Detailed view for Smp_168160

Basic information

TDR Targets ID: 285685
Schistosoma mansoni, ubiquitin ligase E3a
Source Database / ID:  GeneDB

pI: 4.5872 | Length (AA): 1061 | MW (Da): 119523 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00632   HECT-domain (ubiquitin-transferase)
PF16558   Amino-terminal Zinc-binding domain of ubiquitin ligase E3A

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0016881   acid-amino acid ligase activity  
GO:0004842   ubiquitin-protein ligase activity  
GO:0006464   protein modification process  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 64 2kr1 (A) 4 60 46.00 0 1 0.618708 -0.89
83 994 1u6g (C) 16 1007 13.00 0 0.79 0.874666 0.58
84 385 4k6j (B) 745 1140 10.00 0 0.1 0.325737 -0.19
629 1056 5tj7 (A) 346 865 27.00 0 1 0.693393 -0.04
705 1055 1c4z (A) 497 846 57.00 0.0000000000069 1 1.05382 -0.87
949 1055 6fx4 (A) 0 112 44.00 0.0048 1 0.732848 -1.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127640)

Species Accession Gene Product
Brugia malayi Bm1_23895   ubiquitin-protein ligase E3A
Candida albicans CaO19.5892   potential ubiquitin-protein ligase similar to S. cerevisiae HUL4 (YJR036C) class E3 ubiquitin ligase
Candida albicans CaO19.13313   potential ubiquitin-protein ligase similar to S. cerevisiae HUL4 (YJR036C) class E3 ubiquitin ligase
Cryptosporidium hominis Chro.60288   e3 ubiquitin-protein ligase
Cryptosporidium parvum cgd6_2490   e3 ubiquitin-protein ligase, putative
Dictyostelium discoideum DDB_G0290179   ubiquitin-protein ligase E3A
Drosophila melanogaster Dmel_CG6190   Ubiquitin protein ligase E3A
Echinococcus granulosus EgrG_001045300   ubiquitin protein ligase E3A
Entamoeba histolytica EHI_187180   ubiquitin-protein ligase, putative
Entamoeba histolytica EHI_011530   ubiquitin-protein ligase, putative
Echinococcus multilocularis EmuJ_001045300   ubiquitin protein ligase E3A
Giardia lamblia GL50803_137754   Hypothetical protein
Homo sapiens ENSG00000114062   ubiquitin protein ligase E3A
Homo sapiens ENSG00000165338   HECT domain containing E3 ubiquitin protein ligase 2
Loa Loa (eye worm) LOAG_08458   hypothetical protein
Mus musculus ENSMUSG00000025326   ubiquitin protein ligase E3A
Mus musculus ENSMUSG00000041180   HECT domain containing 2
Neospora caninum NCLIV_001030   hypothetical protein
Neospora caninum NCLIV_005820   ubiquitin-transferase domain containing protein, putative
Saccharomyces cerevisiae YJR036C   putative E3 ubiquitin-protein ligase HUL4
Schistosoma japonicum Sjp_0121340   expressed protein
Schistosoma japonicum Sjp_0015420   ko:K10587 ubiquitin-protein ligase E3 A, putative
Schistosoma mansoni Smp_168160   ubiquitin ligase E3a
Toxoplasma gondii TGME49_294280   E3 ubiquitin-protein ligase, putative
Toxoplasma gondii TGME49_222430   HECT-domain (ubiquitin-transferase) domain-containing protein
Trichomonas vaginalis TVAG_093640   ubiquitin-protein ligase E3a, putative
Trichomonas vaginalis TVAG_496210   ubiquitin ligase E3a, putative
Trichomonas vaginalis TVAG_272190   ubiquitin-protein ligase E3a, putative
Trichomonas vaginalis TVAG_493990   hect E3 ubiquitin ligase, putative
Trichomonas vaginalis TVAG_078110   hect E3 ubiquitin ligase, putative
Trichomonas vaginalis TVAG_234440   ubiquitin-protein ligase E3a, putative
Trichomonas vaginalis TVAG_494030   ubiquitin-protein ligase, putative

Essentiality

Smp_168160 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_294280 Toxoplasma gondii Probably essential sidik
TGME49_222430 Toxoplasma gondii Probably essential sidik
TGME49_294280 Toxoplasma gondii Probably non-essential sidik
TGME49_222430 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Smp_168160 (Schistosoma mansoni), ubiquitin ligase E3a
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