Detailed view for PF3D7_1443000

Basic information

TDR Targets ID: 2739
Plasmodium falciparum, serine/threonine protein kinase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.7124 | Length (AA): 1268 | MW (Da): 150096 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 21 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
768 1014 1x8b (A) 295 561 14.00 0 0.75 0.33 -0.25
769 1266 2eu9 (A) 153 479 27.00 0 1 0.09 0.77
763 1193 3llt (A) 543 865 25.00 0 1 0.166305 1.5
763 1266 2eu9 (A) 147 479 23.00 0 1 0.00887634 1.45
764 1038 4usf (B) 26 299 16.00 0 0.83 0.379277 -0.03
765 928 1x8b (A) 292 474 21.00 0.0000041 0.41 0.198738 0.68
769 928 4jr7 (A) 37 225 23.00 0.00012 1 0.140583 0.12
978 1266 2exe (A) 245 479 23.00 0.0000000022 0.46 0.089618 0.64
1041 1100 4yno (A) 163 223 53.00 0.017 0.1 0.191419 1.76
1060 1113 2b9h (A) 182 239 43.00 0.21 0.39 0.338687 0.53
1060 1259 3oz6 (A) 190 332 38.00 0.023 0.8 0.00882871 0.99
1062 1198 4bgq (A) 162 301 26.00 0.00017 0.43 0.320744 -0.5
39 385 2fds (A) 28 324 29.00 0.99 0.07 0.22147 0.98
749 991 2pml (X) 6 273 26.00 0.05 0.99 0.237838 0.92
764 934 1q8y (A) 149 567 23.00 0.000000000033 0.8 0.329423 -0.14
770 1266 1wak (A) 79 654 28.00 0.0000000013 1 0.00140989 1.17
770 965 3ddq (C) 2 198 19.00 0 0.27 0.228046 0.44
784 1266 3llt (A) 570 875 36.00 0.00000000019 1 0.130221 0.79
1047 1106 4yno (A) 163 223 53.00 0.016 0.16 0.169196 2.04
1066 1119 2b9h (A) 182 239 43.00 0.21 0.12 0.325486 0.39
1068 1204 4bgq (A) 162 301 26.00 0.00027 0.44 0.320835 -0.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, gametocyte, late trophozoite, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Ring. Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_165485)

Species Accession Gene Product
Plasmodium berghei PBANKA_1306900   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_1443000   serine/threonine protein kinase
Plasmodium knowlesi PKNH_1239300   serine/threonine protein kinase, putative
Plasmodium vivax PVX_118340   serine/threonine protein kinase, putative
Plasmodium yoelii PY00095   putative protein kinase

Essentiality

PF3D7_1443000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1306900 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0032 0.5 0.5
0.0093 0.8828 0
0.0056 1 0.5
0.0081 1 0.5
0.0088 0.4477 0.5
0.0064 0.3377 0
0.0059 1 1
0.0022 0.5 0.5
0.0063 0.7244 0.7244
0.0007 0.5 0.5
0.0033 1 1
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0037 1 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0007 0.5 0.5
0.0004 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0033 0.5 0.5
0.0063 1 1
0.0059 1 1
0.0062 0.6935 0
0.0008 0.5 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0036 0.5 0.5
0.0061 0.6883 1
0.0039 0.5 0.5
0.0026 0.5 0.5
0.0066 0.3101 0
0.0027 1 0.5
0.0067 0.5 0.5
0.0016 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1443000 (Plasmodium falciparum), serine/threonine protein kinase
Title for this comment
Comment