Detailed view for LmjF.35.4500

Basic information

TDR Targets ID: 28049
Leishmania major, anaphase promoting complex subunit protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6022 | Length (AA): 82 | MW (Da): 9358 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12861   Anaphase-promoting complex subunit 11 RING-H2 finger

Gene Ontology

Mouse over links to read term descriptions.
GO:0005680   anaphase-promoting complex  
GO:0008270   zinc ion binding  
GO:0005515   protein binding  
GO:0004842   ubiquitin-protein ligase activity  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
19 77 1iym (A) 130 176 34.00 0.00000000038 0.97 0.79 1.02
32 77 1f62 (A) 1 48 46.00 0.000012 0.97 1.05 0.08
2 82 3dpl (R) 21 103 31.00 0 0.56 1.2408 0.34
20 75 4d0e (A) 472 518 49.00 0.21 0.09 0.877227 0.58
21 82 4r2y (B) 21 82 46.00 0 1 1.2177 -0.41
31 79 2yur (A) 15 61 30.00 0 0.75 0.938161 -0.6
34 81 3vk6 (A) 4 48 31.00 0 0.99 0.979966 -1.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129026)

Species Accession Gene Product
Arabidopsis thaliana AT3G05870   anaphase-promoting complex subunit 11
Brugia malayi Bm1_37620   putative anaphase promoting complex subunit 11
Brugia malayi Bm1_49060   putative anaphase promoting complex subunit 11
Candida albicans CaO19.7644   similar to S. cerevisiae APC11 (YDL008W) anaphase promoting complex subunit
Caenorhabditis elegans CELE_F35G12.9   Protein APC-11
Cryptosporidium hominis Chro.10298   hypothetical protein
Cryptosporidium parvum cgd1_2640   hypothetical protein with RING-H2 like RING domain
Dictyostelium discoideum DDB_G0286909   anaphase promoting complex subunit 11
Drosophila melanogaster Dmel_CG34440   lemming A
Echinococcus granulosus EgrG_000069420   anaphase promoting complex subunit 11
Entamoeba histolytica EHI_135100   zinc finger domain containing protein
Echinococcus multilocularis EmuJ_000069420   anaphase promoting complex subunit 11
Giardia lamblia GL50803_8432   APC11, cyclin metabolism
Leishmania braziliensis LbrM.34.4480   anaphase promoting complex subunit protein, putative
Leishmania donovani LdBPK_354570.1   anaphase promoting complex subunit protein, putative
Leishmania infantum LinJ.35.4570   anaphase promoting complex subunit protein, putative
Leishmania major LmjF.35.4500   anaphase promoting complex subunit protein, putative
Leishmania mexicana LmxM.34.4500   anaphase promoting complex subunit protein, putative
Loa Loa (eye worm) LOAG_01568   hypothetical protein
Loa Loa (eye worm) LOAG_00857   hypothetical protein
Mus musculus ENSMUSG00000025135   anaphase promoting complex subunit 11
Neospora caninum NCLIV_038660   hypothetical protein, conserved
Oryza sativa 4332583   Os03g0302700
Oryza sativa 9272104   Os07g0411101
Onchocerca volvulus OVOC4367  
Onchocerca volvulus OVOC864  
Plasmodium berghei PBANKA_1123400   anaphase-promoting complex subunit 11, putative
Plasmodium falciparum PF3D7_0624500   anaphase-promoting complex subunit 11, putative
Plasmodium knowlesi PKNH_1125700   anaphase-promoting complex subunit 11, putative
Plasmodium vivax PVX_114337   anaphase promoting complex subunit, putative
Plasmodium yoelii PY04444   putative APC11 anaphase-promoting complex subunit
Saccharomyces cerevisiae YDL008W   anaphase promoting complex subunit 11
Schistosoma japonicum Sjp_0217080   ko:K03358 anaphase-promoting complex component APC11, putative
Schistosoma mansoni Smp_028430   Anaphase promoting complex subunit 11 homolog
Schmidtea mediterranea mk4.000589.04   AT07979p1
Schmidtea mediterranea mk4.001942.04  
Trypanosoma brucei gambiense Tbg972.9.5890   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.10170   anaphase-promoting complex subunit 11
Trypanosoma cruzi TcCLB.507007.45   anaphase promoting complex subunit protein, putative
Trypanosoma cruzi TcCLB.509551.95   anaphase promoting complex subunit protein, putative
Toxoplasma gondii TGME49_267520   anaphase promoting complex subunit 11, putative
Trichomonas vaginalis TVAG_169830   RING finger, putative
Trichomonas vaginalis TVAG_389590   RING finger, putative

Essentiality

LmjF.35.4500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.1655 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.1655 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.1655 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.1655 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F35G12.9 Caenorhabditis elegans embryonic lethal wormbase
CELE_F35G12.9 Caenorhabditis elegans sterile wormbase
YDL008W Saccharomyces cerevisiae inviable yeastgenome
TGME49_267520 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.35.4500 (Leishmania major), anaphase promoting complex subunit protein, putative
Title for this comment
Comment