Detailed view for TGME49_232380

Basic information

TDR Targets ID: 265125
Toxoplasma gondii, WD domain, G-beta repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 7.3532 | Length (AA): 1230 | MW (Da): 134254 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
569 921 1erj (A) 287 706 18.00 0 1 0.47 -0.43
758 883 1got (B) 89 218 26.00 0.000098 0.79 0.48 -0.87
26 99 4zoz (A) 194 267 23.00 0.00016 0.58 0.337663 -0.05
27 346 2qxv (A) 100 429 15.00 0 0.74 0.326563 0.75
475 537 5hmo (A) 813 877 32.00 0.27 0 0.43932 -1.34
590 923 4zb4 (B) 150 502 10.00 0 1 0.264945 0.48
771 923 4aow (A) 154 313 27.00 0.0000017 0.9 0.48149 -0.23
771 926 3uvk (A) 51 204 29.00 0.000000000012 0.99 0.558929 -0.59
821 879 4l9o (B) 2034 2096 42.00 0.13 0.34 0.318067 0.78
910 1133 4x0j (A) 45 277 13.00 0 0.05 0.251514 -0.13
967 1207 5ica (A) 769 931 29.00 0 0.97 0.015335 0.49
968 1100 2x7g (A) 550 681 33.00 0.65 0.37 0.28423 0.35
1032 1207 5ic9 (C) 797 931 30.00 0.000000000006 0.71 -0.256411 1.55

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127984)

Species Accession Gene Product
Arabidopsis thaliana AT3G21540   transducin/WD40 domain-containing protein
Babesia bovis BBOV_III011180   WD domain, G-beta repeat containing protein
Brugia malayi Bm1_05345   WD-repeat protein 3
Candida albicans CaO19.5106   DOM34-interacting protein
Candida albicans CaO19.12572   DOM34-interacting protein
Caenorhabditis elegans CELE_F13H8.2   Protein F13H8.2
Cryptosporidium hominis Chro.80172   WD repeat-containing protein 3
Cryptosporidium parvum cgd8_1450   11x WD40 repeats containing protein of plant origin
Dictyostelium discoideum DDB_G0282623   U3 small nucleolar ribonucleoprotein
Drosophila melanogaster Dmel_CG8064   CG8064 gene product from transcript CG8064-RA
Echinococcus granulosus EgrG_000694000   WD repeat containing protein 3
Entamoeba histolytica EHI_067830   WD domain containing protein
Echinococcus multilocularis EmuJ_000694000   WD repeat containing protein 3
Giardia lamblia GL50803_16264   WD-40 repeat protein
Homo sapiens 10885   WD repeat domain 3
Leishmania braziliensis LbrM.14.0550   hypothetical protein, conserved
Leishmania donovani LdBPK_140580.1   WD domain, G-beta repeat, putative
Leishmania infantum LinJ.14.0580   hypothetical protein, conserved
Leishmania major LmjF.14.0570   hypothetical protein, conserved
Leishmania mexicana LmxM.14.0570   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05321   hypothetical protein
Loa Loa (eye worm) LOAG_15938   hypothetical protein
Mus musculus ENSMUSG00000033285   WD repeat domain 3
Neospora caninum NCLIV_032360   WD-40 repeat protein, putative
Oryza sativa 4331644   Os03g0151700
Onchocerca volvulus OVOC11408  
Plasmodium berghei PBANKA_1311800   U3 small nucleolar RNA-associated protein 12, putative
Plasmodium falciparum PF3D7_1448000   U3 small nucleolar RNA-associated protein 12, putative
Plasmodium knowlesi PKNH_1234100   U3 small nucleolar RNA-associated protein 12, putative
Plasmodium vivax PVX_118095   WD domain, G-beta repeat domain containing protein
Plasmodium yoelii PY01106   hypothetical protein
Saccharomyces cerevisiae YLR129W   Dip2p
Schistosoma japonicum Sjp_0311180   WD repeat-containing protein 3 homolog, putative
Schistosoma japonicum Sjp_0012000   WD repeat-containing protein 3, putative
Schistosoma japonicum Sjp_0100790   WD repeat-containing protein 3, putative
Schistosoma japonicum Sjp_0011980   WD repeat-containing protein 3, putative
Schistosoma mansoni Smp_136010   hypothetical protein
Schmidtea mediterranea mk4.000597.05  
Schmidtea mediterranea mk4.000597.06  
Trypanosoma brucei gambiense Tbg972.7.4750   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.4220   WD domain, G-beta repeat/Dip2/Utp12 Family, putative
Trypanosoma congolense TcIL3000_7_3400   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511245.50   WD domain, G-beta repeat/Dip2/Utp12 Family, putative
Trypanosoma cruzi TcCLB.506661.110   WD domain, G-beta repeat/Dip2/Utp12 Family, putative
Toxoplasma gondii TGME49_232380   WD domain, G-beta repeat-containing protein
Theileria parva TP02_0738   hypothetical protein
Trichomonas vaginalis TVAG_342320   WD repeat containing protein, putative

Essentiality

TGME49_232380 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4220 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.4220 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4220 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.4220 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F13H8.2 Caenorhabditis elegans slow growth wormbase
CELE_F13H8.2 Caenorhabditis elegans sterile wormbase
YLR129W Saccharomyces cerevisiae inviable yeastgenome
TGME49_232380 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_232380 (Toxoplasma gondii), WD domain, G-beta repeat-containing protein
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