Detailed view for LmjF.35.1260

Basic information

TDR Targets ID: 28335
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.9865 | Length (AA): 361 | MW (Da): 39866 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)

Gene Ontology

Mouse over links to read term descriptions.
GO:0017111   nucleoside-triphosphatase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 356 1e32 (A) 99 458 26.00 0 1 1.16 -0.19
2 81 1tmy () 38 117 5.00 0.000000061 0.01 0.41 -1.52
85 356 1e32 (A) 189 458 33.00 0 1 1.22 -0.78
77 360 4l15 (A) 293 568 32.00 0 1 1.166 -0.31
77 360 4ko8 (A) 181 462 27.00 0 1 1.148 -0.36
129 159 1sxj (D) 51 82 58.00 0.62 0.85 0.704773 0.09
132 166 1x6v (A) 47 83 40.00 0 0.51 0.450253 0.17
269 361 4a3v (B) 380 454 21.00 0 0.48 0.312918 -0.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128977)

Species Accession Gene Product
Arabidopsis thaliana AT4G27680   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Arabidopsis thaliana AT5G53540   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Brugia malayi Bm1_51360   AAA ATPase
Candida albicans CaO19.11840   intramitochondrial sorting protein, ATPase AAA family
Candida albicans CaO19.4362   intramitochondrial sorting protein, ATPase AAA family
Caenorhabditis elegans CELE_K04D7.2   Protein MSPN-1, isoform A
Dictyostelium discoideum DDB_G0276169   AAA ATPase domain-containing protein
Drosophila melanogaster Dmel_CG5395   no mitochondrial derivative
Drosophila melanogaster Dmel_CG4701   CG4701 gene product from transcript CG4701-RA
Echinococcus granulosus EgrG_000076600   ATPase family AAA domain containing protein 1 B
Echinococcus multilocularis EmuJ_000076600   ATPase family AAA domain containing protein 1 B
Homo sapiens ENSG00000138138   ATPase family, AAA domain containing 1
Leishmania braziliensis LbrM.34.1180   hypothetical protein, conserved
Leishmania donovani LdBPK_351270.1   ATPase family associated with various cellular activities (AAA), putative
Leishmania infantum LinJ.35.1270   hypothetical protein, conserved
Leishmania major LmjF.35.1260   hypothetical protein, conserved
Leishmania mexicana LmxM.34.1260   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_04750   AAA ATPase
Mus musculus ENSMUSG00000013662   ATPase family, AAA domain containing 1
Oryza sativa 4342062   Os06g0714500
Saccharomyces cerevisiae YGR028W   Msp1p
Schmidtea mediterranea mk4.000589.00   Mitochondrial sorting homolog
Trypanosoma brucei gambiense Tbg972.5.1320   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.960   atpase family aaa domain-containing protein 1
Trypanosoma congolense TcIL3000_0_47230   ATPase family associated with various cellular activities (AAA), putative
Trypanosoma cruzi TcCLB.508303.50   ATPase family associated with various cellular activities (AAA), putative
Trypanosoma cruzi TcCLB.511179.110   ATPase family associated with various cellular activities (AAA), putative

Essentiality

LmjF.35.1260 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.960 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.960 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.960 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.960 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_K04D7.2 Caenorhabditis elegans larval arrest wormbase
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.35.1260 (Leishmania major), hypothetical protein, conserved
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