Detailed view for LmjF.35.1420

Basic information

TDR Targets ID: 28581
Leishmania major, peroxisome targeting signal 1 receptor, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.206 | Length (AA): 631 | MW (Da): 70741 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13432   Tetratricopeptide repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

Peroxisome (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
213 630 1w3b (A) 15 393 14.00 0 1 0.74 0.28
315 426 1hxi (A) 334 445 70.00 0 1 1.15 -1.84
512 584 1na3 (A) 5 77 27.00 0.000000000023 1 0.65 -2.09
315 628 3cv0 (A) 334 651 66.00 0 1 1.28222 -1.06
459 540 5dse (A) 746 827 28.00 0.055 0.97 0.630552 -1.35
494 566 1elw (A) 21 93 23.00 0.22 0.91 0.527289 -1.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128408)

Species Accession Gene Product
Arabidopsis thaliana AT5G56290   peroxisome biogenesis protein 5
Brugia malayi Bm1_47095   TPR Domain containing protein
Candida albicans CaO19.5640   similar to S. cerevisiae PEX5 (YDR244W) receptor for peroxisomal targeting sequence 1
Candida albicans CaO19.13085   similar to S. cerevisiae PEX5 (YDR244W) receptor for peroxisomal targeting sequence 1
Caenorhabditis elegans CELE_C34C6.6   Protein PRX-5, isoform A
Dictyostelium discoideum DDB_G0286033   peroxin 5
Drosophila melanogaster Dmel_CG14815   Peroxin 5
Entamoeba histolytica EHI_179030   hypothetical protein, conserved
Homo sapiens ENSG00000139197   peroxisomal biogenesis factor 5
Leishmania braziliensis LbrM.34.1340   peroxisome targeting signal 1 receptor, putative
Leishmania donovani LdBPK_351430.1   peroxisome targeting signal 1 receptor, putative
Leishmania infantum LinJ.35.1430   peroxisome targeting signal 1 receptor, putative
Leishmania major LmjF.35.1420   peroxisome targeting signal 1 receptor, putative
Leishmania mexicana LmxM.34.1420   peroxisome targeting signal 1 receptor, putative
Loa Loa (eye worm) LOAG_07334   TPR Domain containing protein
Mus musculus ENSMUSG00000005069   peroxisomal biogenesis factor 5
Neospora caninum NCLIV_031880   TPR domain-containing protein, putative
Oryza sativa 4345945   Os08g0500100
Saccharomyces cerevisiae YDR244W   Pex5p
Schmidtea mediterranea mk4.012492.01   PEX5-related protein
Schmidtea mediterranea mk4.019214.01   PEX5-related protein
Trypanosoma brucei gambiense Tbg972.5.1520   peroxisome targeting signal 1 receptor, putative
Trypanosoma brucei Tb927.5.1100   peroxisome targeting signal 1 receptor
Trypanosoma congolense TcIL3000_5_910   peroxisome targeting signal 1 receptor
Trypanosoma cruzi TcCLB.511181.90   peroxisome targeting signal 1 receptor, putative
Trypanosoma cruzi TcCLB.508299.70   peroxisome targeting signal 1 receptor, putative
Toxoplasma gondii TGME49_231870   tetratricopeptide repeat-containing protein

Essentiality

LmjF.35.1420 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.1100 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.1100 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.1100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.1100 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C34C6.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_C34C6.6 Caenorhabditis elegans larval arrest wormbase
CELE_C34C6.6 Caenorhabditis elegans slow growth wormbase
TGME49_231870 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania donovani No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: targeted double KO of the PEX5 gene could not be generated. References: 10788481

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.35.1420 (Leishmania major), peroxisome targeting signal 1 receptor, putative
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