Detailed view for LmjF.07.0010

Basic information

TDR Targets ID: 27951
Leishmania major, ubiquitin activating E1 enzyme, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2804 | Length (AA): 725 | MW (Da): 79061 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family
PF16420   Ubiquitin-like modifier-activating enzyme ATG7 N-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0008641   small protein activating enzyme activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006914   autophagy  

Metabolic Pathways

Regulation of autophagy (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
360 670 1jw9 (B) 3 248 27.00 0 1 0.38 -0.42
391 555 1jw9 (B) 33 189 35.00 0.00000001 1 0.65 -1.33
6 705 4gsl (A) 3 608 33.00 0 1 1.14112 0.53
365 533 1zfn (B) 2 154 33.00 0.00012 1 0.480703 0.16
385 617 1jw9 (B) 27 217 30.00 0 1 0.455779 -0.19
396 508 3slk (A) 517 643 36.00 0.59 0.6 0.362462 0.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128264)

Species Accession Gene Product
Arabidopsis thaliana AT5G45900   ubiquitin-like modifier-activating enzyme atg7
Babesia bovis BBOV_IV008170   hypothetical protein
Brugia malayi Bm1_39695   E1-like protein-activating enzyme Gsa7p/Apg7p containing protein
Candida albicans CaO19.707   similar to S. cerevisiae ATG7 (YHR171W) protein-conjugating enzyme of the Apg12p-Apg5p conjugation pathway of autophagy
Candida albicans CaO19.8326   similar to S. cerevisiae ATG7 (YHR171W) protein-conjugating enzyme of the Apg12p-Apg5p conjugation pathway of autophagy
Caenorhabditis elegans CELE_M7.5   Protein ATG-7
Cryptosporidium hominis Chro.40017   hypothetical protein
Cryptosporidium parvum cgd4_40   APG7-like ubiquitin activating enzyme E1
Dictyostelium discoideum DDB_G0271096   E1-like enzyme family protein
Drosophila melanogaster Dmel_CG5489   Autophagy-specific gene 7
Echinococcus granulosus EgrG_001055800   ubiquitin modifier activating enzyme ATG7
Entamoeba histolytica EHI_064700   autophagy protein apg7, putative
Echinococcus multilocularis EmuJ_001055800   ubiquitin modifier activating enzyme ATG7
Homo sapiens ENSG00000197548   autophagy related 7
Leishmania braziliensis LbrM.07.0010   ubiquitin activating E1 enzyme, putative
Leishmania donovani LdBPK_070010.1   Ubiquitin-like modifier-activating enzyme ATG7, putative
Leishmania infantum LinJ.07.0010   ubiquitin activating E1 enzyme, putative
Leishmania major LmjF.07.0010   ubiquitin activating E1 enzyme, putative
Leishmania mexicana LmxM.07.0010   ubiquitin activating E1 enzyme, putative
Loa Loa (eye worm) LOAG_08126   hypothetical protein
Mus musculus ENSMUSG00000030314   autophagy related 7
Neospora caninum NCLIV_030510   thiF family domain-containing protein, putative
Oryza sativa 4326644   Os01g0614900
Plasmodium berghei PBANKA_0922200   autophagy-related protein 7, putative
Plasmodium falciparum PF3D7_1126100   autophagy-related protein 7, putative
Plasmodium knowlesi PKNH_0924000   autophagy-related protein 7, putative
Plasmodium vivax PVX_091922   autophagy-related protein 7, putative
Plasmodium yoelii PY06467   ubiquitin activating enzyme E1-like protein-related
Saccharomyces cerevisiae YHR171W   Atg7p
Schistosoma japonicum Sjp_0102790   ko:K08337 autophagy-related protein 7, putative
Schistosoma japonicum Sjp_0303750   ko:K08337 autophagy-related protein 7, putative
Schmidtea mediterranea mk4.005587.01  
Trypanosoma brucei gambiense Tbg972.10.13510   ubiquitin activating E1 enzyme, putative,autophagy protein, putative
Trypanosoma brucei Tb927.10.11180   Ubiquitin-like modifier-activating enzyme ATG7, putative
Trypanosoma congolense TcIL3000_10_9420   Ubiquitin-like modifier-activating enzyme ATG7, putative
Trypanosoma cruzi TcCLB.509641.30   Ubiquitin-like modifier-activating enzyme ATG7, putative
Trypanosoma cruzi TcCLB.507711.150   Ubiquitin-like modifier-activating enzyme ATG7, putative
Toxoplasma gondii TGME49_229690   autophagy-related protein 7 atg7, putative
Theileria parva TP01_0958   hypothetical protein
Trichomonas vaginalis TVAG_092470   autophagy protein, putative

Essentiality

LmjF.07.0010 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.11180 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.11180 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.11180 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.11180 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_229690 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens autophagy related 7 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0048 0.4768 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.07.0010 (Leishmania major), ubiquitin activating E1 enzyme, putative
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