Detailed view for TGME49_204280

Basic information

TDR Targets ID: 264888
Toxoplasma gondii, cell-cycle-associated protein kinase DYRK, putative
Source Database / ID:  ToxoDB 

pI: 5.9481 | Length (AA): 1180 | MW (Da): 128810 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
580 1057 2acx (A) 25 511 14.00 0 0.81 0.49 0.48
723 1151 2eu9 (A) 138 480 24.00 0 1 0.32 -0.18
1002 1145 1ob3 (A) 166 286 40.00 0.000000034 0.68 0.39 -0.67
670 1172 3kvw (A) 77 463 29.00 0 1 0.401971 0.76
852 892 2r5t (A) 203 244 56.00 0.021 0.53 0.502946 1.09
1054 1178 3gp0 (A) 191 315 15.00 0 0.08 0.294632 -0.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_130162)

Species Accession Gene Product
Arabidopsis thaliana AT3G17750   protein kinase family protein
Arabidopsis thaliana AT1G73460   putative serine/threonine kinase
Arabidopsis thaliana AT1G73450   putative protein kinase
Arabidopsis thaliana AT2G40120   putative serine/threonine protein kinase
Babesia bovis BBOV_III009650   protein kinase domain containing protein
Cryptosporidium hominis Chro.70343   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase TbPK4
Cryptosporidium parvum cgd7_3050   protein kinase
Giardia lamblia GL50803_17558   Kinase, CMGC DYRK
Giardia lamblia GL50803_137695   Kinase, CMGC DYRK
Leishmania braziliensis LbrM.14.1060   protein kinase, putative
Leishmania braziliensis LbrM.21.1940   protein kinase, putative
Leishmania donovani LdBPK_212010.1   protein kinase, putative
Leishmania donovani LdBPK_141140.1   protein kinase, putative
Leishmania infantum LinJ.14.1140   protein kinase, putative
Leishmania infantum LinJ.21.2010   protein kinase, putative
Leishmania major LmjF.21.1650   protein kinase, putative
Leishmania major LmjF.14.1070   protein kinase, putative
Leishmania mexicana LmxM.14.1070   protein kinase, putative
Leishmania mexicana LmxM.21.1650   protein kinase, putative
Neospora caninum NCLIV_020950   CMGC kinase, Dyrk family, putative
Oryza sativa 4327402   Os01g0832900
Oryza sativa 4333928   Os03g0719500
Oryza sativa 4339052   Os05g0466900
Trypanosoma brucei gambiense Tbg972.10.280   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.7.4330   protein kinase, putative
Trypanosoma brucei Tb927.10.350   protein kinase PK4, putative
Trypanosoma brucei Tb927.7.3880   Basal body protein
Trypanosoma congolense TcIL3000_10_210   protein kinase, putative
Trypanosoma cruzi TcCLB.510519.40   protein kinase PK4, putative
Trypanosoma cruzi TcCLB.506869.60   protein kinase PK4, putative
Trypanosoma cruzi TcCLB.511249.60   CMGC/DYRK protein kinase, putative
Toxoplasma gondii TGME49_204280   cell-cycle-associated protein kinase DYRK, putative
Theileria parva TP04_0880   protein kinase, putative
Trichomonas vaginalis TVAG_000200   CMGC family protein kinase
Trichomonas vaginalis TVAG_396210   CMGC family protein kinase
Trichomonas vaginalis TVAG_048130   CMGC family protein kinase
Trichomonas vaginalis TVAG_238860   CMGC family protein kinase
Trichomonas vaginalis TVAG_362360   CMGC family protein kinase

Essentiality

TGME49_204280 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3880 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.3880 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.3880 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.3880 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.350 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.350 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_204280 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0033 1 1
0.0091 1 0.5
0.0064 0.3377 0
0.0022 0.5 0.5
0.0081 1 0.5
0.0063 0.7244 0.7244
0.0056 1 0.5
0.0059 1 1
0.0016 0.5 0.5
0.0032 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.2614
0.0039 0.9485 0.5
0.0059 1 1
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0012 0.5 0.5
0.0027 1 0.5
0.0093 0.8828 0
0.0063 1 1
0.0033 0.5 0.5
0.0012 0.5 0.5
0.0081 0.5 0.5
0.0003 0.5 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0067 0.5 0.5
0.0036 0.5 0.5
0.0062 0.6935 0
0.0061 0.6883 0.6656
0.0007 0.5 0.5
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0069 0.3067 1
0.0029 0.5 0.5
0.0088 0.4477 1
0.0016 0.5 0.5
0.0023 0.5 0.5
0.0037 1 0.5
0.0092 1 0.5
0.0018 0.5 0.5
0.0004 0.5 0.5
0.0026 0.5 0.5
0.0066 0.3101 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

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Gene identifier TGME49_204280 (Toxoplasma gondii), cell-cycle-associated protein kinase DYRK, putative
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