Detailed view for PF3D7_1444500
Basic information
Plasmodium falciparum, eukaryotic initiation factor 2alpha kinase 1
Source Database / ID: PlasmoDB | GeneDB | MPMP
pI: 7.9593 |
Length (AA): 1558 |
MW (Da): 184251 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004713 protein tyrosine kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004672 protein kinase activity
GO:0005524 ATP binding
GO:0006468 protein amino acid phosphorylation
GO:0006412 translation
Metabolic Pathways
Structural information
Modbase 3D models:
There are 14 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 515 | 841 | 1koa () | 5917 | 6256 | 18.00 | 0 | 1 | 0.42 | -0.27 |
| 611 | 785 | 1t46 (A) | 668 | 922 | 22.00 | 0.000000000036 | 1 | 0.42 | -1.23 |
| 814 | 1527 | 3bta (A) | 271 | 1054 | 17.00 | 0.000000027 | 0.72 | 0.41 | 0.88 |
| 2 | 778 | 5dyk (A) | 51 | 782 | 18.00 | 0.0034 | 0.8 | 0.348216 | 1.3 |
| 421 | 777 | 2a19 (B) | 259 | 526 | 42.00 | 0 | 1 | 0.0779399 | 1.15 |
| 572 | 1022 | 3q5i (A) | 75 | 503 | 31.00 | 0.000000000033 | 1 | 0.367274 | 0.56 |
| 593 | 792 | 1phk (A) | 83 | 290 | 23.00 | 0 | 1 | 0.36977 | -0.45 |
| 628 | 783 | 4bf2 (A) | 771 | 932 | 30.00 | 0.011 | 1 | 0.325628 | -0.76 |
| 2 | 793 | 5dyk (A) | 51 | 803 | 17.00 | 0.0000097 | 0.86 | 0.427098 | 1.27 |
| 421 | 772 | 2a19 (B) | 259 | 526 | 42.00 | 0 | 1 | 0.0998434 | 1.12 |
| 546 | 790 | 2vd5 (A) | 82 | 345 | 21.00 | 0 | 1 | 0.371867 | -0.3 |
| 567 | 784 | 2y7j (A) | 66 | 291 | 21.00 | 0.000046 | 1 | 0.335536 | -0.88 |
| 567 | 1017 | 3q5i (A) | 75 | 503 | 31.00 | 0.000000000028 | 1 | 0.369756 | 0.56 |
| 807 | 1549 | 3s5h (A) | 131 | 958 | 16.00 | 0.0023 | 0.71 | 0.294464 | 1.33 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Upper 60-80% percentile | intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, merozoite, sporozoite, early ring, Ring, Sporozoite, Male Gametocyte. | Otto TD PlasmoDB Zanghi G Lasonder E |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Mid 40-60% percentile | late ring, Oocyst, Female Gametocyte. | PlasmoDB Zanghi G Lasonder E |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Lower 20-40% percentile | intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont. | Otto TD PlasmoDB |
-
Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq. -
Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. -
PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB -
Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
Orthologs
Ortholog group members (OG5_150177)
| Species | Accession | Gene Product |
|---|---|---|
| Cryptosporidium hominis | Chro.30368 | hypothetical protein |
| Cryptosporidium parvum | cgd3_3230 | Ser/thr protein kinase |
| Plasmodium berghei | PBANKA_1308400 | GCN2 alpha-related protein kinase, putative |
| Plasmodium falciparum | PF3D7_1444500 | eukaryotic initiation factor 2alpha kinase 1 |
| Plasmodium knowlesi | PKNH_1237800 | serine/threonine protein kinase, putative |
| Plasmodium vivax | PVX_118270 | serine/threonine protein kinase, putative |
| Plasmodium yoelii | PY03319 | Mus musculus GCN2alpha |
| Theileria parva | TP02_0664 | hypothetical protein |
Essentiality
Phenotypes and Validation (curated)
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Druggability index (range: 0 to 1): 0.5
Known modulators for this target
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
Obtained from network model
Ranking Plot
Putative Drugs List
Assayability
Assay information
Reagent availability
Bibliographic References