Detailed view for LmjF.35.4280

Basic information

TDR Targets ID: 28521
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9131 | Length (AA): 816 | MW (Da): 90224 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF16531   Centriolar protein SAS N-terminal

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
398 639 1u4q (A) 1679 1920 18.00 0.000041 0.57 0.48 -0.09
129 341 3q0x (A) 19 218 22.00 0 1 0.447129 -0.16
130 314 4ckn (B) 130 314 99.00 0 1 1.39152 -1.07
353 580 4tql (A) 14 236 14.00 0 0.55 0.444512 -1.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 4CKM:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4CKN:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4CKP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129816)

Species Accession Gene Product
Cryptosporidium hominis Chro.80062   hypothetical protein
Cryptosporidium parvum cgd8_490   uncharacterized large low complexity coiled coil protein
Drosophila melanogaster Dmel_CG15524   Spindle assembly abnormal 6 ortholog (C. elegans)
Echinococcus granulosus EgrG_000597000   spindle assembly abnormal protein 6
Echinococcus multilocularis EmuJ_000597000   spindle assembly abnormal protein 6
Homo sapiens ENSG00000156876   spindle assembly 6 homolog (C. elegans)
Leishmania braziliensis LbrM.34.4260   hypothetical protein, conserved
Leishmania donovani LdBPK_354350.1   Spindle assembly abnormal protein 6 homolog
Leishmania infantum LinJ.35.4350   hypothetical protein, conserved
Leishmania major LmjF.35.4280   hypothetical protein, conserved
Leishmania mexicana LmxM.34.4280   hypothetical protein, conserved
Mus musculus ENSMUSG00000027959   spindle assembly 6 homolog (C. elegans)
Neospora caninum NCLIV_044630   hypothetical protein, conserved
Plasmodium berghei PBANKA_0106200   spindle assembly abnormal protein 6
Plasmodium falciparum PF3D7_0607600   spindle assembly abnormal protein 6, putative
Plasmodium knowlesi PKNH_1142700   spindle assembly abnormal protein 6, putative
Plasmodium vivax PVX_113545   spindle assembly abnormal protein 6, putative
Plasmodium yoelii PY01398   hypothetical protein
Schistosoma japonicum Sjp_0218560   IPR008696,NAF1,domain-containing
Schistosoma japonicum Sjp_0309780   expressed protein
Schistosoma japonicum Sjp_0067770   Spindle assembly abnormal protein 6 homolog, putative
Schistosoma mansoni Smp_159110   dystonin
Schmidtea mediterranea mk4.013448.00   Spindle assembly abnormal protein 6 homolog
Schmidtea mediterranea mk4.011722.01  
Schmidtea mediterranea mk4.026484.00   Spindle assembly abnormal protein 6 homolog
Schmidtea mediterranea mk4.021442.00  
Schmidtea mediterranea mk4.006369.01  
Trypanosoma brucei gambiense Tbg972.9.6150   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.10550   Spindle assembly abnormal protein 6 homolog
Trypanosoma cruzi TcCLB.506147.100   Spindle assembly abnormal protein 6 homolog
Toxoplasma gondii TGME49_306430   hypothetical protein
Trichomonas vaginalis TVAG_188420   conserved hypothetical protein
Trichomonas vaginalis TVAG_587440   conserved hypothetical protein

Essentiality

LmjF.35.4280 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.1930 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.1930 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.1930 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.1930 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0106200 Plasmodium berghei Dispensable plasmo
TGME49_306430 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.35.4280 (Leishmania major), hypothetical protein, conserved
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