Detailed view for TGME49_268210

Basic information

TDR Targets ID: 265103
Toxoplasma gondii, AGC kinase
Source Database / ID:  ToxoDB 

pI: 5.8849 | Length (AA): 515 | MW (Da): 58001 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
20 581 1omw (A) 96 654 19.00 0 1 0.91 0.7
23 580 1omw (A) 115 667 20.00 0 1 1.05 0.39
104 406 2bfx (A) 87 354 32.00 0 1 0.89 -0.99
4 515 3pvu (A) 159 666 20.00 0 1 1.07417 0.67
30 261 2ivs (B) 718 951 30.00 0.00000032 1 0.567785 0.15
34 338 5lvo (A) 80 356 38.00 0 1 0.974333 -0.4
360 514 4uos (A) 19 172 8.00 0 0 0.489471 -1.75

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_136837)

Species Accession Gene Product
Babesia bovis BBOV_I000170   protein kinase domain containing protein
Cryptosporidium hominis Chro.10297   hypothetical protein
Cryptosporidium parvum cgd1_2630   Ser/Thr protein kinase
Dictyostelium discoideum DDB_G0281471   PDK1 family protein kinase
Neospora caninum NCLIV_037990   AGC kinase, putative
Plasmodium berghei PBANKA_0926400   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_1121900   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_0919600   protein kinase, putative
Plasmodium vivax PVX_091715   3-phosphoinositide dependent protein kinase-1, putative
Plasmodium yoelii PY06967   serine/threonine kinase 13-related
Toxoplasma gondii TGME49_268210   AGC kinase
Theileria parva TP03_0842   protein kinase, putative
Theileria parva TP03_0843   hypothetical protein
Theileria parva TP03_0844   hypothetical protein

Essentiality

TGME49_268210 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_268210 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 29.6% 291 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 29.7% 310 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 22.3% 264 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 24.6% 179 aa Compounds References
Patiria pectinifera Cdc2 300 aa 23.5% 264 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 21.7% 267 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 29.2% 291 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 30.1% 326 aa Compounds References
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 27.8% 291 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0012 0.5 0.5
0.0033 1 1
0.0016 0.5 0.5
0.0007 0.5 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0037 1 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0088 0.4477 1
0.0081 1 0.5
0.0056 1 0.5
0.0032 0.5 0.5
0.0093 0.8828 0
0.0063 0.7244 0.7244
0.0059 1 1
0.0022 0.5 0.5
0.0064 0.3377 0
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0098 0.3242 0.2614
0.0039 0.9485 0.5
0.0008 0.5 0.5
0.0092 1 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0062 0.6935 0
0.0059 1 1
0.0027 1 0.5
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0066 0.3101 0
0.0026 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.6656
0.0036 0.5 0.5
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0069 0.3067 1
0.0059 1 1
0.0033 0.5 0.5
0.0063 1 1
0.0004 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0007 0.5 0.5

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

52 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_268210 (Toxoplasma gondii), AGC kinase
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