Detailed view for TGME49_226240

Basic information

TDR Targets ID: 265018
Toxoplasma gondii, bud site selection protein, putative
Source Database / ID:  ToxoDB 

pI: 10.707 | Length (AA): 198 | MW (Da): 23202 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12171   Zinc-finger double-stranded RNA-binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0008270   zinc ion binding  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
42 101 1zr9 (A) 29 88 45.00 0 0.91 1.1 0.17
34 126 1o6d (A) 2 95 31.00 0.34 0.06 0.632297 0.46
79 165 4s28 (A) 494 581 17.00 0.000052 0.15 0.591694 -0.24
137 197 1zr9 (A) 28 88 44.00 0 0.99 0.749381 0.91
148 179 2em4 (A) 9 40 25.00 0.67 0.39 0.355916 0.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128822)

Species Accession Gene Product
Arabidopsis thaliana AT2G36930   C2H2-type zinc finger-containing protein
Babesia bovis BBOV_II001440   hypothetical protein
Brugia malayi Bm1_39315   Zinc finger, C2H2 type family protein
Candida albicans CaO19.10451   similar to protein that copurifies with nuclear pore complex
Candida albicans CaO19.2934   similar to protein that copurifies with nuclear pore complex
Caenorhabditis elegans CELE_Y56A3A.18   Protein Y56A3A.18
Dictyostelium discoideum DDB_G0275351   hypothetical protein
Drosophila melanogaster Dmel_CG3224   CG3224 gene product from transcript CG3224-RA
Echinococcus granulosus EgrG_000777500   zinc finger C2H2 type
Entamoeba histolytica EHI_017720   zinc finger protein, putative
Echinococcus multilocularis EmuJ_000777500   zinc finger, C2H2 type
Giardia lamblia GL50803_16877   Zinc finger domain
Homo sapiens 51042   zinc finger protein 593
Loa Loa (eye worm) LOAG_11000   zinc finger protein
Mus musculus ENSMUSG00000028840   zinc finger protein 593
Neospora caninum NCLIV_046700   zinc finger protein, putative
Oryza sativa 4346346   Os08g0564500
Onchocerca volvulus OVOC4965   Zinc finger protein 593 homolog
Plasmodium berghei PBANKA_1327800   zinc finger protein, putative
Plasmodium falciparum PF3D7_1464400   zinc finger protein, putative
Plasmodium knowlesi PKNH_1217100   zinc finger protein, putative
Plasmodium vivax PVX_117245   hypothetical protein, conserved
Saccharomyces cerevisiae YLR074C   Bud20p
Schistosoma japonicum Sjp_0054090   Bud site selection protein 20, putative
Schistosoma mansoni Smp_070130   zinc finger protein
Schmidtea mediterranea mk4.000134.13   Zinc finger protein 593
Toxoplasma gondii TGME49_226240   bud site selection protein, putative

Essentiality

TGME49_226240 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_Y56A3A.18 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y56A3A.18 Caenorhabditis elegans larval arrest wormbase
CELE_Y56A3A.18 Caenorhabditis elegans slow growth wormbase
TGME49_226240 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_226240 (Toxoplasma gondii), bud site selection protein, putative
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