Detailed view for TGME49_237200

Basic information

TDR Targets ID: 265065
Toxoplasma gondii, sphingolipid delta 4 desaturase/c-4 hydroxylase protein des2 family protein
Source Database / ID:  ToxoDB 

pI: 6.5438 | Length (AA): 424 | MW (Da): 48341 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 5

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00487   Fatty acid desaturase
PF08557   Sphingolipid Delta4-desaturase (DES)

Gene Ontology

Mouse over links to read term descriptions.
GO:0042284   sphingolipid delta-4 desaturase activity  
GO:0030148   sphingolipid biosynthetic process  
GO:0016021   integral to membrane  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  
GO:0006633   fatty acid biosynthetic process  
GO:0006629   lipid metabolic process  

Metabolic Pathways

Sphingolipid metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
327 372 2oz8 (A) 66 117 35.00 0.74 0.42 0.434991 0.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128204)

Species Accession Gene Product
Arabidopsis thaliana AT4G04930   sphingolipid delta-4 desaturase
Brugia malayi Bm1_54630   Fatty acid desaturase family protein
Candida albicans CaO19.11530   sphingolipid delta 4 desaturase similar to S. pombe dsd1 (SPBC3B8.07c)
Candida albicans CaO19.4048   sphingolipid delta 4 desaturase similar to S. pombe dsd1 (SPBC3B8.07c)
Caenorhabditis elegans CELE_Y54E5A.1   Protein TTM-5
Caenorhabditis elegans CELE_F33D4.4   Protein F33D4.4
Dictyostelium discoideum DDB_G0269738   dihydroceramide desaturase
Drosophila melanogaster Dmel_CG9078   infertile crescent
Echinococcus granulosus EgrG_002000500   Fatty acid desaturase type 1
Echinococcus multilocularis EmuJ_000357100   Peptidase M, neutral zinc metallopeptidases, zinc binding site
Echinococcus multilocularis EmuJ_000346400   Sphingolipid delta(4) desaturase DES1
Echinococcus multilocularis EmuJ_000357200   Peptidase M, neutral zinc metallopeptidases, zinc binding site
Echinococcus multilocularis EmuJ_000006800   Fatty acid desaturase, type 1
Homo sapiens ENSG00000143753   delta(4)-desaturase, sphingolipid 1
Homo sapiens 123099   delta(4)-desaturase, sphingolipid 2
Leishmania braziliensis LbrM.23.1980   fatty acid desaturase
Leishmania infantum LinJ.26.1680   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Leishmania major LmjF.26.1670   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Leishmania major LmjF.26.1690   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Leishmania major LmjF.26.1700   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Leishmania major LmjF.26.1680   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Leishmania mexicana LmxM.26.1700   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Leishmania mexicana LmxM.26.1670   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Leishmania mexicana LmxM.26.1690  
Loa Loa (eye worm) LOAG_10890   fatty acid desaturase
Mus musculus ENSMUSG00000038633   degenerative spermatocyte homolog 1 (Drosophila)
Mus musculus ENSMUSG00000021263   degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase
Neospora caninum NCLIV_051070   membrane fatty acid desaturase, putative
Oryza sativa 4330104   Os02g0639600
Schistosoma japonicum Sjp_0317130   expressed protein
Schmidtea mediterranea mk4.001493.02   Dihydroceramide desaturase
Schmidtea mediterranea mk4.021730.00  
Schmidtea mediterranea mk4.021680.00   Putative sphingolipid delta(4)-desaturase/C4-hydroxylase
Trypanosoma brucei gambiense Tbg972.6.2790   fatty acid desaturase, putative,sphingolipid delta 4 desaturase, putative
Trypanosoma brucei Tb927.6.3000   sphingolipid delta 4 desaturase, putative
Trypanosoma congolense TcIL3000_6_2510   sphingolipid delta 4 desaturase, putative
Trypanosoma cruzi TcCLB.510565.20   fatty acid desaturase, putative
Toxoplasma gondii TGME49_237200   sphingolipid delta 4 desaturase/c-4 hydroxylase protein des2 family protein

Essentiality

TGME49_237200 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.3000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.3000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.3000 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.3000 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_237200 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens delta(4)-desaturase, sphingolipid 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_237200 (Toxoplasma gondii), sphingolipid delta 4 desaturase/c-4 hydroxylase protein des2 family protein
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