Detailed view for PF3D7_1451100

Basic information

TDR Targets ID: 2816
Plasmodium falciparum, elongation factor 2
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.7655 | Length (AA): 832 | MW (Da): 93523 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00009   Elongation factor Tu GTP binding domain
PF00679   Elongation factor G C-terminus
PF03144   Elongation factor Tu domain 2
PF03764   Elongation factor G, domain IV
PF14492   Elongation Factor G, domain II

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0003746   translation elongation factor activity  
GO:0006414   translational elongation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 832 1n0u (A) 4 842 59.00 0 1 1.56 -1.07
4 832 1n0u (A) 4 842 61.00 0 1 1.72849 -0.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Oocyst, Ring, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_126923)

Species Accession Gene Product
Arabidopsis thaliana AT3G12915   ribosomal protein S5/Elongation factor G/III/V family protein
Arabidopsis thaliana AT1G56070   elongation factor EF-2-like protein LOS1
Babesia bovis BBOV_I003090   elongation factor 2, EF-2
Brugia malayi Bm1_28480   translation elongation factor aEF-2
Candida albicans CaO19.13210   Translation elongation factor EF-2
Candida albicans CaO19.5788   Translation elongation factor EF-2
Caenorhabditis elegans CELE_F25H5.4   Protein EEF-2, isoform B
Cryptosporidium hominis Chro.80341   elongation factor 2 (EF-2)
Cryptosporidium parvum cgd8_2930   Eft2p GTpase; translation elongation factor 2 (EF-2)
Dictyostelium discoideum DDB_G0287685   elongation factor 2
Dictyostelium discoideum DDB_G0288373   elongation factor 2
Drosophila melanogaster Dmel_CG2238   Elongation factor 2
Echinococcus granulosus EgrG_000865300   elongation factor 2
Entamoeba histolytica EHI_166810   elongation factor 2
Entamoeba histolytica EHI_189490   elongation factor 2
Entamoeba histolytica EHI_164510   elongation factor 2
Echinococcus multilocularis EmuJ_000865300   elongation factor 2
Giardia lamblia GL50803_17570   Elongation factor 2
Homo sapiens ENSG00000108883   elongation factor Tu GTP binding domain containing 2
Homo sapiens ENSG00000167658   eukaryotic translation elongation factor 2
Leishmania braziliensis LbrM.35.0270   elongation factor 2
Leishmania donovani LdBPK_360210.1   elongation factor 2
Leishmania donovani LdBPK_360190.1   elongation factor 2
Leishmania infantum LinJ.36.0200   elongation factor 2
Leishmania infantum LinJ.36.0210   elongation factor 2
Leishmania infantum LinJ.36.0190   elongation factor 2
Leishmania major LmjF.36.0180   elongation factor 2
Leishmania major LmjF.36.0190   elongation factor 2
Leishmania mexicana LmxM.36.0180   elongation factor 2
Loa Loa (eye worm) LOAG_05446   translation elongation factor aEF-2
Mus musculus ENSMUSG00000020929   elongation factor Tu GTP binding domain containing 2
Mus musculus ENSMUSG00000034994   eukaryotic translation elongation factor 2
Neospora caninum NCLIV_020220   elongation factor 2, putative
Oryza sativa 4324669   Os01g0723000
Oryza sativa 4329493   Os02g0519900
Oryza sativa 4326544   Os01g0742200
Oryza sativa 4334959   Os04g0118400
Plasmodium berghei PBANKA_1314800   elongation factor 2, putative
Plasmodium falciparum PF3D7_1451100   elongation factor 2
Plasmodium knowlesi PKNH_1231000   elongation factor 2, putative
Plasmodium vivax PVX_117925   elongation factor 2, putative
Plasmodium yoelii PY05356   elongation factor 2
Saccharomyces cerevisiae YOR133W   elongation factor 2
Saccharomyces cerevisiae YDR385W   elongation factor 2
Schistosoma japonicum Sjp_0012720   ko:K03234 elongation factor EF-2, putative
Schistosoma mansoni Smp_143150   eukaryotic translation elongation factor
Schmidtea mediterranea mk4.000542.01  
Schmidtea mediterranea mk4.003189.00  
Schmidtea mediterranea mk4.002871.03  
Schmidtea mediterranea mk4.008935.01   Putative eukaryotic translation elongation factor
Schmidtea mediterranea mk4.004544.07   Putative eukaryotic translation elongation factor
Schmidtea mediterranea mk4.017031.02   Putative eukaryotic translation elongation factor
Trypanosoma brucei gambiense Tbg972.10.5590   elongation factor 2, putative
Trypanosoma brucei Tb927.10.4560   elongation factor 2
Trypanosoma brucei Tb927.10.4570   elongation factor 2
Trypanosoma congolense TcIL3000_10_3760   elongation factor 2, putative
Trypanosoma cruzi TcCLB.510963.90   elongation factor 2, putative
Trypanosoma cruzi TcCLB.508625.10   elongation factor 2, putative
Trypanosoma cruzi TcCLB.508899.151   elongation factor 2, putative
Trypanosoma cruzi TcCLB.508169.20   elongation factor 2, putative
Toxoplasma gondii TGME49_205470   translation elongation factor 2 family protein, putative
Theileria parva TP01_0529   elongation factor 2, putative
Trichomonas vaginalis TVAG_016880   translation factor, putative
Trichomonas vaginalis TVAG_276410   translation elongation factor, putative

Essentiality

PF3D7_1451100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.4560 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.4560 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.4560 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.4560 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F25H5.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F25H5.4 Caenorhabditis elegans larval arrest wormbase
CELE_F25H5.4 Caenorhabditis elegans slow growth wormbase
CELE_F25H5.4 Caenorhabditis elegans sterile wormbase
PBANKA_1314800 Plasmodium berghei Essential plasmo
TGME49_205470 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1451100 (Plasmodium falciparum), elongation factor 2
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