Detailed view for LmjF.05.0680

Basic information

TDR Targets ID: 28597
Leishmania major, sre-2/carboxylate carrier-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.2039 | Length (AA): 346 | MW (Da): 37885 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03820   Tricarboxylate carrier

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0015075   ion transmembrane transporter activity  
GO:0008324   cation transmembrane transporter activity  
GO:0055085   transmembrane transport  
GO:0006812   cation transport  
GO:0006811   ion transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
237 287 5b57 (A) 149 203 45.00 0.28 0.02 0.320499 2.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127740)

Species Accession Gene Product
Brugia malayi Bm1_35025   Sideroflexin 1
Brugia malayi Bm1_15865   hypothetical protein
Candida albicans CaO19.4811   similar to rat tricarboxylate carrier
Candida albicans CaO19.12274   similar to rat tricarboxylate carrier
Caenorhabditis elegans CELE_T04F8.1   Protein SFXN-1.5
Caenorhabditis elegans CELE_C41C4.10   Protein SFXN-5
Dictyostelium discoideum DDB_G0285029   tricarboxylate carrier family protein
Drosophila melanogaster Dmel_CG11739   CG11739 gene product from transcript CG11739-RA
Echinococcus granulosus EgrG_000833100   sideroflexin 1
Echinococcus granulosus EgrG_000759400   metaxin 2
Echinococcus multilocularis EmuJ_000759400   metaxin 2
Echinococcus multilocularis EmuJ_000833100   sideroflexin 1
Homo sapiens ENSG00000164466   sideroflexin 1
Homo sapiens ENSG00000144040   sideroflexin 5
Homo sapiens ENSG00000107819   sideroflexin 3
Leishmania braziliensis LbrM.05.0670   sre-2/carboxylate carrier-like protein
Leishmania braziliensis LbrM.01.0600   tricarboxylate carrier, putative
Leishmania donovani LdBPK_050680.1   sre-2/carboxylate carrier-like protein
Leishmania donovani LdBPK_010590.1   tricarboxylate carrier, putative
Leishmania infantum LinJ.05.0680   sre-2/carboxylate carrier-like protein
Leishmania infantum LinJ.01.0590   tricarboxylate carrier, putative
Leishmania major LmjF.01.0570   tricarboxylate carrier, putative
Leishmania major LmjF.05.0680   sre-2/carboxylate carrier-like protein
Leishmania mexicana LmxM.05.0680   sre-2/carboxylate carrier-like protein
Leishmania mexicana LmxM.01.0570   tricarboxylate carrier, putative
Loa Loa (eye worm) LOAG_04844   hypothetical protein
Loa Loa (eye worm) LOAG_14392   hypothetical protein
Loa Loa (eye worm) LOAG_16057   hypothetical protein
Mus musculus ENSMUSG00000021474   sideroflexin 1
Mus musculus ENSMUSG00000033720   sideroflexin 5
Mus musculus ENSMUSG00000025212   sideroflexin 3
Neospora caninum NCLIV_014380   tricarboxylate carrier, putative
Saccharomyces cerevisiae YOR271C   Fsf1p
Schistosoma japonicum Sjp_0202590   Sideroflexin-1, putative
Schistosoma mansoni Smp_136960   sideroflexin 123
Schmidtea mediterranea mk4.025378.00  
Schmidtea mediterranea mk4.001893.01   AT24389p
Trypanosoma brucei gambiense Tbg972.9.2260   tricarboxylate carrier, putative
Trypanosoma brucei Tb927.9.4310   tricarboxylate carrier, putative
Trypanosoma cruzi TcCLB.510153.19   tricarboxylate carrier, putative
Trypanosoma cruzi TcCLB.511583.40   tricarboxylate carrier, putative
Toxoplasma gondii TGME49_286060   tricarboxylate carrier protein

Essentiality

LmjF.05.0680 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.2910 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.160.2910 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.160.2910 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.2910 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YOR271C Saccharomyces cerevisiae inviable yeastgenome
TGME49_286060 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Lmaj00196AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj00196; Recombinant protein: full-length; Source: L major; sre-2/carboxylate carrier-like protein; old ID CAC44731.1 ;

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmjF.05.0680 (Leishmania major), sre-2/carboxylate carrier-like protein
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