Detailed view for PF3D7_1434500

Basic information

TDR Targets ID: 2657
Plasmodium falciparum, dynein-related AAA-type ATPase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.9414 | Length (AA): 8105 | MW (Da): 971013 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 9

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07728   AAA domain (dynein-related subfamily)

Gene Ontology

Mouse over links to read term descriptions.
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

There are 17 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1576 1989 2ce7 (A) 154 603 16.00 0.0000028 0.98 0.08 0.39
1592 1849 2bjv (A) 11 257 16.00 0 0.57 -0.08 -0.28
2451 2869 1fnn (A) 1 388 10.00 0 0.96 -0.16 0.16
1576 3672 3vkh (B) 1937 4029 13.00 0 1 -0.113171 1.64
1610 1791 5c3c (A) 33 209 26.00 0.63 0.99 0.157855 0.31
1612 3875 4akg (A) 1791 4053 12.00 0 1 -0.0695663 1.41
3910 4958 4wd9 (A) 36 1008 15.00 0 1 -0.140474 1.42
4079 4232 4ux7 (A) 80 235 29.00 0.56 0.76 0.139401 0.83
4883 5743 2x19 (B) 42 946 9.00 0 0.81 -0.0256693 0.33
5361 5678 4hte (A) 256 578 24.00 0.18 0.8 0.076635 0.6
5371 5752 4dkj (A) 59 370 30.00 0.013 0.07 -0.157469 2.09
5475 5803 4wat (A) 152 513 23.00 0.25 0.97 0.197992 0.37
6140 7038 1wa5 (C) 71 955 13.00 0 1 -0.00698081 0.39
6458 7741 3ffz (A) 3 1239 16.00 0 0.95 -0.141479 1.07
6949 7279 4k1p (C) 30 349 11.00 0 0.03 -0.017061 -1.22
7037 7327 3etv (A) 9 347 13.00 0 0.01 -0.0609962 -0.77
7218 7478 3fby (A) 316 530 28.00 0.056 0.02 -0.00339766 0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, Ring, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 24 hs, sporozoite, early ring, early trophozoite, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 32 hs, Oocyst, Sporozoite, Female Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs. Otto TD
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_127263)

Species Accession Gene Product
Arabidopsis thaliana AT1G67120   protein MIDASIN1
Babesia bovis BBOV_III002250   ATPase associated with various cellular activities (AAA) family protein
Brugia malayi Bm1_25520   hypothetical protein
Candida albicans CaO19.12167   similar to S. cerevisiae YLR106C
Candida albicans CaO19.12161   similar to N terminus of midasin-like orf
Candida albicans CaO19.4697   similar to midasin, highly conserved, putative nuclear chaperone
Caenorhabditis elegans CELE_F55F10.1   Protein F55F10.1, isoform B
Cryptosporidium hominis Chro.50062   hypothetical protein
Cryptosporidium parvum cgd5_3110   MDN1, midasin
Dictyostelium discoideum DDB_G0295765   type A von Willebrand factor domain-containing protein
Drosophila melanogaster Dmel_CG13185   CG13185 gene product from transcript CG13185-RD
Echinococcus granulosus EgrG_000061900   hypothetical protein
Echinococcus granulosus EgrG_000887300   midasin
Entamoeba histolytica EHI_153780   hypothetical protein
Echinococcus multilocularis EmuJ_000887300   midasin
Giardia lamblia GL50803_39312   Midasin
Homo sapiens ENSG00000112159   MDN1, midasin homolog (yeast)
Leishmania braziliensis LbrM.20.4470   hypothetical protein, conserved
Leishmania braziliensis LbrM.20.4460   hypothetical protein, conserved
Leishmania donovani LdBPK_200280.1   Midasin, putative
Leishmania infantum LinJ.20.0280   hypothetical protein, conserved
Leishmania major LmjF.20.0260   hypothetical protein, conserved
Leishmania mexicana LmxM.20.0260   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_14100   hypothetical protein
Loa Loa (eye worm) LOAG_15966   hypothetical protein
Loa Loa (eye worm) LOAG_06453   hypothetical protein
Loa Loa (eye worm) LOAG_15940   hypothetical protein
Loa Loa (eye worm) LOAG_06452   hypothetical protein
Loa Loa (eye worm) LOAG_16062   hypothetical protein
Loa Loa (eye worm) LOAG_15992   hypothetical protein
Loa Loa (eye worm) LOAG_10210   hypothetical protein
Loa Loa (eye worm) LOAG_02751   serine/threonine protein kinase
Loa Loa (eye worm) LOAG_15860   hypothetical protein
Mus musculus ENSMUSG00000058006   midasin homolog (yeast)
Neospora caninum NCLIV_017650   hypothetical protein
Oryza sativa 4348797   Os10g0456900
Onchocerca volvulus OVOC12265   Midasin homolog
Plasmodium berghei PBANKA_1010300   dynein-related AAA-type ATPase, putative
Plasmodium falciparum PF3D7_1434500   dynein-related AAA-type ATPase, putative
Plasmodium knowlesi PKNH_0421100   dynein-related AAA-type ATPase, putative
Plasmodium vivax PVX_084810   dynein-related AAA-type ATPase, putative
Plasmodium yoelii PY03141   dentin sialophosphoprotein precursor
Plasmodium yoelii PY00634   hypothetical protein
Plasmodium yoelii PY03140   Drosophila melanogaster SD07158p-related
Saccharomyces cerevisiae YLR106C   AAA family ATPase midasin
Schistosoma japonicum Sjp_0305860   Midasin, putative
Schistosoma japonicum Sjp_0008510   Midasin, putative
Schistosoma japonicum Sjp_0008500   ko:K01509 adenosinetriphosphatase [EC3.6.1.3], putative
Schistosoma japonicum Sjp_0113040   Midasin, putative
Schistosoma japonicum Sjp_0135260   expressed protein
Schistosoma mansoni Smp_143650   midasin (midas-containing protein)
Schistosoma mansoni Smp_143660   mdn1 midasin-related
Schmidtea mediterranea mk4.037686.01  
Schmidtea mediterranea mk4.037686.00   Midasin
Schmidtea mediterranea mk4.003346.00   Midasin
Schmidtea mediterranea mk4.003346.02  
Schmidtea mediterranea mk4.004262.01  
Trypanosoma brucei gambiense Tbg972.1.290   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0419   AAA domain (dynein-related subfamily), putative
Trypanosoma brucei Tb927.1.880   Midasin, putative
Trypanosoma brucei Tb11.v5.0418   AAA domain (dynein-related subfamily), putative
Trypanosoma congolense TcIL3000_1_370   Midasin, putative
Toxoplasma gondii TGME49_242625   ATPase family associated with various cellular activities (AAA) subfamily protein
Theileria parva TP03_0590   hypothetical protein
Theileria parva TP03_0591   hypothetical protein
Trichomonas vaginalis TVAG_243360   midasin, putative

Essentiality

PF3D7_1434500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.880 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.1.880 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.1.880 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.880 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F55F10.1 Caenorhabditis elegans larval arrest wormbase
CELE_F55F10.1 Caenorhabditis elegans slow growth wormbase
YLR106C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1010300 Plasmodium berghei Essential plasmo
TGME49_242625 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1434500 (Plasmodium falciparum), dynein-related AAA-type ATPase, putative
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