality

TGME49_313190 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_Y92C3B.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y92C3B.3 Caenorhabditis elegans larval arrest wormbase
CELE_Y92C3B.3 Caenorhabditis elegans larval lethal wormbase
CELE_Y92C3B.3 Caenorhabditis elegans sterile wormbase
TGME49_313190 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_313190 (Toxoplasma gondii), Rab18/RabC-family small GTPase
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