Detailed view for LmjF.36.0080

Basic information

TDR Targets ID: 28481
Leishmania major, leucine carboxyl methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.7674 | Length (AA): 296 | MW (Da): 33451 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04072   Leucine carboxyl methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0032259   methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 285 1rjd (A) 4 326 27.00 0 1 1.17 -1.24
2 292 3iei (A) 25 327 29.00 0 1 1.37741 -0.91
3 285 1rjd (A) 5 326 27.00 0 1 1.18338 -0.54

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126898)

Species Accession Gene Product
Arabidopsis thaliana AT1G02100   Leucine carboxyl methyltransferase
Babesia bovis BBOV_III003160   hypothetical protein
Brugia malayi Bm1_32700   Leucine carboxyl methyltransferase family protein
Candida albicans CaO19.3303   carboxymethyltransferase
Candida albicans CaO19.13734   carboxy methyl transferase for protein phosphatase 2A
Candida albicans CaO19.6377   carboxy methyl transferase for protein phosphatase 2A
Candida albicans CaO19.10813   carboxymethyltransferase
Caenorhabditis elegans CELE_B0285.4   Protein B0285.4
Dictyostelium discoideum DDB_G0288271   leucine carboxyl methyltransferase
Drosophila melanogaster Dmel_CG3793   CG3793 gene product from transcript CG3793-RB
Echinococcus granulosus EgrG_000120900   leucine carboxyl methyltransferase 1
Echinococcus multilocularis EmuJ_000120900   leucine carboxyl methyltransferase 1
Giardia lamblia GL50803_10516   Leucine carboxyl methyltransferase
Homo sapiens ENSG00000162971   tRNA-yW synthesizing protein 5
Homo sapiens 9836   leucine carboxyl methyltransferase 2
Leishmania braziliensis LbrM.35.0150   leucine carboxyl methyltransferase, putative
Leishmania braziliensis LbrM.34.2850   hypothetical protein, conserved
Leishmania donovani LdBPK_352990.1   Leucine carboxyl methyltransferase/Cupin-like domain containing protein, putative
Leishmania donovani LdBPK_360090.1   leucine carboxyl methyltransferase, putative
Leishmania infantum LinJ.36.0090   leucine carboxyl methyltransferase, putative
Leishmania infantum LinJ.35.2990   hypothetical protein, conserved
Leishmania major LmjF.36.0080   leucine carboxyl methyltransferase, putative
Leishmania major LmjF.35.2940   hypothetical protein, conserved
Leishmania mexicana LmxM.34.2940   hypothetical protein, conserved
Leishmania mexicana LmxM.36.0080   leucine carboxyl methyltransferase, putative
Loa Loa (eye worm) LOAG_05792   hypothetical protein
Loa Loa (eye worm) LOAG_11076   leucine carboxyl methyltransferase
Mus musculus ENSMUSG00000074890   leucine carboxyl methyltransferase 2
Mus musculus ENSMUSG00000030763   leucine carboxyl methyltransferase 1
Mus musculus ENSMUSG00000048495   tRNA-yW synthesizing protein 5
Neospora caninum NCLIV_051310   Leucine carboxyl methyltransferase, related
Oryza sativa 4340066   Os06g0140100
Onchocerca volvulus OVOC3030   Putative leucine carboxyl methyltransferase 1
Plasmodium berghei PBANKA_1303600   leucine carboxyl methyltransferase, putative
Plasmodium falciparum PF3D7_1439700   leucine carboxyl methyltransferase, putative
Plasmodium knowlesi PKNH_1242600   leucine carboxyl methyltransferase, putative
Plasmodium vivax PVX_118505   leucine carboxyl methyltransferase, putative
Plasmodium yoelii PY00828   unknown protein-related
Saccharomyces cerevisiae YDR435C   Ppm1p
Saccharomyces cerevisiae YOL141W   Ppm2p
Schistosoma japonicum Sjp_0204500   ko:K00599 leucine carboxyl methyltransferase 1 [EC:2.1.1.-], putative
Schistosoma mansoni Smp_124860   hypothetical protein
Schmidtea mediterranea mk4.002399.01  
Schmidtea mediterranea mk4.027262.00   Probable leucine carboxyl methyltransferase 1
Schmidtea mediterranea mk4.009380.01   tRNA wybutosine-synthesizing protein 5
Schmidtea mediterranea mk4.001492.01  
Schmidtea mediterranea mk4.001492.02   tRNA wybutosine-synthesizing protein 4
Schmidtea mediterranea mk4.003235.01   Probable leucine carboxyl methyltransferase 1
Trypanosoma brucei gambiense Tbg972.10.5490   leucine carboxyl methyltransferase, putative
Trypanosoma brucei gambiense Tbg972.9.7830   hypothetical protein, conserved
Trypanosoma brucei Tb10.v4.0036   leucine carboxyl methyltransferase, putative
Trypanosoma brucei Tb927.10.4460   leucine carboxyl methyltransferase, putative
Trypanosoma brucei Tb927.9.12780   Leucine carboxyl methyltransferase/Cupin-like domain containing protein, putative
Trypanosoma congolense TcIL3000_10_3660   leucine carboxyl methyltransferase, putative
Trypanosoma congolense TcIL3000_9_5290   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510659.130   Leucine carboxyl methyltransferase/Cupin-like domain containing protein, putative
Trypanosoma cruzi TcCLB.509757.20   leucine carboxyl methyltransferase, putative
Trypanosoma cruzi TcCLB.508625.120   leucine carboxyl methyltransferase, putative
Trypanosoma cruzi TcCLB.508463.29   Leucine carboxyl methyltransferase/Cupin-like domain containing protein, putative
Toxoplasma gondii TGME49_237570   leucine carboxyl methyltransferase
Theileria parva TP03_0904   hypothetical protein
Theileria parva TP02_0471   hypothetical protein, conserved
Theileria parva TP04_0135   hypothetical protein, conserved
Theileria parva TP04_0130   hypothetical protein
Theileria parva TP04_0133   hypothetical protein
Theileria parva TP04_0131   hypothetical protein
Theileria parva TP04_0132   hypothetical protein
Theileria parva TP04_0134   hypothetical protein, conserved
Trichomonas vaginalis TVAG_044530   leucine carboxyl methyltransferase, putative
Trichomonas vaginalis TVAG_184240   leucine carboxyl methyltransferase, putative
Trichomonas vaginalis TVAG_003240   leucine carboxyl methyltransferase, putative
Trichomonas vaginalis TVAG_474040   leucine carboxyl methyltransferase, putative

Essentiality

LmjF.36.0080 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.3730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.3730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.3730 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.3730 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1303600 Plasmodium berghei Essential plasmo
TGME49_237570 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.36.0080 (Leishmania major), leucine carboxyl methyltransferase, putative
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