Detailed view for LmjF.36.2540

Basic information

TDR Targets ID: 29041
Leishmania major, C-4 sterol methyl oxidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.6352 | Length (AA): 402 | MW (Da): 45925 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04116   Fatty acid hydroxylase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0005783   endoplasmic reticulum  
GO:0016491   oxidoreductase activity  
GO:0005506   iron ion binding  
GO:0055114   oxidation reduction  
GO:0008610   lipid biosynthetic process  
GO:0006633   fatty acid biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
139 247 4nqq (A) 61 174 29.00 0.35 0.18 0.159344 2.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129113)

Species Accession Gene Product
Arabidopsis thaliana AT1G07420   sterol 4-alpha-methyl-oxidase
Arabidopsis thaliana AT2G29390   sterol 4-alpha-methyl-oxidase 2-2
Candida albicans CaO19.12101   second version of C-4 sterol methyl oxidase
Candida albicans CaO19.11216   C-4 methyl sterol oxidase
Candida albicans CaO19.3732   C-4 methyl sterol oxidase
Candida albicans CaO19.4631   second version of C-4 sterol methyl oxidase
Dictyostelium discoideum DDB_G0270946   C-4 methyl sterol oxidase
Dictyostelium discoideum DDB_G0269788   C-4 methyl sterol oxidase
Drosophila melanogaster Dmel_CG11162   CG11162 gene product from transcript CG11162-RA
Homo sapiens ENSG00000052802   methylsterol monooxygenase 1
Leishmania braziliensis LbrM.35.2750   C-4 sterol methyl oxidase, putative;with=GeneDB:LmjF36.2540
Leishmania donovani LdBPK_362670.1   C-4 sterol methyl oxidase, putative
Leishmania infantum LinJ.36.2670   C-4 sterol methyl oxidase, putative;with=GeneDB:LmjF36.2540
Leishmania major LmjF.36.2540   C-4 sterol methyl oxidase, putative
Leishmania mexicana LmxM.36.2540   hypothetical protein, unknown function
Mus musculus ENSMUSG00000031604   methylsterol monoxygenase 1
Oryza sativa 4351226   Os11g0707600
Oryza sativa 4342171   Os07g0101500
Saccharomyces cerevisiae YGR060W   methylsterol monooxygenase

Essentiality

LmjF.36.2540 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
YGR060W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.36.2540 (Leishmania major), C-4 sterol methyl oxidase, putative
Title for this comment
Comment