Detailed view for LmjF.36.6090

Basic information

TDR Targets ID: 26494
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0232 | Length (AA): 532 | MW (Da): 60878 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03109   ABC1 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
163 525 1om1 (A) 33 326 15.00 0 0.7 0.6 0.29
172 461 1jpa (A) 632 878 15.00 0.0000000000016 0.91 0.35 0.69
258 463 1u46 (A) 177 377 12.00 0.00000008 0.4 0.46 -0.21
99 484 4ped (A) 259 644 17.00 0.000000000006 1 1.01796 -0.83
162 359 3is5 (A) 132 286 28.00 0.14 0.46 0.31698 1.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128411)

Species Accession Gene Product
Arabidopsis thaliana AT1G61640   ABC1 domain-containing kinase
Arabidopsis thaliana AT1G11390   protein kinase-like protein ABC1K10
Brugia malayi Bm1_21555   hypothetical protein
Candida albicans CaO19.4575   similar to S. cerevisiae YPL109C
Candida albicans CaO19.12044   similar to S. cerevisiae YPL109C
Caenorhabditis elegans CELE_Y32H12A.7   Protein Y32H12A.7
Dictyostelium discoideum DDB_G0267774   ABC1 family protein kinase
Escherichia coli b3835   regulator of octaprenylphenol hydroxylation, ubiquinone synthesis
Homo sapiens ENSG00000133597   aarF domain containing kinase 2
Leishmania braziliensis LbrM.10.0740   hypothetical protein, conserved
Leishmania braziliensis LbrM.35.6400   hypothetical protein, conserved
Leishmania donovani LdBPK_366350.1   ABC1 family, putative
Leishmania donovani LdBPK_100650.1   ABC1 family, putative
Leishmania infantum LinJ.36.6350   hypothetical protein, conserved
Leishmania infantum LinJ.10.0650   hypothetical protein, conserved
Leishmania major LmjF.10.0600   hypothetical protein, conserved
Leishmania major LmjF.36.6090   hypothetical protein, conserved
Leishmania mexicana LmxM.10.0600   hypothetical protein, conserved
Leishmania mexicana LmxM.36.6090   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00021   atypical/ABC1/ABC1-C protein kinase
Mus musculus ENSMUSG00000046947   aarF domain containing kinase 2
Oryza sativa 4343579   Os07g0558000
Oryza sativa 4337281   Os04g0660200
Onchocerca volvulus OVOC7827  
Saccharomyces cerevisiae YPL109C   hypothetical protein
Schmidtea mediterranea mk4.059115.00  
Trypanosoma brucei gambiense Tbg972.8.3740   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.10.10590   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.8730   ABC1 family, putative
Trypanosoma brucei Tb927.8.4000   ABC1 family, putative
Trypanosoma congolense TcIL3000_10_7520   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_0_22430   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507623.90   ABC1 family, putative
Trypanosoma cruzi TcCLB.505941.30   ABC1 family, putative
Trypanosoma cruzi TcCLB.509789.10   ABC1 family, putative

Essentiality

LmjF.36.6090 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.8730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.8730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.8730 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.8730 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.4000 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.4000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.4000 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.4000 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3835 Escherichia coli essential goodall
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.36.6090 (Leishmania major), hypothetical protein, conserved
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