Detailed view for LmjF.10.0560

Basic information

TDR Targets ID: 28925
Leishmania major, zinc binding dehydrogenase-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2441 | Length (AA): 371 | MW (Da): 39654 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00107   Zinc-binding dehydrogenase
PF08240   Alcohol dehydrogenase GroES-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0008270   zinc ion binding  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 370 2c0c (A) 11 362 19.00 0 1 1.08 -0.49
14 366 1yb5 (A) 8 327 26.00 0 1 1.24 -0.73
162 337 1pqw (A) 15 192 24.00 0.0000000000031 1 0.93 -1.53
2 368 4dl9 (A) 1 377 20.00 0 1 0.944518 0.85
14 365 5a3v (A) 6 327 26.00 0 1 1.18809 0.21
30 236 5a3v (A) 18 206 42.00 0 1 0.817851 0.68
162 337 4oki (A) 671 848 23.00 0.00000000046 1 0.855694 -0.81

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129998)

Species Accession Gene Product
Candida albicans CaO19.5978   one of six potential zinc-binding dehydrogenase genes similar to S. cerevisiae YNL134C
Candida albicans CaO19.13399   one of six potential zinc-binding dehydrogenase genes similar to S. cerevisiae YNL134C
Leishmania braziliensis LbrM.10.0690   zinc binding dehydrogenase-like protein
Leishmania braziliensis LbrM.10.0700   zinc-binding dehydrogenase-like protein
Leishmania donovani LdBPK_100610.1   zinc binding dehydrogenase-like protein
Leishmania donovani LdBPK_100620.1   zinc-binding dehydrogenase-like protein
Leishmania infantum LinJ.10.0620   zinc-binding dehydrogenase-like protein
Leishmania infantum LinJ.10.0610   zinc binding dehydrogenase-like protein
Leishmania major LmjF.10.0570   zinc-binding dehydrogenase-like protein
Leishmania major LmjF.10.0560   zinc binding dehydrogenase-like protein
Leishmania mexicana LmxM.10.0570   zinc-binding dehydrogenase-like protein
Leishmania mexicana LmxM.10.0560   zinc binding dehydrogenase-like protein
Oryza sativa 4347496   Os09g0503100
Trypanosoma brucei gambiense Tbg972.8.3710   oxidoreductase, putative
Trypanosoma brucei Tb927.8.3970   oxidoreductase, putative
Trypanosoma cruzi TcCLB.507623.50   oxidoreductase, putative
Trypanosoma cruzi TcCLB.505943.20   oxidoreductase, putative

Essentiality

LmjF.10.0560 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3970 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3970 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.3970 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3970 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Lmaj010130AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj010130; Recombinant protein: full-length; Source: L major; zinc binding dehydrogenase-like protein ;

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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