Detailed view for TGME49_205510

Basic information

TDR Targets ID: 264455
Toxoplasma gondii, nucleolar protein 5, putative
Source Database / ID:  ToxoDB 

pI: 9.5998 | Length (AA): 490 | MW (Da): 54349 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01798   snoRNA binding domain, fibrillarin
PF08156   NOP5NT (NUC127) domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
218 397 1nt2 (B) 83 265 26.00 0 0.98 0.69 -0.7
4 383 5gip (A) 6 358 34.00 0 1 1.01901 0.67
68 404 5gip (A) 33 376 34.00 0 1 1.02736 0.39
275 426 3gqu (A) 243 370 38.00 0 0.76 0.324704 0.62
389 490 4tql (A) 71 174 13.00 0.083 0 0.338763 -0.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127592)

Species Accession Gene Product
Arabidopsis thaliana AT5G27120   putative SAR DNA-binding protein
Arabidopsis thaliana AT3G05060   putative SAR DNA-binding protein
Babesia bovis BBOV_II006040   nucleolar protein NOP5, putative
Brugia malayi Bm1_49560   NOP5/NOP58
Candida albicans CaO19.8790   similar to S. cerevisiae NOP58 (YOR310C) component of Box C/D snoRNPs
Candida albicans CaO19.1199   similar to S. cerevisiae NOP58 (YOR310C) component of Box C/D snoRNPs
Caenorhabditis elegans CELE_W01B11.3   Protein NOL-5
Cryptosporidium hominis Chro.30248   snoRNA binding domain
Cryptosporidium parvum cgd3_2110   nucleolar protein NOP5/NOP58-like pre-mRNA splicinig factor prp31
Dictyostelium discoideum DDB_G0268098   hypothetical protein
Drosophila melanogaster Dmel_CG10206   CG10206 gene product from transcript CG10206-RA
Echinococcus granulosus EgrG_000897200   Nucleolar protein 5
Entamoeba histolytica EHI_183900   snoRNA binding protein, putative
Echinococcus multilocularis EmuJ_000897200   Nucleolar protein 5
Giardia lamblia GL50803_5359   Nucleolar protein NOP5
Homo sapiens ENSG00000055044   NOP58 ribonucleoprotein
Leishmania braziliensis LbrM.15.1380   nucleolar RNA binding protein, putative
Leishmania donovani LdBPK_151430.1   nucleolar RNA binding protein, putative
Leishmania infantum LinJ.15.1430   nucleolar RNA binding protein, putative
Leishmania major LmjF.15.1380   nucleolar RNA binding protein, putative
Leishmania mexicana LmxM.15.1380   nucleolar RNA binding protein, putative
Loa Loa (eye worm) LOAG_09461   hypothetical protein
Loa Loa (eye worm) LOAG_12523   NOP5/NOP58
Mus musculus ENSMUSG00000026020   NOP58 ribonucleoprotein
Neospora caninum NCLIV_020180   hypothetical protein
Oryza sativa 4332841   Os03g0350300
Oryza sativa 4332840   Os03g0350100
Plasmodium berghei PBANKA_1207000   nucleolar protein 5, putative
Plasmodium falciparum PF3D7_1008800   nucleolar protein 5, putative
Plasmodium knowlesi PKNH_0808200   nucleolar protein 5, putative
Plasmodium vivax PVX_094640   nucleolar protein 5, putative
Plasmodium yoelii PY05710   Putative snoRNA binding domain, putative
Plasmodium yoelii PY01565   Putative snoRNA binding domain, putative
Saccharomyces cerevisiae YOR310C   RNA-processing protein NOP58
Schistosoma japonicum Sjp_0208100   Nucleolar protein 5, putative
Schistosoma mansoni Smp_148990   nucleolar protein NOP56
Schmidtea mediterranea mk4.010250.00   Nucleolar protein 58
Schmidtea mediterranea mk4.032425.00   Nucleolar protein 58
Trypanosoma brucei gambiense Tbg972.9.2810   nucleolar RNA binding protein, putative
Trypanosoma brucei Tb927.9.5320   nucleolar RNA binding protein, putative
Trypanosoma congolense TcIL3000_9_1780   nucleolar RNA binding protein, putative
Trypanosoma cruzi TcCLB.508277.230   nucleolar RNA binding protein, putative
Toxoplasma gondii TGME49_205510   nucleolar protein 5, putative
Theileria parva TP02_0550   nuclear protein, putative
Trichomonas vaginalis TVAG_459140   prp31, putative

Essentiality

TGME49_205510 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_W01B11.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_W01B11.3 Caenorhabditis elegans larval arrest wormbase
CELE_W01B11.3 Caenorhabditis elegans slow growth wormbase
CELE_W01B11.3 Caenorhabditis elegans sterile wormbase
YOR310C Saccharomyces cerevisiae inviable yeastgenome
TGME49_205510 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_205510 (Toxoplasma gondii), nucleolar protein 5, putative
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