Detailed view for LmjF.32.0540

Basic information

TDR Targets ID: 26717
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.227 | Length (AA): 511 | MW (Da): 54952 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 10

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01925   Sulfite exporter TauE/SafE

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
379 507 4fc4 (A) 29 160 30.00 0.44 0.1 0.245446 1.7

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_130548)

Species Accession Gene Product
Arabidopsis thaliana AT2G25737   Sulfite exporter TauE/SafE family protein
Arabidopsis thaliana AT1G61740   Sulfite exporter TauE/SafE family protein
Arabidopsis thaliana AT4G21250   Sulfite exporter TauE/SafE family protein
Dictyostelium discoideum DDB_G0269644   hypothetical protein
Entamoeba histolytica EHI_078310   hypothetical protein, conserved
Giardia lamblia GL50803_4181   Hypothetical protein
Leishmania braziliensis LbrM.32.0550   hypothetical protein, conserved
Leishmania donovani LdBPK_320570.1   sulfite exporter TauE/SafE, putative
Leishmania infantum LinJ.32.0570   hypothetical protein, conserved
Leishmania major LmjF.32.0540   hypothetical protein, conserved
Leishmania mexicana LmxM.31.0540   hypothetical protein, conserved
Neospora caninum NCLIV_020390   hypothetical protein
Neospora caninum NCLIV_063190   hypothetical protein, conserved
Oryza sativa 4346797   Os09g0344800
Oryza sativa 4327050   Os01g0786800
Oryza sativa 4345471   Os08g0389700
Oryza sativa 4345461   Os08g0387200
Oryza sativa 4327049   Os01g0786700
Trypanosoma cruzi TcCLB.509161.140   hypothetical protein, conserved
Toxoplasma gondii TGME49_246178   hypothetical protein
Toxoplasma gondii TGME49_205300   hypothetical protein
Trichomonas vaginalis TVAG_288660   conserved hypothetical protein

Essentiality

LmjF.32.0540 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_246178 Toxoplasma gondii Essentiality uncertain sidik
TGME49_205300 Toxoplasma gondii Essentiality uncertain sidik
TGME49_246178 Toxoplasma gondii Probably non-essential sidik
TGME49_205300 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.32.0540 (Leishmania major), hypothetical protein, conserved
Title for this comment
Comment