Detailed view for PF3D7_1451500

Basic information

TDR Targets ID: 2820
Plasmodium falciparum, pre-mRNA-splicing factor CWF18, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.7397 | Length (AA): 147 | MW (Da): 17257 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08315   cwf18 pre-mRNA splicing factor

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
18 142 4uos (A) 2 123 20.00 0.07 0.15 1.11554 -1.33
20 123 2fxm (B) 851 955 16.00 0.69 0 0.889683 -0.55

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 32 hs, Oocyst, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Sporozoite, Male Gametocyte. Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Female Gametocyte. Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_129682)

Species Accession Gene Product
Arabidopsis thaliana AT3G05070   hypothetical protein
Babesia bovis BBOV_I002710   conserved hypothetical protein
Brugia malayi Bm1_54230   zgc:101038 protein
Caenorhabditis elegans CELE_Y69A2AR.21   Protein Y69A2AR.21
Dictyostelium discoideum DDB_G0292648   mRNA splicing factor, Cwf18 family protein
Drosophila melanogaster Dmel_CG15525   CG15525 gene product from transcript CG15525-RA
Echinococcus granulosus EgrG_000817900   mRNA splicing factor Cwf18
Echinococcus multilocularis EmuJ_000817900   mRNA splicing factor, Cwf18
Homo sapiens ENSG00000160799   coiled-coil domain containing 12
Loa Loa (eye worm) LOAG_05143   hypothetical protein
Mus musculus ENSMUSG00000019659   coiled-coil domain containing 12
Neospora caninum NCLIV_038350   hypothetical protein, conserved
Oryza sativa 4342149   Os06g0730800
Onchocerca volvulus OVOC8929  
Plasmodium berghei PBANKA_1315200   pre-mRNA-splicing factor CWF18, putative
Plasmodium falciparum PF3D7_1451500   pre-mRNA-splicing factor CWF18, putative
Plasmodium knowlesi PKNH_1230600   pre-mRNA-splicing factor CWF18, putative
Plasmodium vivax PVX_117905   pre-mRNA-splicing factor CWF18, putative
Plasmodium yoelii PY01625   hypothetical protein
Schistosoma japonicum Sjp_0200080   IPR013169,mRNA splicing factor, Cwf18,domain-containing
Schistosoma mansoni Smp_080110   pre-mRNA splicing factor
Toxoplasma gondii TGME49_279430   cwf18 pre-mRNA splicing factor protein
Theileria parva TP01_0018   hypothetical protein

Essentiality

PF3D7_1451500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_Y69A2AR.21 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1315200 Plasmodium berghei Essential plasmo
TGME49_279430 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Pfal003403AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Pfal003403; Recombinant protein: full-length; Source: P falciparum; hypothetical protein ;

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_1451500 (Plasmodium falciparum), pre-mRNA-splicing factor CWF18, putative
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