Detailed view for TGME49_207900

Basic information

TDR Targets ID: 265317
Toxoplasma gondii, transcription initiation factor TFIIIB
Source Database / ID:  ToxoDB 

pI: 7.2068 | Length (AA): 813 | MW (Da): 88049 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00382   Transcription factor TFIIB repeat
PF07741   Brf1-like TBP-binding domain
PF08271   TFIIB zinc-binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0017025   TATA-binding protein binding  
GO:0006355   regulation of transcription, DNA-dependent  
GO:0001026   GO:TFIIIB-type transcription factor activity  

GO:0000126   transcription factor TFIIIB complex  
GO:0070897   GO:DNA-templated transcriptional preinitiation complex assembly  

GO:0005667   transcription factor complex  
GO:0005634   nucleus  
GO:0016563   transcription activator activity  
GO:0008270   zinc ion binding  
GO:0005515   protein binding  
GO:0003743   translation initiation factor activity  
GO:0030528   transcription regulator activity  
GO:0006413   translational initiation  
GO:0006383   transcription from RNA polymerase III promoter  
GO:0006352   transcription initiation  
GO:0045941   positive regulation of transcription  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
11 219 1c9b (A) 110 314 21.00 0 1 0.77 -1.47
18 208 1ais (B) 1112 1300 25.00 0 1 0.77 -1.59
123 166 1pft (A) 3 46 34.00 0.0046 0.58 0.368221 0.61
127 294 4bbr (M) 23 214 18.00 0 0.99 0.251842 0.41
200 356 4roc (A) 66 233 25.00 0.0000088 1 0.484912 -0.72
202 489 4roc (A) 66 404 17.00 0 1 0.537444 -0.07
205 398 1ais (B) 1109 1300 23.00 0 1 0.632822 -0.97
663 811 4uos (A) 1 155 12.00 0.17 0.03 0.361372 -1.18
679 740 1ngm (B) 447 506 28.00 0 0.04 0.231461 0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127701)

Species Accession Gene Product
Arabidopsis thaliana AT2G45100   Cyclin/Brf1-like TBP-binding protein
Arabidopsis thaliana AT2G01280   Cyclin/Brf1-like TBP-binding protein
Arabidopsis thaliana AT3G09360   Cyclin/Brf1-like TBP-binding protein
Babesia bovis BBOV_IV002720   transcription factor TFIIB subunit, putative
Brugia malayi Bm1_25435   Transcription factor TFIIB repeat family protein
Candida albicans CaO19.13970   similar to S. cerevisiae BRF1 (YGR246C) component of RNA Pol III transcription factor TFIIIB complex
Candida albicans CaO19.6649   similar to S. cerevisiae BRF1 (YGR246C) component of RNA Pol III transcription factor TFIIIB complex
Caenorhabditis elegans CELE_F45E12.2   Protein BRF-1
Cryptosporidium hominis Chro.50018   transcription factor IIIb subunit
Cryptosporidium parvum cgd5_3540   cyclin domain protein
Dictyostelium discoideum DDB_G0281215   TATA box-binding protein-associated factor, RNA polymerase III, subunit 2
Drosophila melanogaster Dmel_CG31256   CG31256 gene product from transcript CG31256-RA
Echinococcus granulosus EgrG_000229600   transcription factor iiib 90 kda subunit
Entamoeba histolytica EHI_158020   transcription initiation factor IIIB chain BRF, putative
Echinococcus multilocularis EmuJ_000229600   transcription factor iiib 90 kda subunit
Giardia lamblia GL50803_4125   Transcription factor IIIB 70 kDa subunit BRF
Homo sapiens ENSG00000185024   BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Leishmania braziliensis LbrM.25.0390   transcription factor, putative
Leishmania donovani LdBPK_250450.1   transcription factor IIIb, putative
Leishmania infantum LinJ.25.0450   transcription factor, putative
Leishmania major LmjF.25.0440   transcription factor, putative
Leishmania mexicana LmxM.25.0440   transcription factor, putative
Loa Loa (eye worm) LOAG_07165   BRF1 protein
Mus musculus ENSMUSG00000011158   BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
Neospora caninum NCLIV_002820   hypothetical protein
Oryza sativa 4338362   Os05g0305100
Plasmodium berghei PBANKA_1313000   transcription factor IIIb subunit, putative
Plasmodium falciparum PF3D7_1449300   transcription factor IIIb subunit, putative
Plasmodium knowlesi PKNH_1232800   transcription factor IIIb subunit, putative
Plasmodium vivax PVX_118025   transcription factor IIIb subunit, putative
Plasmodium yoelii PY07313   transcription factor iiib 70 kDa subunit
Saccharomyces cerevisiae YGR246C   Brf1p
Schistosoma japonicum Sjp_0094040   similar to Transcription factor IIIB 90 kDa subunit, putative
Schistosoma japonicum Sjp_0094050   expressed protein
Schistosoma mansoni Smp_071570   transcription initiation factor brf1
Schmidtea mediterranea mk4.000180.12   Transcription factor IIIB 90 kDa subunit
Schmidtea mediterranea mk4.005392.00   Transcription factor IIIB 90 kDa subunit
Trypanosoma brucei gambiense Tbg972.11.380   transcription factor, putative,transcription factor IIIb, putative
Trypanosoma brucei Tb927.11.470   TFIIB-related factor BRF1
Trypanosoma congolense TcIL3000.11.410   transcription factor IIIb, putative
Trypanosoma cruzi TcCLB.507093.180   transcription factor IIIb, putative
Toxoplasma gondii TGME49_207900   transcription initiation factor TFIIIB
Theileria parva TP03_0376   transcription factor IIIb subunit, putative
Trichomonas vaginalis TVAG_139840   transcription initiation factor brf1, putative

Essentiality

TGME49_207900 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.03.0670 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0670 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.03.0670 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.03.0670 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F45E12.2 Caenorhabditis elegans larval arrest wormbase
YGR246C Saccharomyces cerevisiae inviable yeastgenome
TGME49_207900 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_207900 (Toxoplasma gondii), transcription initiation factor TFIIIB
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