Detailed view for TGME49_267440

Basic information

TDR Targets ID: 265149
Toxoplasma gondii, RING zinc finger protein
Source Database / ID:  ToxoDB 

pI: 6.1552 | Length (AA): 384 | MW (Da): 41492 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13920   Zinc finger, C3HC4 type (RING finger)

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
277 373 4ayc (A) 351 446 16.00 0.032 0.03 0.458904 -0.67
327 377 3eb5 (A) 555 601 34.00 0 0.62 0.427413 0.09
329 379 2ecg (A) 28 74 40.00 0.0084 1 0.397413 0.57
329 370 1bor (A) 9 46 53.00 0.0007 0.83 0.488975 1.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite, ME49 Bradyzoite. Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128926)

Species Accession Gene Product
Arabidopsis thaliana AT3G53410   probable E3 ubiquitin-protein ligase LUL2
Arabidopsis thaliana AT3G06140   probable E3 ubiquitin-protein ligase LUL4
Arabidopsis thaliana AT3G09770   probable E3 ubiquitin-protein ligase LOG2
Arabidopsis thaliana AT5G19080   probable E3 ubiquitin-protein ligase LUL3
Babesia bovis BBOV_IV012000   hypothetical protein
Brugia malayi Bm1_03585   hypothetical protein
Caenorhabditis elegans CELE_C11H1.3   Protein C11H1.3
Cryptosporidium hominis Chro.30158   B1045D11.20
Cryptosporidium parvum cgd3_1260   RING domain protein
Dictyostelium discoideum DDB_G0269704   hypothetical protein
Drosophila melanogaster Dmel_CG9941   CG9941 gene product from transcript CG9941-RA
Giardia lamblia GL50803_11052   Zinc finger domain
Homo sapiens ENSG00000102858   mahogunin ring finger 1, E3 ubiquitin protein ligase
Homo sapiens ENSG00000141576   ring finger protein 157
Loa Loa (eye worm) LOAG_14622   hypothetical protein
Loa Loa (eye worm) LOAG_09645   hypothetical protein
Mus musculus ENSMUSG00000022517   mahogunin, ring finger 1
Mus musculus ENSMUSG00000052949   ring finger protein 157
Neospora caninum NCLIV_038740   hypothetical protein
Oryza sativa 4348266   Os10g0204100
Oryza sativa 4332287   Os03g0254900
Oryza sativa 9266835   Os05g0397650
Onchocerca volvulus OVOC4056  
Plasmodium berghei PBANKA_0311900   RING zinc finger protein, putative
Plasmodium falciparum PF3D7_0215100   RING zinc finger protein, putative
Plasmodium knowlesi PKNH_0405700   RING zinc finger protein, putative
Plasmodium vivax PVX_002780   RING zinc finger protein, putative
Plasmodium yoelii PY00197   hypothetical protein
Schmidtea mediterranea mk4.000361.28  
Toxoplasma gondii TGME49_267440   RING zinc finger protein
Theileria parva TP01_1110   hypothetical protein

Essentiality

TGME49_267440 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_C11H1.3 Caenorhabditis elegans adult lethal wormbase
CELE_C11H1.3 Caenorhabditis elegans slow growth wormbase
PBANKA_0311900 Plasmodium berghei Slow plasmo
TGME49_267440 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_267440 (Toxoplasma gondii), RING zinc finger protein
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