Detailed view for LmjF.35.0070

Basic information

TDR Targets ID: 27165
Leishmania major, prohibitin, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.4711 | Length (AA): 292 | MW (Da): 32327 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01145   SPFH domain / Band 7 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
77 201 1win (A) 8 138 10.00 0.0000078 0.24 0.61 -1.12
79 190 2rpb (A) 66 174 23.00 0 1 0.724462 -0.73
80 190 4fvg (A) 95 202 20.00 0 0.82 0.737037 -1.38
157 223 4tql (A) 153 219 37.00 0.78 0.09 0.530152 0.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127772)

Species Accession Gene Product
Arabidopsis thaliana AT4G28510   prohibitin 1
Arabidopsis thaliana AT1G03860   prohibitin 2
Arabidopsis thaliana AT5G44140   prohibitin 7
Arabidopsis thaliana AT2G20530   prohibitin 6
Babesia bovis BBOV_I004460   prohibitin, putative
Brugia malayi Bm1_18290   Hypothetical 31.8 kDa protein in chromosome II, putative
Candida albicans CaO19.5973   similar to S. cerevisiae PHB2 (YGR231C) possible mitochondrial chaperone that affects mitochondrial segregation.
Candida albicans CaO19.13394   similar to S. cerevisiae PHB2 (YGR231C) possible mitochondrial chaperone that affects mitochondrial segregation.
Caenorhabditis elegans CELE_T24H7.1   Protein PHB-2
Cryptosporidium hominis Chro.50147   SPFH domain / Band 7 family
Cryptosporidium parvum cgd5_2320   putative prohibitin with PHB domain
Dictyostelium discoideum DDB_G0284117   hypothetical protein
Drosophila melanogaster Dmel_CG15081   Prohibitin 2
Echinococcus granulosus EgrG_000435200   prohibitin
Echinococcus multilocularis EmuJ_000435200   prohibitin
Homo sapiens ENSG00000215021   prohibitin 2
Leishmania braziliensis LbrM.34.0110   prohibitin, putative
Leishmania donovani LdBPK_350070.1   prohibitin, putative
Leishmania infantum LinJ.35.0070   prohibitin, putative
Leishmania major LmjF.35.0070   prohibitin, putative
Leishmania mexicana LmxM.34.0070   prohibitin, putative
Loa Loa (eye worm) LOAG_08798   hypothetical protein
Mus musculus ENSMUSG00000004264   prohibitin 2
Neospora caninum NCLIV_032910   hypothetical protein
Oryza sativa 4334737   Os03g0841700
Oryza sativa 4342874   Os07g0262200
Plasmodium berghei PBANKA_1213100   prohibitin, putative
Plasmodium falciparum PF3D7_1014700   prohibitin, putative
Plasmodium knowlesi PKNH_0814800   prohibitin, putative
Plasmodium vivax PVX_094950   prohibitin, putative
Plasmodium yoelii PY07442   SPFH domain / Band 7 family, putative
Saccharomyces cerevisiae YGR231C   prohibitin subunit PHB2
Schistosoma japonicum Sjp_0095920   Prohibitin-2, putative
Schistosoma mansoni Smp_075210.1   prohibitin
Schistosoma mansoni Smp_075210.2   prohibitin
Schmidtea mediterranea mk4.001779.01   Prohibitin-1
Trypanosoma brucei gambiense Tbg972.10.5350   prohibitin, putative
Trypanosoma brucei Tb10.v4.0045   prohibitin, putative
Trypanosoma brucei Tb927.10.4310   prohibitin 2, putative
Trypanosoma congolense TcIL3000_10_3600   prohibitin, putative
Trypanosoma cruzi TcCLB.511469.70   prohibitin, putative
Trypanosoma cruzi TcCLB.506295.130   prohibitin, putative
Toxoplasma gondii TGME49_233100   SPFH domain / Band 7 family protein
Theileria parva TP01_0188   prohibitin, putative

Essentiality

LmjF.35.0070 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_T24H7.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_T24H7.1 Caenorhabditis elegans slow growth wormbase
PBANKA_1213100 Plasmodium berghei Essential plasmo
TGME49_233100 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.35.0070 (Leishmania major), prohibitin, putative
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