Detailed view for TGME49_314430

Basic information

TDR Targets ID: 264291
Toxoplasma gondii, serine/threonine specific protein phosphatase
Source Database / ID:  ToxoDB 

pI: 8.5823 | Length (AA): 479 | MW (Da): 53794 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00782   Dual specificity phosphatase, catalytic domain
PF14671   Dual specificity protein phosphatase, N-terminal half

Gene Ontology

Mouse over links to read term descriptions.
GO:0007096   regulation of exit from mitosis  
GO:0008138   protein tyrosine/serine/threonine phosphatase activity  
GO:0004725   protein tyrosine phosphatase activity  
GO:0004721   phosphoprotein phosphatase activity  
GO:0016791   phosphoric monoester hydrolase activity  
GO:0016311   dephosphorylation  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 359 1ohe (A) 46 380 43.00 0 1 1.39 -1.83
23 331 1ohe (A) 52 360 45.00 0 1 1.46 -2.13
186 437 1d5r (A) 24 328 21.00 0 0.9 0.58 0.45
18 352 1ohe (A) 47 378 42.00 0 1 1.32117 -1.22
186 372 3awf (A) 263 460 20.00 0 1 0.556197 -0.37
191 335 3s4o (A) 17 162 25.00 0 1 0.622514 -1.41
225 363 2esb (A) 43 176 22.00 0 0.53 0.422988 -0.01
265 296 2hc1 (A) 1875 1915 56.00 0.94 0.91 0.340306 0.84
325 453 4k6j (A) 649 787 12.00 0 0.02 0.389111 -0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_127827)

Species Accession Gene Product
Brugia malayi Bm1_09400   Protein-tyrosine phosphatase containing protein
Candida albicans CaO19.11669   similar to S. cerevisiae CDC14 (YFR028C) protein phosphatase involved in exit from mitosis
Candida albicans CaO19.4192   similar to S. cerevisiae CDC14 (YFR028C) protein phosphatase involved in exit from mitosis
Caenorhabditis elegans CELE_C17G10.4   Protein CDC-14, isoform C
Cryptosporidium hominis Chro.70494   CDC14 A isoform 2
Cryptosporidium parvum cgd7_4470   CDC14 phosphatase
Dictyostelium discoideum DDB_G0272662   hypothetical protein
Drosophila melanogaster Dmel_CG7134   CG7134 gene product from transcript CG7134-RD
Echinococcus granulosus EgrG_000585900   dual specificity protein phosphatase CDC14A
Echinococcus multilocularis EmuJ_000585900   dual specificity protein phosphatase CDC14A
Giardia lamblia GL50803_9270   Dual specificity protein phosphatase CDC14A
Homo sapiens ENSG00000081377   cell division cycle 14B
Homo sapiens ENSG00000079335   cell division cycle 14A
Leishmania braziliensis LbrM.09.0430   tyrosine phosphatase, putative
Leishmania donovani LdBPK_090470.1   tyrosine phosphatase, putative
Leishmania infantum LinJ.09.0470   tyrosine phosphatase, putative
Leishmania major LmjF.09.0420   tyrosine phosphatase, putative
Leishmania mexicana LmxM.09.0420   tyrosine phosphatase, putative
Loa Loa (eye worm) LOAG_06358   hypothetical protein
Mus musculus ENSMUSG00000033102   CDC14 cell division cycle 14B
Mus musculus ENSMUSG00000033502   CDC14 cell division cycle 14A
Neospora caninum NCLIV_057500   dual specificity protein phosphatase CDC14A, putative
Saccharomyces cerevisiae YFR028C   phosphoprotein phosphatase CDC14
Schistosoma japonicum Sjp_0015270   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma japonicum Sjp_0312090   Dual specificity protein phosphatase CDC14A, putative
Schistosoma mansoni Smp_171590   dual specificity protein phosphatase cdc14
Schmidtea mediterranea mk4.000516.00   Probable tyrosine-protein phosphatase cdc-14
Schmidtea mediterranea mk4.011425.00   Probable tyrosine-protein phosphatase cdc-14
Schmidtea mediterranea mk4.002985.00   Probable tyrosine-protein phosphatase cdc-14
Schmidtea mediterranea mk4.011425.01   Probable tyrosine-protein phosphatase cdc-14
Schmidtea mediterranea mk4.006312.00   Probable tyrosine-protein phosphatase cdc-14
Schmidtea mediterranea mk4.021415.00   Probable tyrosine-protein phosphatase cdc-14
Schmidtea mediterranea mk4.073960.00  
Trypanosoma brucei gambiense Tbg972.11.13900   tyrosine phosphatase, putative
Trypanosoma brucei Tb927.11.12430   cell division cycle phosphatase 14, putative
Trypanosoma congolense TcIL3000_0_20340   cell division cycle phosphatase 14, putative
Trypanosoma cruzi TcCLB.503657.70   tyrosine phosphatase, putative
Trypanosoma cruzi TcCLB.511127.340   tyrosine phosphatase, putative
Toxoplasma gondii TGME49_314430   serine/threonine specific protein phosphatase
Trichomonas vaginalis TVAG_458950   tyrosine specific protein phosphatase and dual specificity protein phosphatase, putative
Trichomonas vaginalis TVAG_362610   tyrosine specific protein phosphatase and dual specificity protein phosphatase, putative
Trichomonas vaginalis TVAG_479730   dual specificity protein phosphatase CDC14, putative
Trichomonas vaginalis TVAG_168700   dual specificity protein phosphatase CDC-14 alpha, putative

Essentiality

TGME49_314430 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.4270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.4270 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.4270 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.4270 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C17G10.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C17G10.4 Caenorhabditis elegans slow growth wormbase
YFR028C Saccharomyces cerevisiae inviable yeastgenome
TGME49_314430 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens cell division cycle 14A Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

7 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_314430 (Toxoplasma gondii), serine/threonine specific protein phosphatase
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