Detailed view for TGME49_221450

Basic information

TDR Targets ID: 264928
Toxoplasma gondii, SPRY domain-containing protein
Source Database / ID:  ToxoDB 

pI: 9.2721 | Length (AA): 521 | MW (Da): 56595 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0051568   histone H3-K4 methylation  
GO:0048188   COMPASS complex  
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 205 4n5a (A) 307 509 12.00 0 0.01 0.471255 -0.28
277 515 5f6l (B) 287 503 28.00 0 0.98 0.381433 0.51
277 515 3toj (A) 287 503 31.00 0 0.83 0.411433 0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128823)

Species Accession Gene Product
Arabidopsis thaliana AT1G51450   protein TRAUCO
Brugia malayi Bm1_16295   SPRY domain containing protein
Candida albicans CaO19.11446   similar to S. pombe ash2 (SPBC13G1.08c) and to S. cerevisiae BRE2 (YLR015W) component of COMPASS histone methylation complex
Candida albicans CaO19.3964   similar to S. pombe ash2 (SPBC13G1.08c) and to S. cerevisiae BRE2 (YLR015W) component of COMPASS histone methylation complex
Caenorhabditis elegans CELE_Y17G7B.2   Protein ASH-2, isoform B
Cryptosporidium hominis Chro.10092   similar to ash2
Cryptosporidium parvum cgd1_740   hypothetical protein
Drosophila melanogaster Dmel_CG6677   absent, small, or homeotic discs 2
Echinococcus granulosus EgrG_000489600   set1:ash2 histone methyltransferase complex
Echinococcus multilocularis EmuJ_000489600   set1:ash2 histone methyltransferase complex
Homo sapiens ENSG00000129691   ash2 (absent, small, or homeotic)-like (Drosophila)
Loa Loa (eye worm) LOAG_02488   SPRY domain-containing protein
Loa Loa (eye worm) LOAG_12574   SPRY domain-containing protein
Mus musculus ENSMUSG00000031575   ash2 (absent, small, or homeotic)-like (Drosophila)
Neospora caninum NCLIV_004990   SPRY domain containing protein, related
Oryza sativa 4349756   Os11g0145500
Oryza sativa 9268228   Os11g0145600
Oryza sativa 4349761   Os11g0146500
Oryza sativa 4351479   Os12g0143200
Plasmodium berghei PBANKA_0704300   SPRY domain, putative
Plasmodium falciparum PF3D7_0826300   SPRY domain, putative
Plasmodium knowlesi PKNH_1320500   SPRY domain, putative
Plasmodium vivax PVX_089045   hypothetical protein, conserved
Plasmodium yoelii PY05573   similar to ash2
Saccharomyces cerevisiae YLR015W   Bre2p
Schistosoma japonicum Sjp_0100120   Set1/Ash2 histone methyltransferase complex subunit ASH2, putative
Schistosoma mansoni Smp_142170   trithorax protein ash2
Schmidtea mediterranea mk4.001515.11   Set1/Ash2 histone methyltransferase complex subunit ASH2
Toxoplasma gondii TGME49_221450   SPRY domain-containing protein
Theileria parva TP03_0423   hypothetical protein
Trichomonas vaginalis TVAG_127960   conserved hypothetical protein
Trichomonas vaginalis TVAG_499590   conserved hypothetical protein
Trichomonas vaginalis TVAG_282630   conserved hypothetical protein

Essentiality

TGME49_221450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_Y17G7B.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y17G7B.2 Caenorhabditis elegans larval arrest wormbase
TGME49_221450 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ash2 (absent, small, or homeotic)-like (Drosophila) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_221450 (Toxoplasma gondii), SPRY domain-containing protein
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