Detailed view for TGME49_205590

Basic information

TDR Targets ID: 264843
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 6.924 | Length (AA): 547 | MW (Da): 64944 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0035735   GO:intraciliary transport involved in cilium assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
73 246 4uos (A) 9 183 26.00 0.011 1 0.818399 -2.07
218 400 4uos (A) 1 185 9.00 0 0.21 0.561152 -2.35
260 392 5hmo (A) 805 923 35.00 0.046 0.03 0.398444 0.11

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_131460)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG1625   dilatory
Echinococcus granulosus EgrG_000423500   5 azacytidine induced protein 1
Echinococcus multilocularis EmuJ_000423500   5 azacytidine induced protein 1
Homo sapiens ENSG00000141577   centrosomal protein 131kDa
Leishmania braziliensis LbrM.34.1560   hypothetical protein, conserved
Leishmania donovani LdBPK_351650.1   hypothetical protein, conserved
Leishmania infantum LinJ.35.1650   hypothetical protein, conserved
Leishmania major LmjF.35.1650   hypothetical protein, conserved
Leishmania mexicana LmxM.34.1650   hypothetical protein, conserved
Mus musculus ENSMUSG00000039781   centrosomal protein 131
Neospora caninum NCLIV_020060   hypothetical protein, conserved
Schistosoma japonicum Sjp_0097650   Conserved hypothetical protein
Schistosoma mansoni Smp_103420   hypothetical protein
Schmidtea mediterranea mk4.001659.02  
Schmidtea mediterranea mk4.001659.03  
Schmidtea mediterranea mk4.001659.04  
Trypanosoma brucei gambiense Tbg972.9.9010   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.14300   5-azacytidine-induced protein 1, putative
Trypanosoma cruzi TcCLB.510761.4   hypothetical protein, conserved
Toxoplasma gondii TGME49_205590   hypothetical protein
Trichomonas vaginalis TVAG_027680   conserved hypothetical protein
Trichomonas vaginalis TVAG_438880   DNA double-strand break repair Rad50 ATPase, putative

Essentiality

TGME49_205590 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.4830 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.4830 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.4830 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.4830 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_205590 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_205590 (Toxoplasma gondii), hypothetical protein
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